Breno Pupin

Universidade do Vale do Paraíba | univap · Ciências Biológicas

The enormous soil carbon pool stored by mangroves depends upon microbial respiratory activities and enzymes associated with carbon breakdown in soils. Our hypothesis is that increased microbial activity leads to elevated carbon breakdown. To prove this, we measured microbial respiratory and enzyme activity for nitrogen, phosphorus, and carbon cycle...

We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30...

We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30...

Alternative therapies against pathogens are under intense investigation because of their increasing resistance to antibiotics. Photodynamic therapy (PDT) is one such alternative that has shown promising results. However, for the widespread use of PDT, it is essential to decipher underlying mechanisms, so as to improve PDT’s therapeutic applications...

White light during mycelial growth influences high conidial stress tolerance of the insect-pathogenic fungus Metarhizium robertsii, but little is known if low- or high-white light irradiances induce different stress tolerances. The fungus was grown either in the dark using two culture media: on minimal medium (Czapek medium without sucrose = MM) or...

This study evaluated the use of hydrogel on the development of Rapanea ferruginea under water restriction through Vibrational Fourier Transform Infrared Spectroscopy (FTIR-UATR). Seedlings of approximately 30 cm height were transferred in pots with 3 L of soil. The group of seedlings was separated into 5 different triplicate treatments according to...

Light is an important signal for fungi in the environment and induces many genes with roles in stress and virulence responses. Conidia of the entomopathogenic fungi Aschersonia aleyrodis, Beauveria bassiana, Cordyceps fumosorosea, Lecanicillium aphanocladii, Metarhizium anisopliae, Metarhizium brunneum, Metarhizium robertsii, Simplicillium lanosoni...

Differential sensitivities to the cell wall stress caused by Congo red (CR) have been observed in many fungal species. In this study, the tolerances and sensitivities to CR was studied with an assorted collection of fungal species from three phylogenetic classes: Sordariomycetes, Dothideomycetes, and Eurotiomycetes, three orders, and eight families...

Background Approximately 70% of cervical carcinoma cases show the presence of high-risk Human Papilloma Virus (HPV), especially HPV-16 and HPV-18, and can be used to stratify high risk patients from low risk and healthy. Currently, molecular biology techniques such as polymerase chain reaction (PCR) are used to identify the presence of virus in pat...

This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance-Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) o...

The effects of the visible light wavelengths on germination, mycelial radial growth, and conidial production of the plant pathogens Colletotrichum acutatum and Fusarium fujikuroi were studied. Both fungi were grown on potato dextrose agar medium (PDA) in the dark (control) or on PDA under continuous white, blue, green or red light. In addition, the...

Desde a antiguidade as ervas aromáticas são utilizadas, não somente para melhorar o sabor e odor em alimentos, mas também para estender o tempo de prateleira com suas propriedades antissépticas. O aumento da utilização das ervas aromáticas na culinária tem sido aprimorado praticamente no mundo todo e principalmente na gastronomia. Entre as ervas ma...

Saliva has garnered a lot of interest as a non-invasive, easy to collect, and biochemical rich sample for attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) based disease diagnosis. Although a large number of studies have explored its potential, the preparation methods used differ greatly. For large scale clinical studie...

Thyroid cancer has become in recent years the most common endocrine malignancy. Among its different types, papillary thyroid carcinoma (PTC) has the highest incidence. PTC is slow growing, but shows a high rate of lymph node metastasis. Tissue biochemical characterization and identification of molecular markers can facilitate stratification of pati...

O câncer de tireoide é a neoplasia mais comum do sistema endócrino, apresentando incidência crescente nos últimos anos. O diagnóstico é realizado principalmente pela associação de dois métodos: a ultrassonografia e a punção aspirativa por agulha fina (PAAF); porém ambos apresentam uma elevada taxa de resultados indeterminados. Deste modo, é necessá...

Osmotic stress induced by high solute concentration can prevent fungal metabolism and growth due to alterations in properties of the cytosol, changes in turgor, and the energy required to synthesize and retain compatible solutes. We used germination to quantify tolerance/sensitivity to the osmolyte KCl (0.1–4.5 M, in 0.1 M increments) for 71 strain...

Fungi sense light and utilize it as a source of environmental information to prepare against many stressful conditions in nature. In this study, Metarhizium robertsii was grown on: 1) potato dextrose agar medium (PDA) in the dark (control); 2) under nutritive stress in the dark; and 3) PDA under continuous (A) white light; (B) blue light lower irra...

Survival of entomopathogenic fungi under solar ultraviolet (UV) radiation is paramount to the success of biological control of insect pests and disease vectors. The mutagenic compound 4-nitroquinoline 1-oxide (4-NQO) is often used to mimic the biological effects of UV radiation on organisms. Therefore, we asked whether tolerance to 4-NQO could pred...

The low survival of insect-pathogenic fungi when used for insect control in agriculture is mainly due to the deleterious effects of ultraviolet radiation and heat from solar irradiation. In this study, conidia of 15 species of entomopathogenic fungi were exposed to simulated full-spectrum solar radiation emitted by a Xenon Test Chamber Q-SUN XE-3-H...

Microorganisms are essential to the functionality of the soil, particularly in organic matter decomposition and nutrient cycling, which regulate plant productivity and shape the soil structure. However, biotic and abiotic stresses may greatly disrupt soil fungal communities and, thereby, disturb the ecosystem. This study quantified seasonal toleran...

The availability of phosphorus (P) for the growth of plants often depends on the solubilisation of inorganic phosphates (P fractions) in the soil. The aim of this study was to investigate soil P fractions in terms of availability and soil characteristics of mangrove soil compared with the Atlantic forest and restinga soils that occur at Cardoso Isl...

Mangroves provide a distinctive ecological environment that differentiates them from other ecosystems. This study deal to evaluate the frequency of microbial groups and the metabolic activities of bacteria and fungi isolated from mangrove, restinga and Atlantic forest soils. Soil samples were collected during the summer and winter at depths of 0-2,...

Sugarcane culture is replanted after five–eight successive harvests and intensely fertilised and mechanised. The influence on bacteria (total, nitrifying, denitrifying), fungi, microbial biomass-C, and dynamic processes (respiratory activity, N mineralisation, potential nitrification, P-solubilising activity) and enzymatic activities (dehydrogenase...

A compactação é um dos fatores mais agravantes para a qualidade do solo, porém o seu efeito na comunidade e atividade enzimática microbiana não tem sido suficientemente estudado. Seis níveis de compactação foram obtidos pela passagem de tratores com diferentes pesos em um Latossolo Vermelho, e a densidade final foi medida. Amostras de solo foram co...