Charmi Chande

New Jersey Institute of Technology | NJIT · Department of Chemical Biological and Pharmaceutical Engineering

Point of care (POC) diagnostic devices provide a method for rapid accurate identification of disease through analysis of biologically relevant substances. This review focuses on the utility of POC testing for early detection of periodontitis, a critical factor in treating the disease. Accessing the oral cavity for biological sampling is less invasi...

The most well-known criterion for POC devices is ASSURED, and affordability, i.e., using low-cost instrumentation, is the most challenging one. This manuscript provides a pathway for transitioning ESSENCE, an impedance-based biosensor platform, from using an expensive benchtop analyzer—KeySight 4294A (~$50k)—to using a significantly portable and ch...

This review paper presents the recent developments in spectroelectrochemical (SEC) technologies. The coupling of spectroscopy and electrochemistry enables SEC to do a detailed and comprehensive study of the electron transfer kinetics and vibrational spectroscopic fingerprint of analytes during electrochemical reactions. Though SEC is a promising te...

With the rapid growth of emerging point-of-use (POU)/point-of-care (POC) detection technologies, miniaturized sensors for the real-time detection of gases and airborne pathogens have become essential to fight pollution, emerging contaminants, and pandemics. However, the low-cost development of miniaturized gas sensors without compromising selectivi...

The ESSENCE platform is an electrochemical sensor system that utilizes non-planar porous microelectrode microfluidic chips with a fully programmable fluidic/sample system to be a “portable, automatic, rapid, sensitive, and selective” for different emerging contaminants and liquid biopsy. The system is designed with non-planar porous microelectrode...

An NP-μFEC is a reusable, novel microfluidic electrochemical cell with multiple non-planar interdigitated microelectrode arrays, minimal sample volume, and enhanced electric field penetration for highly sensitive electrochemical analysis. (i) The NP-μFEC features spatial 3-electrode architecture, and a small sample volume (∼4 μL). (ii) Here, [Fe(CN...

Microtoxicology is concerned with the toxic effects of small amounts of substances. In this progress report, we discuss the application of small amounts of noxious substances for toxicological investigation in...

We present a new methodological approach for the assessment of the susceptibility of Rhodococcus erythropolis strains from specific sampling sites in response to increasing heavy metal concentration (Cu2+, Ni2+, and Co2+) using the droplet-based microfluid technique. All isolates belong to the species R. erythropolis identified by Sanger sequencing...

The rapid, sensitive, and selective detection of target analytes using electrochemical sensors is challenging. ESSENCE, a new Electrochemical Sensor that uses a Shear-Enhanced, flow-through Nanoporous Capacitive Electrode, overcomes current electrochemical sensors' response limitations, selectivity, and sensitivity limitations. ESSENCE is a microfl...

Microfluidic devices are constructed from polydimethylsiloxane (PDMS) due to their biocompatibility, fabrication ease, well-established protocols, and simplicity. PDMS-based microfluidic devices are constructed by (i) applying liquid PDMS to a negative mold (usually a silicon or 3D-printed mold) and (ii) curing the PDMS with heat exposure over a se...

The current paper describes cationic polymerization reaction of a negative photo resist formulated with mixture of DGEBA (Diglycidyl Ether of Bisphenol-A) and Triarylsulfonium hexafluorophosphate salts. FTIR and UV visible analysis was carried out to understand the crosslinking reaction. The formulated photo resist material was used to fabricate hi...

We propose a low cost and wieldy photolithography method which circumvent the need of expensive fabrication tools like mask aligner and EBL (Electron beam lithography) systems, making it possible to use this protocol in resource constraints laboratories. The proposed protocol involves use of a spinner and an Osram (Ultra Vitalux) lamp along with an...

Researchers have been developing numerous advance microfabrication technologies for studying eukaryotic cells on chip. Developing similar protocol for prokaryotic organisms is difficult due to various reasons like small feature size, similar physical and chemical properties, high environmental diversity, etc. By using classical microbiology experim...