Alexandre Samoylov

Auburn University | AU · College of Veterinary Medicine

Objectives Phage–gonadotropin-releasing hormone (GnRH) constructs with potential contraceptive properties were generated in our previous study via selection from a phage display library using neutralizing GnRH antibodies as selection targets. In mice, these constructs invoked the production of antibodies against GnRH and suppressed serum testostero...

Overpopulation of free-roaming and wildlife animals negatively affects economy and public health in many parts of the world. Contraceptive vaccines are viewed as a valuable option for reducing numbers of unwanted animals. This study develops vaccines for potential use in animal contraception exploiting a DNA platform. Objectives of the study were t...

Phage display is based on genetic engineering of phage coat proteins resulting in fusion peptides displayed on the surface of phage particles. The technology is widely used for generation of phages with novel characteristics for numerous applications in biomedicine and far beyond. The focus of this study was on development of phage-peptide construc...

GnRH receptors play vital roles in mammalian reproduction via regulation of gonadotropin secretion, which is essential for gametogenesis and production of gonadal steroids. GnRH receptors for more than 20 mammalian species have been sequenced, including human, mouse, and dog. This study reports the molecular cloning and sequencing of GnRH receptor...

Disclosed are methods, compositions, zona pellucida binding peptides and polypeptides, and expression vectors for use in species-specific immunocontraception of animals, which include landscape bacteriophage. The disclosed compositions may include immunogenic compositions or vaccines.

A composition, system and method for modifying an olfactory response to an odorant is disclosed. In some embodiments, the composition includes crystalline metal nanoparticles dispersed in an aqueous medium. The composition isapplied to olfactory tissues using a suitable applicator or dispenser. The metal nanoparticles are believed to interact with...

A composition, system and method for modifying an olfactory response to an odorant is disclosed. In some embodiments, the composition includes crystalline metal nanoparticles dispersed in an aqueous medium. The composition is applied to olfactory tissues using a suitable applicator or dispenser. The metal nanoparticles are believed to interact with...

Gonadotropin releasing hormone (GnRH) receptors play vital roles in mammalian reproduction via regulation of gonadotropin secretion, which is essential for gametogenesis and production of gonadal steroids. GnRH receptors for over 20 mammalian species have been sequenced, including that of human, mouse and dog. This study reports the molecular cloni...

Contents Overpopulation of cats and dogs is a serious worldwide problem that demands novel, safe and cost‐effective solutions. The objective of this study was to generate and characterize phage‐peptide conjugates with gonadotropin‐releasing hormone ( G n RH ) for potential use as an immunocontraceptive. A filamentous phage vector f5‐8 with wild‐typ...

Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific properties, an important feature for w...

A composition, system and method for modifying an olfactory response to an odorant is disclosed. In some embodiments, the composition includes crystalline metal nanoparticles dispersed in an aqueous medium. The composition is applied to olfactory tissues using a suitable applicator or dispenser. The metal nanoparticles are believed to interact with...

The hypothalamic gonadotropin-releasing hormone (GnRH) plays a critical role in the regulation of reproduction. GnRH activates GnRH receptor (GnRHR), a member of the seven-transmembrane-, G-protein coupled receptor family that is mainly expressed by anterior pituitary gonadotropes. GnRHR mediates the secretion of luteinizing hormone (LH) and follic...

The focus of this study is on development of vaccines using filamentous phage as a delivery vector for immunogenic peptides. The use of phage as a carrier for immunogenic peptides provides significant benefits such as high immunogenicity, low production costs, and high stability of phage preparations. However, introduction of live recombinant phage...

Using centrifugation and filtration to recover protein aggregates comprisng metallic nanoclusters for use as apototic therapeutic in treatment of cell proliferative disorders. Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In on...

Clearing misfolded proteins from blood via incubation with proteon nucleation center (PNC) which comprise metallic nanoparticles consisting of copper, zinc and/or iron; diagnosing metabolic disorders. Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological mate...

Zona pellucida (ZP) glycoproteins play a central role in sperm-oocyte binding and fertilization. Sperm protein sequences that are involved in sperm-ZP recognition and have an important role in fertilization represent attractive targets for development of contraceptive vaccines, yet are currently unknown. To identify peptide sequences that recognize...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinafter termed "proteons" are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and...

Lysosomal beta-galactosidase is required for the degradation of GM1 ganglioside and other glycolipids and glycoproteins with a terminal galactose moiety. Deficiency of this enzyme leads to the lysosomal storage disorder, GM1 gangliosidosis, marked by severe neurodegeneration resulting in premature death. As a step towards preclinical studies for en...

