Amany AdelAnimal Health Research Institute · reference laboratory for quality control of poultry production
Amany Adel
PhD of Virology
About
73
Publications
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173
Citations
Introduction
Additional affiliations
July 2007 - present
Publications
Publications (73)
A variety of illnesses, including arthritis, tenosynovitis, stunted growth, and malabsorption syndrome, are caused by Avian Reoviruses (ARVs), which have become more prevalent in Egypt during recent years and resulted in significant economic losses. This study investigated 27 suspected samples collected from 14 broiler breeders and 13 broilers suff...
Citation: AbdelRahman et al., Application of PCR-based serogrouping of Salmonella enterica serotypes isolated from the poultry in Egypt. SVU-IJVS 2024, 7(1): 62-70. Abstract Salmonella spp. remains a significant concern in poultry flocks due to its potential impact on both animal health and public health. In this study, a comprehensive approach to...
The cytokine storm induced by duck hepatitis A virus type 1 (DHAV-1) infection significantly contributes to severe, rapid deaths and economic losses in the duck industry in Egypt. This study aimed to investigate the potential inhibitory effect of a nanoemulsion containing turmeric and black pepper oil on the immune response and pathogenesis of DHAV...
Infectious bursal disease viruses (IBDVs) have a profound impact on poultry production worldwide, directly causing mortality rates of up to 100%, and indirectly through their immunosuppressive effects. Since the emergence of the antigenically modified very virulent IBDV (vvIBDV) in Egypt in late 1999, the country has experienced recurrent outbreaks...
Background and aim:
Biosecurity implementation is fundamental to combating diseases and antibiotic resistance. Therefore, this study aimed to examine the correlation between the implementation of biosecurity measures in small-scale duck farms and the incidence of infectious diseases that threaten the duck industry.
Materials and methods:
Twenty...
Late in 2016, multiple reassortant highly pathogenic (HP) avian influenza virus (AIVs) H5N8 was detected. AIVs infect different isolated hosts with a specific viral tropism. In the current study, the whole genome of the Egyptian A/chicken/NZ/2022 was genetically characterized. The H5N8-A/Common-coot/Egypt/CA285/2016, A/duck/Egypt/SS19/2017 previous...
Identification of avian infectious bronchitis virus (IBV) genotypes is essential for controlling infectious bronchitis (IB) disease, because vaccines that differ from the circulating strains might not provide efficient cross-protection. In Egypt, IBV strain typing is a difficult process, due to the widespread distribution of four genotype lineages...
The highly pathogenic avian influenza (HPAI) H5N8 virus was first detected in Egypt in late 2016. Since then, the virus has spread rapidly among different poultry sectors, becoming the dominant HPAI H5 subtype reported in Egypt. Different genotypes of the HPAI H5N8 virus were reported in Egypt; however, the geographic patterns and molecular evoluti...
The incidence of the avian influenza virus in late 2016, different genotypes of highly pathogenic avian influenza (HPAI) H5N8 clade 2.3.4.4b have been reported among different domestic and wild bird species. The virus became endemic in the poultry population, causing a considerable economic loss for the poultry industry. This study screened five os...
1. This paper details the establishment of a diagnostic system based on the real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid, simple and sensitive detection of genotype VII of Newcastle disease virus (NDV) directly from field samples. One specific set of six primers were designed which targeted th...
Since it was first discovered, the low pathogenic avian influenza (LPAI) H9N2 subtype has established linages infecting the poultry population globally and has become one of the most prevalent influenza subtypes in domestic poultry. Several different variants and genotypes of LPAI H9N2 viruses have been reported in Egypt, but little is known about...
Inclusion body hepatitis is an economically significant viral disease that primarily affects broiler and chicken breeders. Inclusion body hepatitis was caused by at least 12 different Aviadenovirus serotypes. The purpose of this study was to use PCR and phylogenetic analysis to characterize fowl adenovirus isolates that were circulating from 2019 t...
Poultry health problems of chickens are usually accompanied by bacterial systemic infections causing severe losses in flocks, sick birds were systemically could be infected by several bacteria regardless of the clinical signs. 16s RNA sequence is a modern approach for the diagnosis of bacterial infection, especially of multiple causative agents. Te...
Bacillus cereus is one of the opportunistic foodborne bacteria producing food poisoning producing severe economic loss and representing a high risk to the human. The contamination of poultry feed with Bacillus cereus produces gastric damage and facilitates other bacterial infections. For these reasons, fresh fecal matters were collected from poultr...
In late 2016, Egypt faced multiple introductions of highly pathogenic avian influenza (HPAI) viruses of the H5N8 subtype. In a previous study, three distinct genotypes, namely, A/common-coot/Egypt/CA285/2016 (H5N8) (CA285), A/duck/Egypt/SS19/2017 (H5N8) (SS19), and A/duck/Egypt/F446/2017 (H5N8) (F446), during the first wave of infection, were isola...
Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsible for inclusion body hepatitis (IBH), gizzard erosion disease (GED), and hepatitis/hydropericardium syndrome (HHS). This study presents a comprehensive overview of FAdVs in Egypt, with a focus on the epidemiological features of virus serotypes across t...
