Alena Kubátová

Charles University in Prague | CUNI · Department of Botany

A mycotic infection manifesting as abdominal distension with free serous fluid accumulation in the coelomic cavity is documented in farmed rainbow trout. Histological examination using PAS and silver staining revealed the presence of numerous fungal hyphae in the spleen and gastrointestinal wall. The isolated fungus was sterile and identified by us...

Aspergillus section Candidi encompasses white- or yellow-sporulating species mostly isolated from indoor and cave environments, food, feed, clinical material, soil and dung. Their identification is non-trivial due to largely uniform morphology. This study aims to re-evaluate the species boundaries in the section Candidi and present an overview of a...

Novel species of fungi described in this study include those from various countries as follows: Australia , Agaricus albofoetidus , Agaricus aureoelephanti and Agaricus parviumbrus on soil, Fusarium ramsdenii from stem cankers of Araucaria cunninghamii , Keissleriella sporoboli from stem of Sporobolus natalensis , Leptosphaerulina queenslandica and...

Triadelphia (Ascomycota, Microascales) is a genus encompassing pleomorphic dematiaceous micromycetes occurring sporadically on rotting wood and other plant material, infrequently also on other substrates. In this study, we document a second record of Triadelphia morgoensis, found on a twig of Populus nigra near Prague (Czech Republic), after its or...

Novel species of fungi described in this study include those from various countries as follows: Algeria, Phaeoacremonium adelophialidum from Vitis vinifera. Antarctica, Comoclathris antarctica from soil. Australia, Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia, Eremothecium peggii in fruit of Citrus australis, Micr...

Novel species of fungi described in this study include those from various countries as follows: Algeria , Phaeoacremonium adelophialidum from Vitis vinifera . Antarctica , Comoclathris antarctica from soil. Australia , Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia , Eremothecium peggii in fruit of Citrus australis...

Abstract: Two new species, Penicillium krskae (isolated from the air as a lab contaminant in Tulln (Austria, EU)) and Penicillium silybi (isolated as an endophyte from asymptomatic milk thistle (Silybum marianum) stems from Josephine County (Oregon, USA)) are described. The new taxa are well supported by phenotypic (especially conidial ornamentatio...

Four new Keratinophyton species (Ascomycota, Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii, and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy, and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) r...

The genus Pseudogymnoascus encompasses soil psychrophilic fungi living also in caves. Some are opportunistic pathogens; nevertheless, they do not cause outbreaks. Pseudogymnoascus destructans is the causative agent of the white-nose syndrome, which is decimating cave-hibernating bats. We used comparative eco-physiology to contrast the enzymatic pot...

Four new Keratinophyton species (Ascomycota: Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) reg...

Four new Keratinophyton species (Ascomycota, Pezizomycotina, Onygenales), K. gollerae , K. lemmensii , K. straussii and K. wagneri , isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS)...

The psychrophilic (cold-loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mortality in bats. We found P. de...

Novel species of fungi described in this study include those from various countries as follows: Antarctica, Cladosporium arenosum from marine sediment sand. Argentina, Kosmimatamyces alatophylus (incl. Kosmimatamyces gen. nov.) from soil. Australia, Aspergillus banksianus, Aspergillus kumbius, Aspergillus luteorubrus, Aspergillus malvicolor and Asp...

Aspergillus section Nidulantes encompasses almost 80 homothallic and anamorphic species, mostly isolated from soil, plant material, or the indoor environment. Some species are clinically relevant or produce mycotoxins. This study reevaluated the species boundaries within several clades of section Nidulantes. Five data sets were assembled, each cont...

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bla...

A six-year-old domestic shorthair cat was presented for a subcutaneous digital nodular lesion on the right forelimb. On physical examination a similar lesion was identified on the right hindlimb. Disseminated cutaneous phaeohyphomycosis was diagnosed from histopathological changes in representative tissue biopsies and fungal culture. The isolate wa...

Novel species of fungi described in this study include those from various countries as follows: Antarctica , Apenidiella antarctica from permafrost, Cladosporium fildesense from an unidentified marine sponge. Argentina , Geastrum wrightii on humus in mixed forest. Australia , Golovinomyces glandulariae on Glandularia aristigera , Neoanungitea eucal...

A new species, Saksenaea dorisiae (Mucoromycotina, Mucorales), isolated from a water sample originating from a private well in Manastirica, Petrovac, in the Republic of Serbia (Europe), is described and illustrated. .e new taxon is well supported by multilocus phylogenetic analysis that included the internal transcribed spacer (ITS) region, domains...

