Ana Dobreva

Institute for Roses and Aromatic Plants Kazanlak Bulgaria

Essential oils (EOs) extracted from aromatic and medicinal plants have the potential to inhibit the growth of various pathogens and, thus, be useful in the control of dangerous diseases. The application of environmentally friendly approaches to protect agricultural and forestry ecosystems from invasive and hazardous species has become more signific...

Rosa centifolia L. and Rosa gallica L. (Rosaceae) are grown as raw materials for valuable essential oils and hydrosols. There are scarce data about the biological activities and the genoprotective potential of the hydrosols of these roses. The aim of the study was to provide information on their cytotoxic/genotoxic activity and anti-cytotoxic/anti-...

Increasing prices and market demand for organic products are stimulants of organic farming. However, this sector is a challenge for producers and further improvements are still necessary. The present study case was conducted to compare the effects of organic (OF) and conventional (CF) farming on lavender (Lavandula angustifolia Mill.) oil yield, pl...

Lactiplantibacillus plantarum is a versatile specie, well known as a producer of lactic acid (LA) and other metabolites with biotechnological significance. The present work characterizes growth and lactic acid production of the candidate-probiotic strain L. plantarum AC131, from Bulgarian white brined cheeses. Different nutritional media with ingre...

Bulgaria is known for its essential oils and medicinal plants, which occupy increasing parts in the agricultural areas. In recent years, the boom in lavender production has made our country the largest producer of lavender oil in the world. This intensified the scientific work with it and led to the creation of new lavender varieties and lines that...

Bulgarian Rosa damascena Mill. is has been known since ancient times for its high-quality oil, hydrosol, and other aromatic products. Rose hydrosol has various biological activities, but no research on its anticytotoxic/antigenotoxic effects exists. This study aimed to assess its defense potential against the genotoxin N-methyl-N′-nitro-N-nitrosogu...

Bulgaria is famous for its oil-bearing rose. R. damascena Mill. and R. alba L. are mainly cultivated in the country, but a recent survey of industrial plantations in 2020 revealed that R. centifolia L. and hybrids of R. damascena Mill. X R. gallica L. are also common in the rose valley. Although their essential oil cannot be compared in quality wit...

Bulgaria is famous for its oil-bearing rose. R. damascena Mill. and R. alba L. are mainly cultivated in the country, but a recent survey of industrial plantations in 2020 revealed that R. centifolia L. and hybrids of R. damascena Mill. x R. gallica L. are also common in the rose valley. Although their essential oil cannot be compared in quality wit...

The chemical composition and aroma profile of industrial essential oils (EOs) from species of rose grown in China, including the native Kushui rose (R. sertata × R. rugosа) and R. rugosa Thunb. cv. Plena, and the recently introduced Damask rose (R. damascena Mill.), were studied in comparison by means of GC/MS and GC-FID. More than 150 individual c...

A considerable amount of wastewater is produced during water-steam distillation of rose oil. This represents an environmental problem that should not be neglected. However, since this wastewater contains many bioactive substances, other targeted uses should be considered. Indeed, it should be checked whether this wastewater is harmless. In this sen...

The Rosa alba L. and Rosa damascena Mill. growing in Bulgaria are known for their extremely fine essential oil and valuable hydrosols. Irrespectively of its wide use in human life, little research exists on the cytotoxic and genotoxic activity of the hydrosols. This set our goal to conduct cytogenetic analyses to study these effects. A complex of c...

The aerial parts of Artemisia annua L. from Bulgaria were extracted with 1,1,1,2-tetrafluoroethane. Static two-stage extraction at a pressure of 8 and 12 bar was used at a relatively low temperature. The yield was 2%. The chemical profile of the product was reported for the first time. GC-MS analysis showed that the extract contained the essential...

The aim of this study was to establish whether the type of the agricultural system has any influence on the essential oil production and antioxidant activity of industrial cultivated Rosa damascena Mill. in the Rose valley, Bulgaria. Six private farms from Kazanlak (Rose) Valley, Southern Bulgaria were included in the study conducted in the period...

The wastewater after rose oil distillation is usually discharged into the drainage systems and it represents a serious environmental problem. While being rich in polyphenols, which have beneficial biological activity and application in the pharmaceutical industry, limited research has been carried out about the biological activity of the specific w...

