Alla Tereshchenko

Odessa National University · Department of Experimental Physics

This review article highlights the application of beneficial physico-chemical properties of ZnO nanostructures for the detection of wide range of biological compounds. As the medical diagnostics require accurate, fast and inexpensive biosensors, the advantages inherent optical methods of detection are considered. The crucial points of the immobiliz...

Novel sensitive optical biosensor for the determination of Grapevine virus A-type (GVA) proteins (GVA-antigens) has been designed. This biosensor was based on thin films of Zinc oxide (ZnO) deposited by atomic layer deposition (ALD). The ZnO-based films have demonstrated favorable surface-structural properties for the direct immobilization of antib...

In this research a mechanism of interaction between semiconducting TiO2 layer and bovine leukemia virus proteins gp51, applied in the design of photoluminescence-based immunosensors, is proposed and discussed. Protein gp51 was adsorbed on the surface of nanostructured TiO2 thin film, formed on the glass substrates (TiO2/glass). Photoluminescence (P...

In this research a whispering gallery mode (WGM) resonator based on vertically oriented ZnO nanorods, which were formed on silicon surface (silicon/ZnO-NRs), has been applied in the design of optical immunosensor that was dedicated for the determination of grapevine virus A-type (GVA) proteins. Vertically oriented ZnO-NRs were grown on silicon subs...

Quality control of food and agriculture production is an inseparable part of human safety and well-being. Salmonella infections belong to one of the most monitored pathogens in the world, therefore advanced determination of this pathogen can decrease the risks of human diseases caused by this microorganism. In this research we introduce a novel opt...

ZnO-based nanostructured thin films are broadly applied as a template for biosensors due to their fundamental physico-chemical properties. Optical properties of ZnO thin layers, in particular, an intense room temperature photoluminescence open a great possibility to be used for optical biosensor, in particular, immunosensor applications. Due to the...

ZnO-based nanostructured thin films are broadly applied as a template for biosensors due to their fundamental physico-chemical properties such as the direct wide band gap semiconductor (Eg ∼3.37eV) complemented by high surface to volume ratio of its nanostructures that has a high isoelectric point. Good biocompatibility and affinity towards biomate...

Optical properties of ZnO, in particular, an intense room temperature photoluminescence open great possibilities to for application in optical biosensors. In this type of sensors, the changes in the photoluminescence signal of nanostructured material are used for the determination of analyte and its amount by the characterization of the photolumine...

Interaction between nanostructured semiconductor thin layers and immune complex based proteins is a fundamental issue of the formation of nanobiointerface in the various biosensors, in particular, optical biosensors. In this work, the main aspects of the interaction between photoluminescent TiO2 nanoparticles and Bovine leucosis virus (BLV) protein...

The thin films based on ZnO nanostructures have been intensively used as a template for biosensors due to their fundamental physico-chemical properties as a direct wide band gap semiconductor (Eg 3.37 eV), complemented by a high surface-to -volume ratio of its nanostructures and chemically stable material. ZnO has a high isoelectric point (pH 9-9.5...

A pencil graphite electrode was used for electrochemical synthesis of polypyrrole in an aqueous solution. Two key features of the polymerization were investigated: 1) the pH of the aqueous solution and 2) number of potential pulses for obtaining the polymer on the electrode. The pH values of the aqueous solutions were as follows: pH 1.0, pH 3.0 and...

A model of interaction between photoluminescent TiO2 nanoparticles and Bovine Leucosis proteins gp51 during the formation of optical immunosensor for the determination of gp51 antibodies has been proposed. The main reason of the changes in the photoluminescence (PL) spectra (i.e. PL maxima shifts and PL intensity variations) as a result of Bovine L...

In this research we have applied sol-gel synthesis for the deposition of tungsten (VI) oxide (WO3) layers using two different reductants (ethanol and propanol) and applying different dipping times. WO3 samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR), photolumi...

Immunosensors based on hybrid metal oxide nanostructures (TiO2, ZnO, WO3, etc.) are broadly applied in various fields of human life -- from medical diagnostics up to national security and defense. Immunosensors are based on a specific interaction between the antibody--antigen couple which has high specificity and sensitivity to detect analytes. Opt...

The modelling of protein-protein binding kinetics is important for the development of affinity-sensors and the prediction of signaling protein based drug efficiency. Therefore, in this research we have evaluated the binding kinetics of several genetically designed protein models: (i) three different ligands based on granulocyte colony-stimulating f...

Bovine leucosis is a lethal cancerous disease caused by Bovine leukemia virus (BLV) that belongs to one of the most monitored family of retro-viruses in the world. There is a significant risk that BLV can infect other mammalians like it was in the case of Human immunodeficit virus (HIV). Due to lethal epidemical nature, the outbreaks of Bovine leuc...

