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Publications (20)
Prolonged skeletal unloading through bedrest results in bone loss similar to that observed in elderly osteoporotic patients, but with an accelerated timeframe. This rapid effect on weight-bearing bones is also observed in astronauts who can lose up to 2% of their bone mass per month spent in Space. Despite the important implications for Spaceflight...
Matrix table (pair-wise combination) of common genes.
The 4 genes downregulated by both 0g and PTHrP1-36 treatment are Fos, Zfp36, Pvrlz and JunB.
(TIF)
Complete list of wild-type trabecular osteoblast genes (total 188) whose expression is modified by exposure to 6 days of simulated microgravity (Pthrp
+/+ at 1g compared to Pthrp
+/+ at 0g).
(A) Genes (total 59) upregulated by 0g (6 days). Fold change > 2.0, p < 0.05. (B) Genes (total 129) downregulated by 0g (6 days). Fold change < 0.5, p value< 0...
Complete list of trabecular osteoblast genes whose expression is modified by intermittent PTHrP1-36 treatment (total 163 genes).
(A) Genes (total 102) upregulated by intermittent PTHrP1-36 treatment. Fold change > 1.5, p < 0.05. (B) Genes (total 61) downregulated by treatment. Fold changes < 0.66, p < 0.05.
(PDF)
Summary of experimental conditions aboard satellite Foton M3.
Pre-programmed valves allowed 5 ml (or less) volumes to be fed through the bioreactors. Each PTHrP1-36 treatment was preceded by a 1-min flush, and the 2-h treatment was followed by 2 growth medium rinses and an overnight growth medium feed.
(PDF)
Complete list of trabecular osteoblasts genes (total 763) whose expression is modified by Pthrp gene ablation (Pthrp
+/+ at 1g compared to Pthrp-/- at 1g).
(A) Genes (total 270) upregulated by Pthrp ablation at 1 g. Fold change > 2.0, p < 0.05. (B) Genes (total 493) downregulated by Pthrp ablation at 1g. Fold change < 0.5, p value< 0.05. All probes...
CYP24A1 (hereafter referred to as CYP24) enzymatic activity is pivotal in the inactivation of vitamin D metabolites. Basal renal and extrarenal CYP24 is usually low but is highly induced by its substrate 1,25-dihydroxyvitamin D. Unbalanced high and/or long-lasting CYP24 expression has been proposed to underlie diseases like chronic kidney disease,...
Heme oxygenase-1 (HO-1) encoded by the HMOX1 gene is a 32 kDa stress protein that catabolizes heme to biliverdin, free iron and carbon monoxide. Glial HO-1 is over-expressed in the CNS of subjects with Alzheimer's disease (AD), Parkinson's disease (PD) and multiple sclerosis (MS). The HMOX1 gene is exquisitely sensitive to oxidative stress and is i...
Darinaparsin (Dar) is a more potent cytotoxic arsenical than arsenic trioxide (ATO). We hypothesized that the increased cytotoxicity of Dar may be due to a decreased cytoprotective response. We observe that, unlike ATO, Dar does not induce HO-1, even though is induces expression of other nuclear factor (erythroid-derived 2)-like 2 (NRF2)-dependant...
Overexpression of the pro-survival protein heme oxygenase-1 (HO-1) and loss of the pro-apoptotic tumour suppressor PTEN are common events in prostate cancer (PCA). We assessed the occurrence of both HO-1 expression and PTEN deletion in two cohorts of men with localized and castration-resistant prostate cancer (CRPC). The phenotypic cooperation of t...
Heme oxygenase-1 (HO-1), a member of the heat shock protein family, plays a key role as a sensor and regulator of oxidative stress. Herein, we identify HO-1 as a biomarker and potential therapeutic target for advanced prostate cancer (PCA). Immunohistochemical analysis of prostate tissue using a progression tissue microarray from patients with loca...
Unlabelled:
The mechanisms responsible for oxidative damage, pathological brain iron deposition and mitochondrial insufficiency in Alzheimer disease (AD) remain enigmatic. Heme oxygenase-1 (HO-1) is a 32 kDa stress protein that catabolizes heme to biliverdin, free iron and carbon monoxide. The HO-1 gene is exquisitely sensitive to oxidative stress...
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Prostate cancer is a highly prevalent disease. Despite a significant improvement in the overall survival attributed in part to early detection and introduction of novel therapeutic modalities, many cancer patients at primary diagnosis present advanced disease or experience recurrence of the cancer. The progression of prostate cancer (PCA) to...
Mice with osteoblast-specific deletion of parathyroid hormone-related protein (PTHrP) exhibit impaired recruitment and increased apoptosis of osteogenic cells resulting in decreased bone formation and premature osteoporosis. The PTHrP levels within the bone microenvironment are therefore critical in influencing bone mass acquisition. Whether this i...
Mice heterozygous for targeted disruption of Pthrp exhibit, by 3 months of age, diminished bone volume and skeletal microarchitectural changes indicative of advanced osteoporosis. Impaired bone formation arising from decreased BM precursor cell recruitment and increased apoptotic death of osteoblastic cells was identified as the underlying mechanis...
The use of a novel injectable biocompatible and biodegradable camptothecin-polymer implant for sustained intra-tumoral release of high concentrations of camptothecin is described. The drug delivery vehicle is an in situ thermogelling formulation, which is based on the natural biopolymer chitosan. This formulation, containing homogeneously dispersed...
A method for the determination of the degree of deacetylation (DDA) of chitosan by 1H NMR spectroscopy has been formally validated. Chitosans with DDA ranging from 48 to 100% have been used for the validation. The method is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements. The precisi...
Many lipases and esterases show striking similarities in their enantioselectivities, and empirical rules have been formulated to predict the preferred stereochemistry for various types of reactions. A rule for formation of secondary alcohols predicts which enantiomer reacts faster based on the relative sizes of the substituents at the stereocenter....
The esterase-catalyzed resolution of the cyclic allylic acetates-1-acetyloxy-2-cyclopentene, 1-acetyloxy-2-cyclohexene, and 1-acetyloxy-2-cycloheptene - was not enantioseleclive. We hypothesized that this inefficiency stems from the similarity in size of the substituents at the stereocenter (CH2CH2 vs. CHCH). To increase the enantioselectivity, w...
Compound nameC10H16O4ee = 99.7±0.1% (by 1NMR with (+)-Eu(hfc)3).[α]D= +16.1 (c 0.42, CH3OH) [lit.* +12.4° (c 1.20, CHCl3)]Source of Chirality: Enantioselective enzymic resolution.Absolute Configuration: 1S, 2S * Kasai, M.: Kawai, K.: Imuta, M.; Ziffer, H. J. Org. Chem. 1984, 49, 675-679.
