Alba Estela Jofre-Garfias

UNAM · ENES-Leon

Strawberries are one of the most consumed fruits worldwide. Scientific research has revealed unique nutraceutical properties, mainly steaming from their content of polyphenols and fructooligosaccharides (FOS), well known prebiotics. Here, we report the fingerprint (chemical composition) of strawberries as molecular markers of geographical origin, g...

The growing increase in global strawberry production and consumption has been spectacular during this century. In 2019, 396,401 ha were planted, and 8.9 million tons of fruit were produced globally, and more than 50% of that volume was in the subtropical climate. The problems and losses caused by diseases and pests are of global importance, particu...

In vitro plant organogenesis requires plant regulator cytokinins to be exogenously supplied to the culture media. Cytokinins are either obtained from natural sources, from purified commercial plant extracts or by chemical synthesis. Besides plants, several species of plant pathogenic bacteria also naturally produce cytokinins. For example, Pseudomo...

Common bean Phaseolus vulgaris L. has been shown to be a recalcitrant plant to induce somatic embryo- genesis (SE) under in vitro conditions. We used an alternative strategy to induce SE in common bean based upon the use of a cytokinin (BAP) coupled with osmotic stress adaptation in- stead of SE response that is induced by auxins. Explants derived...

Root and crown rot produced by Fusarium oxysporum and by a viral complex consisting of at least five viruses has been affecting the region of Irapuato, Guanajuato, Mexico, causing serious damage to the strawberry production. For this reason, 14 years ago two Mexican organizations, INIFAP (the National Institute for Forestry, Agriculture, and Livest...

The use of macrotunnels is becoming important for the production of freshmarket strawberries, particularly first quality fruits from October to February in the Irapuato, Guanajuato area. Use of macrotunnels minimizes the risk of frost and results in increased early production, total productivity, and fruit quality, but its high cost limits its use...

Irapuato county, in Guanajuato state in central Mexico, was the main strawberry producer at national level, where viral infections was one of the causes for the decrease in the amount of acres cultivated with this crop. Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV), and Strawberry latent ringspot virus (SLRSV) were previously found...

Ápices de brotes cultivadas en luz y oscuridad de mijo cola de zorra cv Nese 2A fueron crecidos en medio de Murashige y Skoog con cuatro concentraciones del acido 2,4-diclorofenoxiacetico o el acido 2,4,5-triclorofenoxiacetico. En todos los tratamientos, los callos indujeron embriones somáticos y las plantas fueron regeneradas después de las 10 sem...

The recently discovered type VI secretion system (T6SS) is implicated in the pathogenic and/or virulence processes of diverse bacteria. The expression pattern of the T6SS differs among different organisms and also depends on several environmental factors. We initiated a study of the conditions that influence T6SS gene expression in Pseudomonas syri...

Chili pepper (Capsicum annuum) is an economically important crop with no available public genome sequence. We describe a genomic resource to facilitate Capsicum annuum research. A collection of Expressed Sequence Tags (ESTs) derived from five C. annuum organs (root, stem, leaf, flower and fruit) were sequenced using the Sanger method and multiple l...

Table of differential expressed genes. This Excel file contains all differentially expressed genes under effect of bean leaf, pod extract, and apoplastic fluid. The table contains 224 genes that showed ± 1.5 fold change in expression level. Comparative analysis was performed and the genes were grouped in accordance with similar responses. The group...

Table of differential expressed genes with the more stringent cut-off. The table contains 121 genes with ± 2.0 fold change in expression level. These genes were grouped according to the function, and then clustered based on the kind of plant extract using the complete linkage cluster algorithm. The cluster of induced and repressed genes that are di...

Pseudomonas syringae pv. phaseolicola is a Gram-negative plant-pathogenic bacterium that causes "halo blight" disease of beans (Phaseolus vulgaris L.). This disease affects both foliage and pods, and is a major problem in temperate areas of the world. Although several bacterial genes have been determined as participants in pathogenesis, the overall...

In an experimental assay with 12 strawberry genotypes, without protection and under macrotunnel, there was no interaction of the genotype × the production system for none of the parameters evaluated. Macrotunnel increased 12% in average the early production with respect to the former, however both production systems had a similar production for the...

In Irapuato, Guanajuato, México yields of strawberry cultivars are above 40 t.ha-1 but the weight of fruits of 14 g, about 46% of that obtained in California, U.S., where most of the materials were generated. The weight affects the competitiveness in the fresh fruit exportation market. INIFAP and Cinvestav are developing cultivars better adapted to...

México es uno de los 12 países megadiversos y centro de origen y diversidad genética de varias especies, entre ellas algunas con gran importancia para la humanidad, en particular para su alimentación, lo que implica una responsabilidad para la conservación de dichas especies y de sus parientes silvestres. Como parte de las estrategias de conservaci...

On the Central Plateau of Mexico, strawberry yields of 20t/ha are obtained, and have increased only slightly since 1965, due to the poor adaptation of foreign cultivars and their susceptibility to Fusarium oxysporum. Our breeding program, to obtain Mexican strawberry cultivars free from diseases, commenced in 1997. This program aims to develop cult...

Callus and cell suspension can be used for long-term cell cultures maintenance. This chapter describes procedures for the induction of somatic embryos of garlic, keeping a regeneration capacity for more than 5 yr, as well as the maintenance of a tobacco suspension culture (NT-1 cells), for more than 10 yr. Methods for plant regeneration and growth...

Microprojectile bombardment was used to introduce DNA into embryogenic callus of garlic (Allium sativum L.) and produce stably transformed garlic plants. Em-bryogenic calluses, derived from garlic cultivar 'GT96-1', were bombarded with plasmid DNA containing genes coding for hygromycin phosphotransferase and β-glucuronidase. Putatively transformed...

In Pseudomonas syringae pv. phaseolicola the enzyme ornithine carbamoyltransferase (OCTase), encoded by argF, is negatively regulated by argR, similar to what has been reported for Pseudomonas aeruginosa. However, production of the phaseolotoxin-resistant OCTase encoded by argK, synthesis of phaseolotoxin, and infectivity for bean pods occur indepe...

Amaranths are dicotyledonous plants that belong to the Amaranthaceae family which comprises more than 50 genera, distributed in tropical and subtropical zones of the world (Kigel and Rubin 1985). Most of these genera are native to America, and they grow in disturbed areas and produce large seedheads with small seeds. Some of them, like Amaranthus s...

Root apices from in vitro cultured garlic (Allium sativum) cloves of cvs. ABEN and GT96-1 were used as axenic explants for organogenic callus production and plant regeneration experiments. Explants cultured in media based on those of Chu and co-workers (N6) or Murashige and Skoog (MS) could induce organogenic callus after 8 wk culture in darkness....

Mature embryos of Amaranthus hypochondriacus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cultivated on Murashige and Skoog medium supplemented with 10 µM 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-2-methoxybenzoic acid and 10% co...

Green and etiolated shoot apices of foxtail millet (Setaria italica L.) cv. Nese 2A were cultured on Murashige and Skoog medium with four concentrations of 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. In all treatments, embryogenic calli capable of plant regeneration were induced after ten weeks in culture. Calli induced on...

Monitoring for the presence of transgenes by assaying for these using molecular techniques is expensive and may not be a feasible proposal for long-term monitoring. We propose the implementation of a program in Oaxaca to continually monitor the farms, apply questionnaires and keep records of those possible changes in the maize crop that may be attr...