A. V. Usatov

Southern Federal University | sfedu · Department of Genetics

The study analyzed spatiotemporal trends in the amount, heterogeneity, and extremeness of precipitation in the South of Russia in the period 1960–2022. The highest annual precipitation, at 1634 mm, occurs along the Black Sea coast of the Caucasus. As one moves north and east, precipitation noticeably decreases to a minimum value of 216 mm in the Er...

This work investigated the effect of 10-(6'-plastoquinonyl) decyltrimethylphosphonium (SkQ1) on the regeneration, growth and development of cell and tissue cultures of such plants as sugar cane (Saccharum officinarum L.), spring wheat (Triticum aestivum L.), corn (Zea mays L.), tobacco (Nicotiana tabacum L.), alfalfa of two species (Medicago glutin...

The article presents the results of a study of the influence of plastoquinone derivatives: 10-(6'-plastoquinonyl)decyltriphenylphosphonium (SkQ1) and 10-(6′-methylplastoquinonyl) decyltriphenylphosphonium (SkQ3) on the resistance of grain crops to soil moisture deficiency. The object of the study were seedlings of spring barley (Hordeum vulgare L.)...

Objective: The use of molecular genetic methods in pig breeding can significantly increase the efficiency of breeding and breeding work. We applied the Fst (fixsacion index) method, the main focus of the work was on the search for common options related to the number of born piglets and the weight of born piglets, since today the urgent task is to...

Field peas are one of the most common crops and are grown in various climatic zones. However, the productivity of this crop can be largely limited by climatic factors. This study investigated the influence of climatic factors on pea grain yield in the semi-arid conditions of the Rostov region of Russia in 2008–2020. To quantify climatic factors, ag...

Rice is an important grain crop known for its ability to grow in flooded soil. Sometimes rice plants resist weeds and herbicides’ application ineffectively and unprofitably. The varieties that have flood-resistant genes can help solve this problem, so the study of DNA markers is of great importance. Floodresistant loci contain the Sub1 gene, which...

Fusarium diseases cause serious damage to agricultural crops. In agricultural practice, various chemical fungicides, usually based on triazole, are often used to control fusariosis. This leads to the emergence of resistant forms among pathogenic fungi. An alternative to chemical fungicides treatments may be the use of biopreparations based on natur...

The paper presents the results of candidate genes search for live weight is southern meat sheep breed. The sample of 48 ewes weighed in the age of 24 months and elder was genotyped on Ovine SNP 50 Genotyping BeadChip array. Using a fixation in dices method (Fst), wedetected 55 polymorphisms associated with live weight. 10 SNP swith the highest Fst...

The study of the local sheep breeds’ genetic architecture is an important step on the way to preserve their gene pool. The aim of our work was to identify candidate genes associated with the live weight trait in adult southern meat breed sheep. This breed has high productivity, but is under threat of a critical decrease in genetic diversity due to...

The vast majority of commercial sunflower hybrids worldwide are produced using cytoplasmic male sterility (CMS) of the PET1 type, resulting from the interspecific hybridization of Helianthus petiolaris with Helianthus annuus. In this study, the open reading frame, orfH522, associated with the CMS-PET1 phenotype, was revealed for the first time in t...

Abstract—Based on data from the Remontnoe weather station located in the Eastern natural and agricultural zone of the Rostov region, the seasonal and annual dynamics of the average temperature and precipitation have been evaluated for the period of 1961–2020, as well as the dynamics of hydrothermal conditions expressed by the De Martonne aridity in...

The functioning of the photosynthetic apparatus in barley (Hordeum vulgare L.) after 7-days of exposure to bulk (b-ZnO) and nanosized ZnO (n-ZnO) (300, 2000, and 10,000 mg/l) has been investigated. An impact on the amount of chlorophylls, photosynthetic efficiency, as well as the zinc accumulation in chloroplasts was demonstrated. Violation of the...

Physiological and biochemical characteristics of two varieties of winter wheat seedlings with red (Et W5) and green (Vladi) coleoptiles have been studied. Variety Et W5 with high content of anthocyanins (on average 412 ± 52 μmol/g fr wt), chlorophylls (Chl) and carotenoids and a low level of heme and proline in coleoptiles had high frost resistance...

Trends in mean monthly temperature and precipitation during the growing season and their effects on the maize yield were analyzed at the Zimovnikovsky (Zim) and Rostov (Ros) state variety plots (SVPs), located in different agro-climatic zones of the Rostov region. For these two SVPs, in the period of 1975-2019, the Mann-Kendall test showed a statis...