The steam distillation of valuable rose essential oil from R. damascena Mill. and R. alba L. generates large volumes of wastewaters. Although such wastewaters are bio-pollutants, they contain valuable bioactive compounds. In this study we investigated the cytotoxic/genotoxic and anti-cytotoxic/anti-genotoxic potential of these products. We used cyt...

During the processing of rose flowers rose oil, rose concrete, rose absolute, and rose water are obtained. Waste rose flowers after the distillation of the essential oil still have a limited usage. The objective of the present study is to determine the chemical composition, in vitro gas production, digestibility and relative feed value of waste flo...

The process of essential oil water-steam distillation leaves a water fraction as a rest material of the technological process. This residual fraction represents a serious environmental pollutant. The aim of the present study was to investigate the clastogenic and cytotoxic effects of R. alba L. distillation wastewater in laboratory animal's test mo...

During the period 2014 – 2015 the herbicidal effect and the selectivity of isoxaflutole (Merlin 750 WG), oxadiargyl (Raft 400 SC), imazamox (Pulsar 40) and flumioxazine (Pledge 50 VP) were studied on lavender fields, Hemus and Jubilejna varieties. The present work focuses on the influence of low doses of the applied preparations on the yield and co...

The genus Lavandula includes a variety of species under the common name lavender. Bulgaria has a tradition in the production of high-quality lavender oil. This is the result of many years of selection work, which includes the study of introduced samples and their adaptogenic abilities in the country habitat. Three samples of Lavandula angustifolia...

The essential oils (EOs) and other extracts derived from aromatic and medicinal plants are presumed efficient natural agents for controlling the growth of pathogenic microorganisms. These plant products are considered as potential alternative of the chemical preparations broadly used in the plant protection practice. The inhibitory activity of plan...

The wastewater from the distillation of rose oils is discharged directly into the soil because it has a limited potential for future applications. The aim of the present study was to determine in vitro the chromatographic profile, redox-modulating capacity, and antineoplastic activity of wastewater obtained by distillation of essential oils from th...

The growth response of three phytopathogens to inhibitory activity of essential oils (EOs), derived from three monarda (Monarda didyma L.) genotypes, was evaluated. The antimicrobial effect was assayed by agar diffusion method. The pathogens expressed distinct growth response to the oils. As compared to colony growth of non-treated controls, all EO...

A comprehensive chemical profiling of 1,1,1,2-tetrafluoroethane (freon R134a) subcritical extracts from the main genotypes of oil-bearing roses, was performed by gas chromatography–mass spectrometry (GC/MS) and gas chromatography with flame ionization detection (GC-FID) in order to reveal the differences in their chemical composition. One hundred a...

Subcritical extraction of Rosa damascena Mill. was performed with Freon 143a. The pressure and duration of the process were studied to obtain the yield. It was found that a short-time triple extraction at low pressures of 5-6 bar resulted in the highest yield – 0.151%. The chemical composition of the product revealed a high scent content: β-phenyle...

Bulgaria is known as one of the main producers of high quality rose oil from Rosa damascena Mill. and Rosa alba L., valued on the world market for fragrances with the rating "Royal Class". Wastewater is one of the wastes discarded in the environment as result of water steam distillation applied in rose oil production. Does this waste harm the soil...

Background: The specific chemotherapeutics against herpes simplex virus type 1 (HSV) are nucleoside analogues such as acyclovir (ACV), but the most important problem is the formation of resistant mutants. The search for new therapeutic alternatives leads us to the purpose of investigating the effects of Rosa damascena Mill. and Rosa alba L. essent...

Bulgarian Rosa alba L. essential oil is widely used in perfumery, cosmetics and pharmacy. The scarce data about its cytotoxic/genotoxic effect and anti-cytotoxic/anti-genotoxic potential gave us a reason to set our aim: i) to study its cytotoxic/genotoxic activities, iii) to explore its cytoprotective/genoprotective potential against the experiment...

Distillation wastewater, by-products from steam and water distillation as well as raw material used as control of flower heads of Helichrysum italicum were comparative analyzed for content of the biologically active compounds by GC/MS. Acetone exudates, methanol extracts and ethyl acetate fractions obtained after alkaline hydrolyze of the studied ma...