Spider-pathogenic fungi are widely distributed in the world. Our review shows at least eighty six spider- and harvestman-pathogenic fungi that are currently accommodated in genera Akanthomyces Lebert, Beauveria Vuill., Clonostachys Corda, Cordyceps Fr., Engyodontium de Hoog, Gibellula Cavara, Hevansia Luangsa-ard et al., Hirsutella Pat., Hymenostil...

Spider-pathogenic fungi are widely distributed in the world. Our review shows at least eighty six spider- and harvestman-pathogenic fungi that are currently accommodated in genera Akanthomyces Lebert, Beauveria Vuill., Clonostachys Corda, Cordyceps Fr., Engyodontium de Hoog, Gibellula Cavara, Hevansia Luangsa-ard et al., Hirsutella Pat., Hymenostil...

Fusarium toxigenic microfungi have the ability to produce Fusarium mycotoxins - trichothecenes type A: T-2 toxin (T-2), HT-2 toxin (HT-2) and diacetoxyscirpenol (DAS) and trichothecenes type B: deoxynivalenol (DON), and its derivatives of 3-acetyl-DON (3-AcDON), 15-acetyl-DON (15-AcDON), nivalenol (NIV), fusarenon X (FUS X); and zearalenone (ZEA)....

A new species Saksenaea dorisiae (Mucoromycotina, Mucorales), recently isolated from a water sample originating from a private well in a rural area of Serbia (Europe), is described and illustrated. The new taxon is well supported by phylogenetic analysis of the internal transcribed spacer region (ITS), domains D1 and D2 of the 28S rRNA gene (LSU),...

Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC). Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in t...

A new species Metapochonia lutea (Ascomycota, Hypocreales) is described and illustrated. This fungus differs from the other taxa in the genus Metapochonia by its production of intensive yellow pigment in culture, conidiophores with relatively complex verticillate branching, bean-shaped conidia, and by delayed development of one-celled and prominent...

V průběhu dlouhodobého studia mikroskopických hub v osmdesáti evropských podzemních prostorách (jeskyně, opuštěné doly) byly nalezeny také mrtvá těla a kostry živocičhu žijících v tomto prostředí či nahodilých návštěvníků, některé s viditelnými nárosty mikroskopických hub. Přímá izolace, zředovací metoda a různá média byly použity pro izolaci konkr...

Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetopsina eucalypti on Eucalyptus leaf litter, Colletotrichum cobbittiense from Cordyline stricta × C. australis hybrid, Cyanodermella banksiae on Banksia ericifolia subsp. macrantha, Discosia macrozamiae on Macrozamia miquelii, Elsinoë bank...

Penicillium oxalicum is a frequently isolated fungus exhibiting a wide range of physiological activities that are of relevance in agriculture, biotechnology, food quality assessments, and medicine. Although widely studied, this fungus is usually identified on the basis of morphological characters but its taxonomy has never been systematically revis...

Aspergillus section Aspergillus (formerly the genus Eurotium) includes xerophilic species with uniseriate conidiophores, globose to subglobose vesicles, green conidia and yellow, thin walled eurotium-like ascomata with hyaline, lenticular ascospores. In the present study, a polyphasic approach using morphological characters, extrolites, physiologic...

Novel species of fungi described in this study include those from various countries as follows: Australia: Banksiophoma australiensis (incl. Banksiophoma gen. nov.) on Banksia coccinea, Davidiellomyces australiensis (incl. Davidiellomyces gen. nov.) on Cyperaceae, Didymocyrtis banksiae on Banksia sessilis var. cygnorum, Disculoides calophyllae on C...

The phylogenetic position of two Aspergillus strains isolated from Australian soil and phenotypically resembling A. unilateralis was investigated by using multigene phylogeny based on β-tubulin (benA), calmodulin (CaM), actin (act), and RNA polymerase II second largest subunit (RPB2) genes. The analysis supported their placement into a separate lin...

Highly acidic soils (pH < 3) represent an environment which might potentially offer new biotechnologically interesting fungi. Nevertheless, only little data on fungal communities in highly acidic habitats are available. Here, we focused on the diversity of cultivable filamentous microfungi in highly acidic soils (pH < 3) in the Czech Republic. Alto...

Biatriospora (Ascomycota: Pleosporales, Biatriosporaceae) is a genus with unexplored diversity and poorly known ecology. This work expands the Biatriospora taxonomic and ecological concept by describing four new species found as endophytes of woody plants in temperate forests of the Czech Republic and in tropical regions, including Amazonia. Riboso...