A comparative analysis of physio-biochemical indices and transcriptional activity of oxidative stress genes in barley (Hordeum vulgare L.) seedlings after 7-days exposure to bulk- and nano-ZnO (300 and 2000 mg/L) was carried out. A dose-dependent reduction in the length and weight of roots and shoots, as well as a significant accumulation of Zn in...

We formed three samples: Landrace (L, n = 86), Large White (LW, n = 120), and Duroc pigs (D, n = 140). Three traits investigated: the mean daily gain (ADG), early maturity (DAY100) and limb condition (LEG; good/bad). The PPARD (rs80828473) and VRTN (rs343248943) genes were selected. The effects of genotypes PPARD on ADG and DAY100 were determined i...

The aim of the work was to conduct GWAS for Total Number Born (TNB) based on the Gradient Boosting Machine (GBM) algorithm, taking into account additive and dominance effects. The study population consisted of 218 Landrace sows. DNA samples were genotyped using GGP HD Porcine. After filtering, 40358 SNPs are left for further analysis. The target va...

Abstract—Trends in the changes in air temperature, precipitation, and hydrothermal conditions, as expressed by the Selyaninov hydrothermal coefficient, were analyzed based on data from six meteorological stations in the Rostov region in the period 1960–2019. A significant trend was determined for all meteorological stations: an increase in the aver...

The significant difference in the mtDNA size and structure with simultaneous slow evolving genes makes the mitochondrial genome paradoxical among all three DNA carriers in the plant cell. Such features make mitochondrial genome investigations of particular interest. The genus Helianthus is a diverse taxonomic group, including at least two economica...

In this study of the Southern Federal District (SFD) in mainland Russia, the spatial nature and temporal trends of the growing season duration, its provision with thermal energy and moisture, and the phenomena of stress temperature for plants were analyzed on the basis of climatic data from 20 meteorological stations for the period 1960–2018. The g...

Background: A significant proportion of perinatal losses in pigs occurs due to congenital malformations. The purpose of this study is the identification of genomic loci associated with fetal malformations in piglets. Methods: The malformations were divided into two groups: associated with limb defects (piglet splay leg) and associated with other...

Breeding practices adopted at different farms are aimed at maximizing the profitability of pig farming. In this work, we have analyzed the genetic diversity of Large White pigs in Russia. We compared genomes of historic and modern Large White Russian breeds using 271 pig samples. We have identified 120 candidate regions associated with the differen...

Data present the chloroplast genome sequences of seven wild perennial Helianthus species obtained by using the Illumina HiSeq and NextSeq platforms. Datasets not included in the primary publication [1] are a source for further evolutionary studies. In particular, the annotated chloroplast genomes and datasets of single nucleotide polymorphisms (SNP...

Sequencing and a comparative analysis of the complete chloroplast genomes of seven perennial Helianthus species were carried out. The chloroplast genomes have a typical quadripartite structure, including large and small single regions and a pair of inverted repeats. Genome sizes were between 151,152 bp and 151,289 bp. The genome of H. grosseserratu...

The effect of the pre-treatment of spring barley (Hordeum vulgare L.) cv ‘Schedry’ grains by agrimitin or SkQ3 (10- (6’-methylplastoquinonyl) decyltriphenylphosphonium) on the plants growth characteristics and the yield during cultivation in 2017 and 2018 under different drought conditions was studied. For the field experiments the pre-treatment...

Reproductive ability of ewes is one of the most important traits in sheep breeding. Hence the mechanisms underlying this complex trait are poorly understood till now. To study the genetic architecture of litter size and search for SNPs associated with Total lambs born (TLB) we conducted genome-wide association study of Volgograd sheep. A total of 4...

The genus Helianthus is a diverse taxonomic group with approximately 50 species. Most sunflower genomic investigations are devoted to economically valuable species, e.g., H. annuus, while other Helianthus species, especially perennial, are predominantly a blind spot. In the current study, we have assembled the complete mitogenomes of two perennial...

Key message Even a point mutation in the psaA gene mediates chlorophyll deficiency. The role of the plastid signal may perform the redox state of the compounds on the acceptor-side of PSI. Abstract Two extranuclear variegated mutants of sunflower, Var1 and Var33, were investigated. The yellow sectors of both mutants were characterized by an extrem...