The present study was undertaken to test the inhibitory activity of several plant extracts against bacteria causing economically important diseases on fruit and nut trees. Essential oils derived from oregano (Origanum vulgare ssp. hirtum), common sage (Salvia officinalis), savory (Satureja pilosa), monarda (Monarda didyma) and an extract from clove...

Plants from the Rosacea family are rich in natural molecules with beneficial biological properties, and they are widely appreciated and used in the food industry, perfumery, and cosmetics. In this review, we are considering Rosa damascena Mill., Rosa alba L., Rosa centifolia L., and Rosa gallica L. as raw materials important for producing commercia...

Rosa x damascena Mill. also known as the oil-bearing rose is the predominant rose species worldwide used for production of rose essential oil products applied in the perfumery, cosmetics, pharmaceutical and food industries. The present chapter presents detailed information covering diverse aspects related to the botany, economic use and breeding st...

Rosa x damascena Mill. also known as the oil-bearing rose is the predominant rose species worldwide used for production of rose essential oil products applied in the perfumery, cosmetics, pharmaceutical and food industries. The present chapter presents detailed information covering diverse aspects related to the botany, economic use and breeding st...

The chemical composition and aroma profile of seven Rosa damascena Mill. essential oil samples from Saudi Arabia (Al-Shafa, Al-Hada and Al-Taif area) were studied in details by means of gas chromatography-mass spectrometry (GC/MS) and gas chromatography with flame ionization detection (GC-FID). As a result, more than 120 compounds, containing C3-C2...

Organic production and markets are expanding rapidly. A field study was carried to compare effects of different agriculture soil system on productivity of Rosa damascena Mill. and Rosa damascena x Rosa gallica. The experiment was conducted on six private arable areas with oil bearing rose in Rose valley, Southern Bulgaria. The selected study area s...

This study assessed phytochemical profile and antioxidant capacity of hydrosols, obtained by water-steam distillation of Rosa alba L. and Rosa damascena Mill. plants, grown in experimental field of the Institute of Rose and Essential Oil Plants, Bulgaria. The identification of hydrosols' compounds was performed using 7890A/5975 GC-MS system, the to...

Genotoxic activities of geraniol and its anti-cytotoxic/anti-genotoxic potential against genotoxin N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were studied in two types of test-systems using test for induction of chromosome aberrations. The phytochemical possesses a clearly expressed defense potential against MNNG. The anti-cytotoxic/anti-genotoxic...

Genotoxic activities of geraniol and its anti-cytotoxic/anti-genotoxic potential against genotoxin N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were studied in two types of test-systems using test for induction of chromosome aberrations. The phytochemical possesses a clearly expressed defense potential against MNNG. The anti-cytotoxic/anti-genotoxic...

Geraniol is a common component of essential oils of various plant species and is widely used in cosmetics, per-fumery and household products. In the present study, we investigated the cytotoxic, genotoxic and antioxidant activities of different concentrations of the monoterpenoid geraniol and its anti-cytotoxic and anti-genotoxic potential against...

Rosa alba L., also known as the white oil-bearing rose, has been cultivated in relatively small areas of Europe – predominantly in the Rose Valley of Bulgaria. An increasing number of studies in vitro and in vivo, including clinical studies, have demonstrated the therapeutic efficacy of rose oils in recent years. However, little is known about the...

Commercial batches of pepper fruits, a variety of the long red fleshy pepper with serious deformities were established in the period 2014-2016. Analyses of spotted and deformed fruits purchased from supermarkets in the summer months were carried out by the serological method ELISA, variant DAS-ELISA and artificial inoculation of test (indicator) pl...

Geraniol is an acyclic monoterpene alcohol with characteristic rose-like odour. It is an important constituent of Bulgarian Rosa alba L. and Rosa damascena Mill. essential oils. The purpose of the present study was to investigate antioxidant ability as well to reveal the potential for antiviral activity of geraniol against the replication of viruse...

Essential oils from Rosaceae family are a challenge for scientists because of their wide range of pharmacological activities. The purpose of the present work was to determine chemical composition and to compare antiradical and antifungal activities of 6 essential oils from Rosa genus growing in Bulgaria, Moldova and China, and some of their ingredi...