Hepatitis-hydropericardium syndrome (HHS) is an acute infectious disease caused by fowl adenovirus serotype-4 (FAdV-4), which mainly infects broilers aged 4-5 weeks. During winter, definitely in January 2021, a 32-day-old broiler flock (Cobb-500), in Alexandria Governorate, suffered from unusually high mortality. The infected chickens showed up a d...
Hepatitis hydropericardium syndrome (HHS) is an acute infectious disease caused by fowl adenovirus serotype-4 (FAdV-4), which mainly affects broilers aged 4-5 wk. During the winter of January 2021, a 32-day-old broiler flock (Cobb-500) suffered from unusually high mortality (15%) in the Alexandria Governorate, Egypt. The chickens showed depression,...
Despite the low pathogenicity of the H9N2 avian influenza viruses, they can induce severe economic losses in various poultry sectors in conjunction with other factors. In Egypt, low-pathogenic avian influenza (LPAI) H9N2 became endemic in 2011 and has undergone continuous genetic evolution since then. The regular monitoring of the evolution of the...
In late 2016, Egypt was subjected to multiple introductions of reassorted highly pathogenic avian influenza viruses (HPAI) subtype H5N8. In a previous study, we reported three distinct genotypes during the first wave of infection represented by CAO285, SS19, and F446 viruses that were isolated from wild birds, backyard, and a commercial farm, respe...
Despite the low pathogenicity of the H9N2 avian influenza viruses, they can induce severe economic losses in various poultry sectors in conjunction with other factors. In Egypt, low-pathogenic avian influenza (LPAI) H9N2 became endemic in 2011 and has undergone continuous genetic evolution since then. The regular monitoring of the evolution of the...
Low pathogenic avian influenza (LPAI) virus of subtype H9N2 is the most frequently
detected subtype among domestic poultry and is a public health concern because
of its zoonotic potential. Due to the multiple and complex routes of LPAIV H9N2
between geographic regions, little is known about the spatial diffusion of H9N2 virus
to, within, and from E...
Severe outbreaks of Infectious bursal disease virus (IBDV) were reported in Egypt despite vaccination. Therefore, this study was conducted to characterize infectious bursal disease (IBD) viruses circulating in Egypt during the period of 2017 - 2018. Sixteen pooled bursal tissue samples were collected from IBD suspected (9 Egyptian balady and 7 broi...
Since the first isolation of the H9N2 low pathogenic avian influenza virus in 2011, the virus has distributed rapidly and widely in different poultry sectors in Egypt causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, 1026 confirmed posit...
Since the introduction of H9N2 low pathogenic avian influenza virus in Egypt, it became an endemic disease causing considerable economic losses in different poultry sectors especially in the presence of other secondary bacterial and viral infections. The H9N2 viruses in Egypt are in continuous evolution that needs deep analysis for their evolution...
Chicken anemia virus (CAV) is an important pathogen associated with immunosuppression in chicken. In this study, out of samples collected from 115 commercial poultry farms, 12 samples were CAV positive by PCR. Partial sequence and phylogenetic analysis of VP1 gene revealed that the detected viruses were clustered to genotype I (n = 3) and genotype...
The LPAI viruses of H9N2 subtype became widely distributed in Middle Eastern countries, causing great economic losses in poultry industry especially when complicated with other pathogens. The H9N2 viruses in Egypt have a wide spread nature since its first occurrence in 2011. In this study, we collected cloacal and tracheal samples from 19 flocks fo...
The suspected presence of avian influenza virus subtype H9N2 in poultry in Egypt is a major concern since this subtype is widely distributed in different countries in the Middle East, here we describe the full genetic characterization of an avian influenza A virus (Qa/Egypt/11; H9N2) of subtype H9N2 that was previously isolated from a clinically no...
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Assembly Method :: BioEdit v. 7 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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On Apr 30, 2015 this sequence version replaced gi:385198479. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
On Apr 30, 2015 this sequence version replaced gi:385198467. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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On Mar 27, 2012 this sequence version replaced gi:375313934.
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
GenBank Accession Numbers JN828567-JN828574 represent sequences from the 8 segments of Influenza A virus (A/quail/Egypt/113413v/2011(H9N2)).
Questions
Questions (4)
I have a confusion in the results of some sanger sequence reactions for IBDV viruses.
the sequence reactions fail to give any reads or give non specific, bad quality and short fragments, although the PCR of those reactions are strongly positive and the sequenced samples was positive for both classic and vvIBDV by real time PCR.
could this failure be related to the mixed infection in samples or quasispecies phenomena?
I am in bad need to find out the scientific causes of these results supported with scientific published papers.
thanks for help
hi , if you please i need to know what are the reasons of the false positive results in real time RT-PCR of non specific template reaction, the primers are not similar to the non specific template while the probe is close similar to the non specific template? could i get an explanation ?
Definitely, my problem is in the reactions that have a DNA but it is supposed to be non-specific for the primers and probe. After checking the primers and probe sequence, I found that the primers were completely non-specific for this DNA but the probe was specific partially. so, my question here is, can the reaction be falsely positive because of the probe?
thank you
Please i need research articles about the role of pigeon in evolution of the LPAI H9N2
I converted files with fasta format to nexus format by using MEGA 7 software, but the converted nexus format did not work in the BEAST v2.4.5 software