Methods and compositions for identifying and characterizing one or more ligands of a peptide are provided. In particular, the invention provides a phage ligand sensor device (PLSD) comprising a sensor coupled to a binding element of interest. Binding elements of interest comprise phage displaying at least one foreign peptide. The PLSD and assays fi...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, herein aftertermed "proteons" are misfolded or partially misfolded proteins surrounding a metallic nanocluster, termed "proteon nuc...

The antimycotic activity of amphotericin B (AmB) depends on its ability to make complexes sterols to form ion channels that cause membrane leakage. To study this phenomenon, surface pressure (pi) as a function of surface area (A) and pi-A hysteresis were measured in monolayers of AmB-cholesterol mixtures on the water-air interface. The most stable...

Biosensors based on landscape phages immobilized by physical adsorption on the surface of a quartz crystal microbalance was used for detection of beta-galactosidase from Escherichia coli. The sensor had a detection limit of a few nanomoles and a response time of a approximately 100 s over the range of 0.003-210 nM. The binding dose-response curve h...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinaftertermed "proteans" are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and...

Patented signal analytic algorithms applied to hydrophobically transformed, numerical amino acid sequences have previously been used to design short, protein-targeted, L or D retro-inverso peptides. These peptides have demonstrated allosteric and/or indirect agonist effects on a variety of G-protein and tyrosine kinase coupled membrane receptors wi...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinaftertermed "proteans" are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and...

Methods and compositions for evaluating the affinity of one or more ligands of interest are provided. In particular, a ligand sensor device (LSD) comprising a sensor coupled to a peptide of interest is provided. Assays using the LSD allow detection of ligand-peptide interactions directly in tissue samples and thus provide an in vitro assay to chara...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinafter termed "proteons" are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and...

Compositions and methods for binding to Salmonella bacteria are provided. The compositions comprise peptide sequences which bind to Salmonella bacteria with high specificity. The compositions are useful in identification, detection, and isolation of Salmonella bacteria. The compositions are also useful for the delivery of a wide variety of compound...

Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinafter termed "proteons" are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and...

Methods and compositions for identifying and characterizing one or more ligands of a peptide are provided. In particular, the invention provides a phage ligand sensor device (PLSD) comprising a sensor coupled to a binding element of interest. Binding elements of interest comprise phage displaying at least one foreign peptide. The PLSD and assays fi...

The specific and selective detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir-Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated in liquid samples. These biosensors were selective to S. typhymurium in the presence of large concentrations of Escherichia coli...

Methods and compositions for evaluating the affinity of one or more ligands of interest are provided. In particular, a ligand sensor device (LSD) comprising a sensor coupled to a peptide of interest is provided. Assays using the LSD allow detection of ligand-peptide interactions directly in tissue samples and thus provide an in vitro assay to chara...

Biosensors based on phage display-derived peptides as biorecognition molecules were used for the detection of cell surface cross-species markers in tissue homogenates. The peptide selected for murine myofibers was immobilized onto the surface of an acoustic wave sensor by biotin-streptavidin coupling. To detect peptide-receptor interaction, the sen...

Screening with a 7-mer phage display peptide library, a panel of cell-targeting peptides for the murine microglial cell line, EOC 20, was recognized. A number of similar, but not identical, sets of sequences representing more than 75% of all the cell line-binding clones were identified. Comparative analysis indicated that motif S/(T) F T/(X) Y W is...

ASSLNIA, a peptide selected for murine myofibers using phage display technology, was immobilized onto an acoustic wave sensor. The sensor responded to murine and feline muscle homogenates indicating crosspieces interactions. Kidney, liver, and brain preparations produced insignificant responses.

Ion channel assembled from molecules of amphotericin B and cholesterol with a 2:1 stoichiometry were immobilized in lipid monolayers on the liquid/gas interface, liquid/solid interface, and in bilayers. Amphotericin B and cholesterol complexes assemble in two, three, four, etc. subunit aggregates to form ion channels in bilayers, and multilayers tr...

The polyene antibiotic amphotericin B (AmB) is known for both its antifungal action, and its ability to induce ion leakage in lipid membranes. In the presence of sterols (cholesterol or ergosterol), AmB forms ion permeable channels that facilitate movement of ions across the membrane. To better understand how AmB functions in lipid membranes, studi...