The first case of visceral mycotic infection due to Cephalotheca sulfurea (Cephalothecaceae, Ascomycota) is documented in farmed rainbow trout from a raceway culture system. The disease clinically manifested as a hyperaemic area in the liver of the fish, and histological examination using silver and PAS staining showed the presence of numerous foci...

Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to...

Bat White-nose Syndrome (WNS) fungus Pseudogymnoascus destructans had caused mass mortality in the North American bats. A single clone of the pathogen (Hap_1) was likely introduced in the United States while Eurasian population comprised of several haplotypes. The origin and spread of P. destructans remain enigmatic due in part to a lack of precise...

Scientific Reports 6 : Article number: 19829; 10.1038/srep19829 published online: 29 January 2016 ; updated: 20 May 2016 . This Article contains typographical errors.

Aim: The aim of the study was to control the microbial contamination of indoor air monitored monthly at the Transplant Unit of the University Hospital Olomouc from August 2010 to July 2011. Methods: The unit is equipped with a three-stage air filtration system with HEPA filters. The MAS-100 air sampler (Merck, GER) was used. Twenty locations wer...

A striking feature of white-nose syndrome, a fungal infection of hibernating bats, is the difference in infection outcome between North America and Europe. Here we show high WNS prevalence both in Europe and on the West Siberian Plain in Asia. Palearctic bat communities tolerate similar fungal loads of Pseudogymnoascus destructans infection as thei...

During mould surveys a number of Aspergillus strains were isolated from environmental air which did not fit any known species of the genus. They showed phenotypic as well as molecular genetic similarity with A. arenarius, A. arenarioides and A. peyronelii, three species without clear phylogenetic position. Multi-gene phylogenetic analysis comprisin...

Trichoderma fassatiaesp. nov. isolated from forest soil in the Czech Republic is described basedon phenotype and DNA sequence data (ITS rDNA, RPB2, and TEF1a). It belongs to theSemiorbisclade in the sectionPachybasium. Characters distinguishing it from similar species are provided.

Trichophyton onychocola is a recently described geophilic dermatophyte that has been isolated from a toenail of Czech patient with a history of onychomycosis due to T. rubrum and clinical suspicion of relapse. In this study, we report a similar case from Denmark in an otherwise healthy 56-year-old man. The patient had a history of great toenail inf...

The serious forest pathogen Heterobasidion annosum sensu lato was found during mycological exploration in caves and underground tunnels. Seventeen isolates of Heterobasidion annosum s.l. were obtained in these habitats in the Czech Republic, Slovakia, Romania and Spain during 2008–2012. Three species, H. abietinum, H. annosum sensu stricto, and H....

A strain of Biatriospora sp. CCF 4378 was tested for the production of secondary metabolites under submerged fermentation conditions. Eleven compounds were isolated from the culture broth, and the structures of these compounds were determined using HRMS, NMR and X-ray analysis. In addition to six known naphthoquinone derivatives, i.e. ascomycone A,...

The Aspergillus viridinutans complex includes morphologically similar, soil-inhabiting species. Although its species boundaries have not been fully defined, many isolates from the complex have been isolated as opportunistic human and animal pathogens. In the present study, these species were dominant in spoil sites subjected to various types of rec...

Extremely acidic soils (pH < 3) harbour poorly diversified mycobiota that are very different from less acidic habitats. During investigations of the mycobiota from several highly acidic soils in the Czech Republic and a coastal site in the Antarctic Peninsula, a group of hyaline fungal isolates was obtained. Based on phenotype and nuclear ribosomal...

White-nose syndrome (WNS) of bats has devastated bat populations in eastern North America since its discovery in 2006. WNS, caused by the fungus Pseudogymnoascus destructans, has spread quickly in North America and become one of the most severe wildlife epidemics of our time. While P. destructans is spreading rapidly in North America, nothing is kn...

Outbreak of exogenous Fusarium endophthalmitis after cataract surgery was evaluated. Twenty patients developed postoperative endophthalmitis. In 19 eyes, pars plana vitrectomy (PPV) was performed, in 14 cases (74 %) with primary intraocular lens explantation. In one case, the PPV was not performed because of poor general condition of the patient. S...

We report a case of mycotic pneumonia in a patient with acute myeloblastic leukemia. Rhizopus microsporus was identified as an agent of mucormycosis and proven by microscopy and culture. The determination of the isolate was supported by molecular methods. Combined treatment with surgery (right-sided pneumonectomy) and systemic amphotericin B and po...

