Carla Matte

Universidade Federal do Rio Grande do Sul | UFRGS · Departamento de Tecnologia dos Alimentos

Endophytic fungi (EF) have a notable capacity to produce active molecules of industrial importance, such as hydrolytic enzymes. In this study we investigated the production of lipase by EFs isolated from the Amazonian species Aniba canelilla (Lauraceae), characterized the enzymatic extract obtained from the most promising fungus, and applied the li...

The dairy bacteria Propionibacterium sp. and Acidipropionibacterium sp. are versatile and potentially probiotic microorganisms showing outstanding functionalities for the food industry, such as the production of propionic acid and vitamin B12 biosynthesis. They are the only food grade microorganisms able to produce vitamin B12. However, the ferment...

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch controlled as DO-stat strategies were employed for the production of the recombinant enzyme. In 30 h-batch cultivatio...

We investigated the changes in the physical structure of cellulose recovered from soybean and rice hulls treated with the ionic liquids 1-butyl-3-methylimidazolium chloride ([bmim][Cl]) and 1-butyl-3-methylimidazolium acetate ([bmim][Ac]). The characterization was carried out by a combination of thermogravimetric analysis (TGA), Fourier transform i...

The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated...

Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML...

Vitamin B12 deficiency still persists, mainly caused by low intake of animal food products affecting vegetarians, vegans, and populations of underdeveloped countries. In this study, we investigate the biosynthesis of vitamin B12 by potential probiotic bacterium using an agroindustry residue, the liquid acid protein residue of soybean (LAPRS), as a...

The transglutaminases form a large family of intracellular and extracellular enzymes that catalyze cross-links between protein molecules. Transglutaminases crosslinking properties are widely applied to various industrial processes, to improve the firmness, viscosity, elasticity, and water-holding capacity of products in the food and pharmaceutical...

Because of their protein cross-linking properties, transglutaminases are widely used in several industrial processes, including the food and pharmaceutical industries. Transglutaminases obtained from animal tissues and organs, the first sources of this enzyme, are being replaced by microbial sources, which are cheaper and easier to produce and puri...

We applied ultrasonic technology for the transesterification of soybean oil catalyzed by a mixture of lipases (combi-lipase: 75 % Novozym 435; 10 % Lipozyme TL-IM; and 15 % Lipozyme RM-IM), verifying the effects of ultrasonic amplitude, pulse conditions, the ethanol:oil molar ratio, enzyme concentration, and the influence of solvent (tert-butanol o...

This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was performed in a mixture design of three...

This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The influence of the addition of glass beads to the reactor bed, evaluation of the us...

We carried out the physico-chemical characterization of Immobead 150, a hydrophobic support for the immobilization of lipases. Thermomyces lanuginosus lipase (TLL) was immobilized on Immobead 150 by multipoint covalent attachment (ImmTLL) and the morphological, textural, structural, thermal, and physico-chemical properties of the support, before an...

This study reports the immobilization of a β-CGTase on glutaraldehyde pre-activated silica and its use to production of cyclodextrins in batch and continuous reactions. We were able to modulate the cyclodextrin production (α-, β- and γ-CD) by immobilization and changing the reaction conditions. In batch reactions, the immobilized enzyme reached to...

In this study, two different approaches were assessed in order to direct the immobilization of a cyclodextrin glycosyltransferase on functionalized silica support, one by amino groups using glutaraldehyde activation (Si-NH-G-CGTase) and other by disulfide bond through the Cys on the enzyme surface (Si-SH-CGTase). The efficiency of the immobilizatio...

Thermomyces lanuginosus lipase (TLL) was covalently immobilized on Immobead 150 (ImmTLL) via epoxy groups of the support and the lysine of the enzyme to be used in the synthesis of butyl butyrate. The optimization of the esterification reaction parameters was carried out by central composite design and response surface methodology, having product y...

Thin films containing silver nanoparticles homogeneously dispersed, with narrow size distribution below 10 nm, were synthesized on flat glass surface, by using an ionic silsesquioxane as stabilizer and crosslinking agent. The films can be prepared without previous functionalization of substrate surfaces and without addition of other components. The...

Although reactions of transesterification are generally catalyzed by one specific lipase preparation, the concept of “combi-lipase” could be better explored for the production of biodiesel, since oils are heterogeneous substrates. In this research, we tested this concept by evaluating the enzymatic transesterification of olive and palm oils, two di...

Thermomyces lanuginosus lipase (TLL) was immobilized on native and modified Immobead 150, with epoxy groups removed by hydrolysis and oxidized to add aldehyde on its surface. Immobilizations on both supports were performed by adsorption, adsorption and cross-linking, covalent attachment, multipoint covalent attachment, and, for the modified support...

In this work two immobilized preparations of lipase (EC 3.1.1.3) B from Candida antarctica (CALB) were compared for the synthesis of fatty acid methyl esters (FAME) using soybean oil. Commercial Novozym 435 (CALB-435) and CALB immobilized on styrene-divinylbenzene beads (CALB-MCI) were tested for the transesterification reactions. Central composite...

In this work, the enzymatic properties of immobilized cyclodextrin glycosyltransferase (CGTase) of Thermoanaerobacter sp. were investigated and compared with the soluble form of the enzyme. CGTase was immobilized on mesoporous silica microspheres synthesized using polyethylene glycol 400 as swelling agent, silanized with 3-aminopropyltrimethoxysila...