There has been carried out an estimation of stability of DNA-containing plant samples stored under the conditions that model accelerated DNA degradation. The research material were the lines H. annuus (“VIR 114”, “VIR 116”) and wild-growing forms of sunflower H. decapetalus, H. petiolaris, H. rigidus taken from the collection of the N. I. Vavilov A...

Barley (Hordeum vulgare L.) is one of the most drought-resistant cereals. Water stress causes a negative effect on crop’s seed germination and seedling growth and as a result on the quality and quantity of yield. The rational use of new agrochemicals for seeds pre-treatment is one of the effective techniques to enhance plant tolerance during whole...

Barley (Hordeum vulgare L.) is one of the most drought-resistant cereals. Water stress causes a negative effect on crop’s seed germination and seedling growth and as a result on the quality and quantity of yield. The rational use of new agrochemicals for seeds pre-treatment is one of the effective techniques to enhance plant tolerance during whole...

Background . The problem of nucleus-plastid interactions is obviously of theoretical and practical importance. However, the lack of knowledge in this area of plant research is, for the most part, caused by difficulties in isolating the contribution of the plastome to the formation of complex morphophysiological traits from the total genotypic varia...

A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collect...

Effects of 5-aminolevulinic acid (ALA) at 200 mg/L on photosynthesis, structural organization of photosynthetic apparatus, oxygen uptake rate by the leaf tissue, contents of key respiratory enzymes—cytochrome c-oxidase (COX) and alternative oxidase (AOX)—and COX activity in winter rape plants (Brassica napus L.) were studied. It was found that 4–7-...

This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CM...

Probiotic preparations are used in medical treatment and in agricultural practice. They modulate numerous activities in eukaryotic hosts, such as: inhibition of pathogenic microbiota; stimulation of immunological responses; and production of antioxidants, anti-mutagens, and DNA protectors. Also, probiotic bacteria are used as a preventive measure t...

In the current study, we performed in vivo investigation of probiotic intake influence on nuclear and mitochondrial DNA damage of hens, using quantitative PCR techniques. The probiotic supplementation to the diet of Hisex Brown hens had no significant effect on the rate of telomere shortening. After prolonged probiotic intake (225 and 445 days), th...

Data presents the chloroplast genome sequences of the five sunflower alloplasmic cytoplasmic male sterility (CMS) lines obtained with using the Illumina MiSeq, HiSeq and NextSeq platforms. The sunflower alloplasmic CMS lines has the same nuclear genome from line HA89, but they differ in cytoplasmic genomes, inherited from annual (PET1, PET2 - H. pe...

The study aims at investigating the effect of preparations of two bacilli strains on laying hens and roosters. Preparations were based on the strains Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895. Several groups of roosters and hens received a preparation based on either strain, or a mixture of both, from the first day to the...

Background More than 70 cytoplasmic male sterility (CMS) types have been identified in Helianthus, but only for less than half of them, research of mitochondrial organization has been conducted. Moreover, complete mitochondrion sequences have only been published for two CMS sources – PET1 and PET2. It has been demonstrated that other sunflower CMS...

Copper nanoparticles arise in soil and water from different industries, and well known for their adverse effects on aquatic and terrestrial ecosystems. The soil and hydroponic experiments were conducted to assess the toxicity of excessive amounts of CuO nanoparticles spiked in the soil and in hydroponic conditions on the root and leaf cells of spri...

The role of the GDF9 gene in the process of folliculogenesis, oocyte normal maturation and the development of the embryo promoted an investigation of its polymorphism and testing it as a genetic marker of farm animals' reproductive indices. The purpose of this paper was to study the infl uence of the SNP effect of the GDF9 gene in point G1 (G260A)...

Using Inter Simple Sequence Repeats (ISSR) markers, we studied genetic diversity in five cenopopulations in pedunculate oak (Quercus Robur L.), growing on the territory of Rostov-on-Don region in Russia. Four of five cenopopulations were of natural origin. Total 185 ISSR-markers were identified, of which 184 (99.46%) were polymorphic. Five natural...

Nowadays there are no well-established, standard methods in electron microscopy despite its 50-year history. An excessive variety of research objects prompt researchers to modify and improve methodological approaches to sample preparation. One of the difficult objects to study by electron microscopy is hydrophytic plants, for example, Phragmites au...

Background Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, that is often associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. Investigation of the CMS phenomenon promotes understanding of fundamental issues of nuclear-cytoplasmic inter...

GenBank (MG770607 accession).

GenBank (MG735191 accession).