Aspergillus section Aspergillus contains economically important, xerophilic fungi that are widely distributed in nature and the human environ-ment and are known for their ability to grow on substrates with low water activity. The taxa were revised based on sequence data from four loci, PCR fingerprinting, micro-and macromorphology, and physiology....

Due to increasing demand for natural sources of both polyunsaturated fatty acids (PUFAs) and beta-carotene, twenty eight Zygomycetes fungal soil isolates were screened for their potential to synthesize these biologically active compounds. Although all fungi produced C18 PUFAs, only nine strains were described to also form beta-carotene. While Actin...

Auxarthron is a genus within the Onygenales encompassing keratinophilic species with typical ascomata (gymnothecia) consisting of anastomosing network of thick-walled hyphae and small globose or oblate ascospores. No association of this genus with clinically relevant cases of human or animal infection has been reported. This paper describes the iso...

Two new and phylogenetically closely related species in Aspergillus section Fumigati are described and illustrated. Homothallic A. waksmanii was isolated from New Jersey soil (USA) and is represented by the ex-type isolate NRRL 179T (=CCF 4266T=IBT 31900T). Aspergillus marvanovae was isolated from water with high boracic acid anions content in Duko...

A current list of the published microfungal records isolated from various soils and soil related substrates are presented from the territory of the Czech and Slovak Republics (formerly Czechoslovakia). Rhizosphere, entomopathogenic, ovicidal, nematophagous, coprophilous, keratinophilic, dermatophytic, and thermoresistant fungi are also reported. Th...

A collection of 178 Aspergillus isolates, recovered from Czech patients, mostly from 2007–2011, was subjected to multilocus DNA sequence typing using the ITS region, β-tubulin, and calmodulin genes. An unusually wide spectrum of etiologic agents that included 36 species of Aspergillus is discussed in the context of recent taxonomic and clinical rep...

Extremely acidic soils (pH < 3) harbour mycobiota that are different from less acidic habitats and are dominated by a small number of fungal species. During investigation of the mycobiota of highly acidic soils in the Czech Republic and Iceland, a group of melanised fungal isolates was obtained. Based on phenotype and nuclear ribosomal DNA sequence...

Diagnosis of non-Aspergillus mould infections remains challenging despite application of a wide spectrum of non-culture-based microbiological techniques. Invasive diagnostic procedures are often essential. Here we present the case of 4 years old girl diagnosed with Coprinellus domesticus (anamorph Hormographiella verticillata) pneumonia during indu...

During a study of endophytic and saprotrophic fungi in the sapwood and phloem of broadleaf trees (Salix alba, Quercus robur, Ulmus laevis, Alnus glutinosa, Betula pendula) fungi belonging to an anamorphic coelomycetous genus not attributable to a described taxon were detected and isolated in pure culture. The new genus, Liberomyces, with two specie...

A. Kubátová, O. Koukol, A. Nováková (2011): Geomyces destructans, phenotypic features of some Czech isolates. – Czech Mycol. 63(1): 65–75. The microscopic fungus Geomyces destructans is a psychrophilic dermatophyte causing since 2006 a serious bat disease in North America called white-nose syndrome (WNS). In Europe, G. destruc-tans has also been re...

Introduction: Toxigenic microfungi are microorganisms capable of producing mycotoxins. They are important factors that may have a potentially negative effect on human health. The most important toxigenic microfungi are the producers of aflatoxins (AFs) and ochratoxin A (OTA). Foodstuffs are suitable substrates for the contamination, growth and prop...

Tolypocladium inflatum is known primarily for its production of the cyclosporines that are used as an immunosuppressive drug. However, we report here the production of the carcinogenic fumonisins B(2) and B(4) by this biotechnologically relevant fungal genus. These mycotoxins were detected in 11 strains tested from three species: Tolypocladium infl...

Material examined for Geomyces destructans presence found in the Czech Republic and Slovakia, host species, localities, direct microscopic examination, sequence Accession Numbers, and isolate numbers. (0.13 MB PDF)

White-nose syndrome is a disease of hibernating insectivorous bats associated with the fungus Geomyces destructans. It first appeared in North America in 2006, where over a million bats died since then. In Europe, G. destructans was first identified in France in 2009. Its distribution, infection dynamics, and effects on hibernating bats in Europe a...

A unique project exists in the Czech Republic, namely the Czech National Programme of Protection of Genetic Resources of Economically Significant Microorganisms and Tiny Animals (NPPGR), which includes nineteen Czech collections of microorganisms (bacteria, fungi), viruses, and tiny animals. It is fully financed by the Ministry of Agriculture of th...