In this research we report the gas-sensing properties of TiO2-x/TiO2-based hetero-structure, which was ‘self-heated’ by current that at constant potential passed through the structure. Amperometric measurements were applied for the evaluation of sensor response towards ethanol, methanol, n-propanol and acetone gases/vapours. The sensitivity towards...

In this research the main aspects of the interaction mechanism between nanostructured TiO2 layer and BLV proteins gp51 have been investigated during the formation of photoluminescence-based immunosensor. Bovine leucosis protein gp51 was adsorbed on the surface of a nanostructured TiO2 thin film, formed on glass substrates. A photoluminescence (PL)...

Bovine leucosis is a lethal cancerous disease caused by Bovine leukemia virus (BLV) that belongs to one of the most monitored family of retro-viruses in the world. There is a significant risk that BLV can infect other mammalians like it was in the case of Human immunodeficit virus (HIV). Due to lethal epidemical nature, the outbreaks of Bovine leuc...

In this research, a 9,10-phenanthrenequinone (PQ) was electrochemically polymerized on a graphite rod electrode using potential cycling. The electrode modified by poly-9,10-phenanthrenequinone (poly-PQ) was studied by means of cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy and scanning electron microscopy. The p...

Optical, structural and adsorptional properties of ZnO nanostructured thin films have been studied for the development of highly sensitive optical immunosensor. Photoluminescent properties of ZnO thin films, deposited by atomic layer deposition (ALD) on the silicon substrates were applied for determination of Grapevine virus A-type proteins (GVA-an...

The structural, surface and optical properties of ZnO and TiO2 nanostructured thin films have been investigated for the development of optical immunosensors [1,2]. Novel sensitive optical immunosensor based on ZnO thin films for determination of Grapevine virus A-type proteins (GVA-antigens) has been designed [1].The immobilization of anti-GVA anti...

The work is focused on technology and characterization issues of silicon pillar nanostructures in combination with metal oxides, such as ZnO and TiO 2 , for various applications in field of biosensor and solar energy. The metal-assisted chemical etching method (MACE) modified with latex nanobeads lithography and spin-coating technique, was used to...

The thesis is devoted to the study of the influence of bio-molecules adsorption on the surface of Titanium dioxide (TiO2) nanostructured thin films, as well as to the establishment of the main aspects of interaction between biomolecules and TiO2. The method of the forming a bio-sensitive layer on the surface of nanostructured TiO2 films has been de...

In this report an application of some metal oxide nanostructures as a biosensor platform for the detection of dangerous biological compounds (Bovine leucosis, Salmonella) have been discussed. The attention is paid to the TiO2 nanoparticles and ZnO nanorods deposited on the flat surface. The changes in photoluminescense signal from nanostructured su...

A novel optical sensor based on TiO2 nanoparticles for Valine detection has been developed. In the presented work, commercial TiO2 nanoparticles (Sigma Aldrich, particle size 32 nm) were used as sensor templates. The sensitive layer was formed by a porphyrin coating on a TiO2 nanostructured surface. As a result, an amorphous layer between the TiO2...

Novel immune photoluminescent biosensor, based on TiO2 nanoparticles, for retroviral leucosis detection has been developed. The photoluminescence spectra were excited by solid state laser with wavelength 355 nm and measured in the range of 370-800 nm. Original photoluminescence spectrum of TiO2 nanoparticles showed wide maximum at 515 nm. The biose...

A novel method for Salmonella detection using photoluminescence immune biosensor, based on TiO2 nanowires, is proposed. TiO2 nanowires (NWs) were solved in ethanol and then deposited on glass substrates. To fabricate biosenstitive layer, salmonella antibodies (Ab) were immobilized on the surface of TiO2 NWs. Photoluminescence (PL) spectra of titani...

A novel method for Salmonella detection using photoluminescence immune biosensor, based on TiO2 nanowires, is proposed. TiO2 nanowires (NWs) were solved in ethanol and then deposited on glass substrates. To fabricate biosenstitive layer, salmonella antibodies (Ab) were immobilized on the surface of TiO2 NWs. Photoluminescence (PL) spectra of titani...

Salmonella antibodies, immobilized on ZnO nanostructures surface from liquid solutions, resulted in enhance of ZnO photoluminescence intensity. Biosensor reaction to salmonella antigens caused to decrease of PL intensity for all measured antigen concentrations.

The presented paper is devoted to investigation of properties of silica nanofibers hydrogels for biosensors applications. SiO2 nanofibers were prepared by the electrospinning method from polymeric solutions, containing SiO2 precursor, followed by a calcination procedure to obtain highly crystalline, pure inorganic nanofiber materials. The structura...