Essential oil from Rosa alba L. - white oil-bearing rose, is produced quite recently only in Bulgaria. Because a few documentation of its phytochemical screening and biological activities, the purpose of this work was to investigate chemical composition, antiradical properties and antimicrobial activity of Rosa alba L. essential oil against oral pa...

Garden sage (Salvia officinalis L.) is a medicinal, culinary, ornamental, and essential oil plant with a wide range of ecological adaptation. Garden sage essential oil traditionally is extracted by steam distillation from the above-ground biomass and has widespread applications as an aromatic agent in the food and pharmaceutical industries as well...

Antimicrobial activity of two trade lots of essential oils from Rosa alba L. against Gram-positive bacteria, belonging to genera Staphylococcus and Bacillus, Gram-negative bacteria, belonging to genera Escherichia, Pseudomonas, Salmonella and Citrobacter and yeasts, belonging to genera Candida was investigated. It was determined that Gram-positive...

Essential oils from Rosaceae family are a challenge for scientists because of their wide range of pharmacological activities. The purpose of the present work was to determine chemical composition and to compare antiradical and antifungal activities of 6 essential oils from Rosa genus growing in Bulgaria, Moldova and China, and some of their ingredi...

It was monitored the daily dynamics of components in the natural essential oil of Rosa damascena Mill. flowers using static headspace solid phase microextraction technique (sHS-SPME). 37 compounds were identified. It was established that the headspace model was a combination of terpene alcohols: geraniol (0.57 - 32.33 %), citronellol (1.18 - 30.93...

The antifungal activities of peppermint essential oils obtained from standard cul-tivar, two newly selected Mentha x piperita L. and allopolyploid hybrid of Mentha x piperita L. x Mentha arvensis, growing in Bulgaria, were investigated against 15 clinical isolates of Candida albicans (5 oropharyngeal, 5 vaginal and 5 skin damaging strains) and C. a...

The chemical composition of historical rose oil (produced in 1944) from Bulgaria was investigated by GC and GC-MS. The rose oil was found to be rich in citronellol (23.4 %), geraniol (19.0 %), nonadecane (11.9 %), and nerol (7.5 %). In general, the chemical composition of this historical rose oil corresponded exactly to the criteria stated in the c...

To improve the comparatively low oil yield, pulsed electric fields have been applied on fresh rose blossoms with an energy input of 10 kJ/kg as well as 20 kJ/kg. It was reported a rise in yield of 2- 46 %, additionally the distillation time could be reduced. An increase of ratio terpene alcohols/hydrocarbones from 33 % to 285 % was observed in trea...

Export Date: 18 October 2014

Gas chromatography and gas chromatography-mass spectrometry was applied for comparison the chemical profile of the essential from three genotypes of oil-bearing roses: Rosa damascena Mill., Rosa alba L. and Rosa No. 836/61 (Rosa damascena Mill. x Rosa gallica L.). The analyses identified 60 components. This study reveals that they have the same qua...

The essential oil content of the oil-bearing rose (Rosa damascene Mill.) is relatively low, around 0.3–0.4mLkg−1 in fresh flowers. There is a need to increase essential oil yield of oil-bearing rose. The objective was to examine the effect of Tween 20 (polyoxyethylene sorbitan monolaurate) applied with, or without, maceration of flowers on oil cont...

Besides Rosa damascena Mill., the white oil-bearing rose species Rosa alba L. is of importance for the Bulgarian rose production. Their blossoms essential oil content (0.015 - 0.030%) is comparably low and results in a low yield and an expensive end product. To increase oil yield pulsed electric fields (PEF) with an energy input of 10 kj/kg as well...

NEDKOV, N., A. DOBREVA, N. KOVACHEVA, V. BARDAROV and A. VELCHEVA, 2009. Bulgarian Rose Oil of White Oil-bearing Rose. Bulg. J. Agric. Sci., 15: 318-322 The white oil-bearing rose (R. alba L.) is the second most important plant for Bulgarian rose production. Regardless of the growing interest to this plant worldwide, its essential oil has not been...

In the present study we evaluated the composition and antimicrobial activity of various rose oils from Bulgaria, Turkey, Morocco, Iran and China against three Gram-positive, three Gram-negative bacteria and two yeasts. The composition of the studied essential oils was determined by GC and GC/MS. Citronellol was the major compound of all the oil sam...