Andras Jozsef Laki

Pázmány Péter Catholic University · Faculty of Information Technology

Organ-on-a-chip technologies show exponential growth driven by the need to reduce the number of experimental animals and develop physiologically relevant human models for testing drugs. In vitro, microfluidic devices should be carefully designed and fabricated to provide reliable tools for modeling physiological or pathological conditions and asses...

The efficacy of transdermal absorption of drugs and the irritation or corrosion potential of topically applied formulations are important areas of investigation in pharmaceutical, military and cosmetic research. The aim of the present experiments is to test the role of P-glycoprotein in dermal drug delivery in various ex vivo and in vitro platforms...

To develop proper drug formulations and to optimize the delivery of their active ingredients through the dermal barrier, the Franz diffusion cell system is the most widely used in vitro/ex vivo technique. However, different providers and manufacturers make various types of this equipment (horizontal, vertical, static, flow-through, smaller and larg...

Various hydrophilic polymers have been reported to induce the formation of an exclusion zone (EZ) at their surface, which is devoid of particles and may extend to several hundred microns. It has previously been suggested that even cells may be excluded from the vicinity of the gel, thus raising the possibility of developing microscale cell-separati...

Label-free isolation and detection of extracellular vesicles are essential in many diagnostic and therapeutic approaches. Recently, there has been an interest in methods that avoid the use of biochemical labels, intrinsic biomarkers, or electrical polarizability to identify microvesicles (also referred to as microparticles) from serological samples...

We demonstrate a novel microfluidic device to detect living parasites from native blood using monolithic polydimethylsiloxane (PDMS) structures. Several blood-borne and tick-borne parasites such as dirofilariosis can be investigated by this microcapillary system. Inside this microfluidic device, a special flow-through separator structure has been i...

We demonstrate a novel integrated microfluidic device to separate circulating extracellular vesicles from blood stream using the deterministic lateral displacement principle. The device continuously fractionates extracellular vesicles and cells according to size and membrane flexibility by displacing them perpendicularly to the fluid flow direction...

We demonstrate a novel integrated microfluidic device to separate circulating extracellular vesicles from blood stream using the deterministic lateral displacement principle. The device continuously fractionates extracellular vesicles and cells according to size and membrane flexibility by displacing them perpendicularly to the fluid flow direction...

The exclusion zone (EZ) is a boundary region devoid of macromolecules and microscopic particles formed spontaneously in the vicinity of hydrophilic surfaces. The exact mechanisms behind this remarkable phenomenon are still not fully understood and are debated. We measured the short- and long-time-scale kinetics of EZ formation around a Nafion gel e...

We present the use of a simple microfluidic technique to detect living parasites from veterinarian blood using monolithic polydimethylsiloxane (PDMS) structures. Several blood borne parasites such as dirofilariosis or Lyme disease can be observed by this microcapillary system. Inside this microfluidic device a special flow-through separator structu...

A microfluidic analyzer system and a cell detection algorithm were developed to analyze biomedical fluids. The obtained microfluidic device is based on the integration of different fluidic systems: the SensoNor glass/silicon/glass multilayer microchip and ThinXXS plastic slide. A hydrodynamic focuser was designed to sort and analyze cells/particles...

In our project a standard microfluidic analyzer background system and its construction steps were developed to analyze biologic fluids. The obtained micro-Total-Analysis-System (μTAS) is based on the integration of different microflu-idic systems. Each part follows well-defined rules to make the integration of the large-scale production microchip t...