During 2003–2005, the diversity of culturable filamentous soil microfungi in saline and acidic soils of the Soos National Natural Reserve (Czech Republic) was studied. Altogether, 28 soil samples were collected from four sampling sites and were processed by various approaches. In total, 92 fungal taxa were identified using classical and molecular m...

Geosmithia spp. (Ascomycota: Hypocreales) are little-studied, dry-spored fungi that occur in galleries built by many phloeophagous bark beetles. This study mapped the distribution and environmental preferences of Geosmithia species occurring in galleries of temperate European bark beetles. One hundred seven host tree samples of 16 tree species infe...

Introduction: Toxigenic microfungi are microorganisms capable of producing mycotoxins. They are important factors that may have a potentially negative effect on human health. The most important toxigenic microfungi are the producers of aflatoxins and ochratoxin A. Foodstuffs are suitable substrates for the contamination, growth and propagation of t...

Geosmithia spp. (Ascomycota: Hypocreales) are little-studied, dry-spored fungi that occur in galleries built by many phloeophagous bark beetles. This study mapped the distribution and environmental preferences of Geosmithia species occurring in galleries of temperate European bark beetles. One hundred seven host tree samples of 16 tree species infe...

2007): Mycobiota of wine grapes and occurrence of ochratoxin A and Alternaria myco-toxins in fresh grape juice, must and wine in the Czech Republic. – Czech Mycol. 59(2): 241–254. The aim of this study was to monitor the mycobiota of wine grapes, occurrence of ochratoxigenic microfungi in wine grapes and occurrence of ochratoxin A and Alternaria my...

Ochratoxin A is a mycotoxin produced by Aspergillus and Penicillium species. This mycotoxin is a common contaminant of various foods including cereal products, spices, dried vine fruits, coffee, cocoa, beer and wine. Apart from cereal products, beer and wine contribute significantly to ochratoxin exposure of humans. In the Mediterranean region of E...

The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45 strains (94 %) out of 48 survived both 2-d and 1-y...

Kubátová A. (2006): Chaetomium in the Czech Republic and notes to three new records. – Czech Mycol. 58(3–4): 155–171. Chaetomium (Ascomycota, Sordariales, Chaetomiaceae) is a species-rich genus, with about 100 currently accepted species. Data on the occurrence of Chaetomium species in the Czech Republic were not yet summarised; this paper is the fi...

Contamination of the air by fungi at the Transplant Intensive Care Unit (ICU) of the University Hospital in Hradec Kr·lovÈ was investigated in 2004. Air samples were taken from the patient’s breathing zone in the single rooms, in the room used for drug preparation and from the air outside the hospital building. Air was sampled with Biotest RCS Plus...

The basic overview of microorganisms - photomicrograph on-line gallery for educational purposes. Bacteria, yests and microscopic filamentous fungi in pictures. https://is.muni.cz/do/rect/el/estud/prif/ps06/mikroorg/web/index.html

All hypocrealean species of the genus Geosmithia are anamorphic fungi with connections to bark beetles. G. fassatiae, G. langdonii and G. obscura are described as new sympatric species associated with Scolytus carpini, S. intricatus and S. rugulosus in Central Europe. The species represent a complex of three sister taxa with affinities to G. flava...

As a response to project "WINE-OCHRA RISK" a pilot study in The Czech Republic was prepared. The aim of study was a monitoring of the mycobiota of wine grapes and a monitoring of toxigenic microfungi producers of ochratoxin A and selected Alternaria mycotoxins in wine grapes from domestic crop in the year 2004. Five vineyards with twenty two grape...

In the period 2001–2004, several hundreds of underground shelters (mainly abandoned galleries, caves, and cellars) inWand SW Bohemia (Czech Republic) were explored for insect cadavers with visible fungal growth. At 27 localities, 94 infected cadavers of six insect taxa were collected. The most frequent infected insects were Triphosa dubitata, Scoli...

Phytopathogenic effect of Geosmithia pallida, G. langdonii, Ophiostoma grandicarpum, O. querci, two isolates of O. piceae, and two isolates of Fusarium solani was compared using plant growth test (stem and root length of garden cress plants seeded on mycelium-covered potato carrot agar); Ophiostoma spp. and F. solani were isolated from oak, Geosmit...

Fungi cause deterioration of stored crops and may contaminate them with mycotoxins and allergens. Although fungi-rodent associations are known from nature, there is little data from the environment of stored plant products. Therefore, the goal of this work was to identify the fungi connected with the occurrence of mouse feces in stored grain in the...