Catherine Charreyre

BIAH · R&D

The Virology Department, VSD, DARDNI, in collaboration with colleagues in Europe and N America has had an active research programme on porcine circovirus-related diseases, including PMWS, for the last 5 years. This presentation will highlight some of our on-going research in this area.

New strategies for innovative vaccination and their applications in veterinary medecine. For the last 100 years, vaccination has had a tremendous positive impact on the health of humans and animals. Looking at the recent advances in our understanding of immunological mechanisms, and taking into account the possibility to combine several different s...

Hendra virus (HeV) is an emerging zoonotic pathogen, which causes severe respiratory illness and encephalitis in humans and horses. Since its first appearance in 1994, spillovers of HeV from its natural reservoir fruit bats occur on almost an annual basis. The high mortality rate in both humans and horses and the wide-ranging reservoir distribution...

Antibodies play a pivotal role against viral infection, and maintenance of protection is dependent on plasma and memory B cells. Understanding antigen-specific B cell responses in cattle is essential to inform future vaccine design. We have previously defined T cell-dependent and -independent B cell responses in cattle, as a prelude to investigatin...

We investigated porcine circovirus type 2 (PCV2) virological profiles in herds affected (PCVAD-AH, n = 5) or non-affected (PCVAD-NAH, n = 4) by PCV2-associated diseases (PCVAD), before and after 1 y of PCV2 gilt and sow vaccination. Fresh feces from the floor (5 pens/age/farm) and 5 blood samples (1/pen) were collected at 3, 9, 15, 21 wk. Individua...

Snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and Erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. Following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples t...

Porcine circovirus type 2 (PCV2) is a ubiquitous virus that mainly affects nursery and fattening pigs causing systemic disease (PCV2-SD) or subclinical infection. A characteristic sign in both presentations is reduction of average daily weight gain (ADWG). The present study aimed to assess the relationship between PCV2 load in serum and ADWG from 3...

Background Porcine circovirus type 2 (PCV2) is the causal agent of postweaning multisystemic wasting syndrome (PMWS), which has severely impacted the swine industry worldwide. PCV2 triggers a weak and atypical innate immune response, but the key genes and mechanisms by which the virus interferes with host innate immunity have not yet been elucidate...

In this study we detail the detection and genetic analysis of a novel porcine boca-like virus (PBo-likeV) in archival sera and tissue samples from pigs from farms in Great Britain. We also investigate the distribution of porcine circovirus type 2 (PCV2) genotypes and Torque teno sus virus (TTSuV) genogroups 1 and 2 in combination with this novel PB...

Objective: To characterize the kinetics of interleukin (IL)-4, IL-5, and IL-13 secretion in peripheral blood and lymph node mononuclear cells isolated from porcine circovirus type 2 (PCV2)-vaccinated pigs after cells were challenged with PCV2 open reading frame 2 antigen. Animals: 10 pigs. Procedures: 5 pigs were vaccinated with a PCV2 vaccine...

Understanding the mechanisms that maintain protective antibody levels after immunisation is important for vaccine design. In this study, we have determined the kinetics of plasma and memory B cells detectable in the blood of cattle immunised with model T-dependent or T-independent antigens. Immunisation with the T-D antigen resulted in an expansion...

Haemophilus parasuis (H. parasuis) is the etiological agent of Glässer's disease in pigs. Currently, the molecular basis of this infection is largely unknown. The innate immune response is the first line of defense against the infectious disease. Systematical analysis on host innate immune response to the infection is important for understanding th...

SIR, — To date, at least three different phylogenetic groups of porcine circo-virus type 2 (pcv-2) have been recognised ([Gagnon and others 2007][1], [Dupont and others 2008][2], [Grau-Roma and others 2008][3], [Timmusk and others 2008][4]). However, the mandate of the International Committee on

Cell-mediated immunity and humoral responses in pigs vaccinated with inactivated vaccine + adjuvant (PROGRESSIS)

Providing protective immunity to neonatal animals in early life is associated with numerous challenges regarding vaccine safety and efficacy. A much simpler approach is maternal vaccination, either before or during pregnancy, to provide the neonate with passively transferred immunity. In humans, the medical, societal and legal risks of immunizing p...

Although field studies have found porcine reproductive and respiratory syndrome (PRRSV) inactivated vaccines to be beneficial in reducing losses linked to PRRSV infection, immune mechanisms induced by these vaccines need better understanding. In the study reported here, we examined the interferon-gamma(+) (IFNgamma(+)) PRRS-specific T cell response...

Porcine circovirus type 2 (PCV2) is now recognised as the causal agent of porcine multisystemic wasting syndrome (PMWS), an economically important wasting disease of young pigs [J. Vet. Diagn. Invest. 12 (2000) 3]. Gross lesions of PMWS include generalised lymphadenopathy, hepatitis, nephritis and pneumonia and typical histological lesions include...

The objective of this study was to test the compliance of RUVAX® with the specifications of the European Pharmacopoeia (Ph. Eur.) monograph No. 0064 (1, 2). Vaccines: RUVAX ® is an aluminium-hydroxide adjuvanted erysipelas vaccine for pigs. Two different vaccines were prepared with graded Erysipelas antigen content (“1:6 Ery.” and “1:10 Ery.”). The...

The objective of this study was to test the compliance of PARVORUVAX® with the specifications of the European Pharmacopoeia (Ph. Eur.) monograph No. 0064 (1, 2). One hundred percent of the control pigs showed typical local lesions exceeding 10cm of diameter on both infection sites. Twenty percent of the control pigs showed dermatitis generalization...

The objective of the present study was to evaluate the ORF5 gene genetic variability of 11 European PRRSV field strains in comparison with the European prototype Lelystad virus (LV) and the U.S. ATCC reference strain VR2332. For this purpose, we modified the method of WESLEY et al. [19, 20] based on reverse transcriptase-polymerase chain reaction (...

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based t...

Fifteen specific-pathogen-free cats were experimentally infected with FeLV; 8 cats recovered after transient or nondetectable viremia, and 7 cats became persistently viremic. Four additional cats served as noninfected controls. Antibodies to whole FeLV (ELISA and immunoblot [western] analysis), antibodies to fixed FeLV-infected cells, and virus-neu...

Introduction Esophagogastric ulcer is a widespread disease affecting pigs of different ages, especially to finishers. The ethiology of the disease is multifactorial, influencing nutritional, genetic, pathologic and management factors on its development and severity. The pathogeny has not been completely understood yet (1). The aim of this study was...