The study aims at elucidating the effect of bacilli probiotic preparations on the physiology of laying hens and roosters. Probiotic formulations were prepared as soybean products fermented by Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895. In this study, groups of male and female chickens were used. These groups received a prob...

Background . Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, which often is associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. The CMS phenomenon investigations are also promote understanding of a fundamental issue of nuclear-cytopla...

Background . Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, which often is associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. The CMS phenomenon investigations are also promote understanding of a fundamental issue of nuclear-cytopla...

The abundance of potential genotoxins makes it necessary to improve microbial test systems that allow to identify these substances quickly. At the same time, the methodology of metabolic activation of promutagens in vitro is very important. Microsomal preparations of rodents, which are generally used for that, have significant disadvantages associa...

Poultry farming is one of the major directions in agriculture. Agricultural poultry has high growth speed, productivity and is resistant to the industrial environment. Our group carried out a study of probiotic preparations of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 strains. These preparations were made by solid-phase fe...

Background . Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, which often is associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. The CMS phenomenon investigations are also promote understanding of a fundamental issue of nuclear-cytopla...

The complete chloroplast genomes of sunflower fertile line HA89 and isonuclear CMS lines with four different cytoplasmic backgrounds (PET1, PET2, ANN2 and MAX) were sequenced. A total of 451 polymorphic sites, with including 58 SSRs, 317 SNPs and 76 microindels were revealed between the fertile and CMS cytotypes. Among the alloplasmic male-sterile...

Leptin and other hormones secreted by fatty tissue increasingly draw attention as mammalian reproduction markers. In this paper, we have studied the effect of c.3469 T<C SNP of the LEP gene on Large White sows reproductive traits according to all litters and differentially according to each litters (1st, 2nd and 3rd litter). Research was conducted...

Effects of exogenous 5-aminolevulinic acid (ALA) on development and certain biochemical parameters of winter rape plants (Brassica napus L.) were studied. Plant growing on 50–200 mg/L ALA solutions for 7 days resulted in an accumulation of anthocyanins in cotyledonous leaves and changed in leaf color from green to violet. An application of 200 mg/L...

The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers – HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant character of the HRG01 marker and HRG01 amplicon...

Rice is a unique crop which is generally grown in submerged soil. However, not all rice varieties according to its genotype can tolerate to complete flooding. The selection using DNA markers is now frequently used in real breeding programs, including for the development of submergencetolerant rice. This review summarizes the researches devoted the...

The aim of our work is to identify the single and combined effect of MC4R and GH polymorphism genes on growth and reproduction traits of pigs. The study was carried out on 204 crossbred F1 females (?Landrace ? ?Large White) of the Breeding Farm in Russia. The analyzed productive traits were: The number of Days to 100-kg (Days to 100 kg), Length of...

The most prospective and actual trend in farm animal breeding is studying gene polymorphisms affecting the productive traits. The objective of this investigation was to study the effect of polymorphisms in intron 1 of the swine POU1F1 gene on number of days to 100 kg (days to 100 kg), length of body (LB), backfat thickness (BF), and number of pigle...

Birds are unique models for molecular studies on aging processes and cellular senescence because of their slow rates of aging and comparatively long life spans among homoeothermic animals. The study aimed to examine the relative telomere length and amount of nuclear, mitochondrial DNA lesions in hens of different ages and to find out the correlatio...

The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorop...

Chlorophyll deficient mutants are appropriate model objects for a large number of investigations in the field of photosynthesis. In this study, we identified a state of Photosystem I (PSI) and Photosystem II (PSII) photochemistry and light responses of the sunflower yellow-green mutant line-en:chlorina-7, wherein mutations in chloroplast genes have...

The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A...

Marker-assisted selection (MAS) was adopted for introgression of broad-spectrum blast resistance genes Pi1, Pi2 and Pi33 into elite Russian rice varieties. It was shown that microsatellite markers RM 224, RM 527 and RM 310 may be effectively used to transfer Pi1, Pi2 and Pi33 genes into selected Russian genotypes of rice. Based on the highly produc...

Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf...

The entire chloroplast and mitochondrial genomes of domesticated and wild type sunflower were sequenced. The comparative analysis of chloroplast genomes revealed 43 variant sites, including 21 polymorphic SSR loci and 22 SNPs. About 14 variant sites were found by collation of mitochondrial DNA (mtDNA), among them 4 SSRs, 8 SNPs and 2 deletions. Abo...

Rice (Oryza sativa L.) plants were subjected to NaCl stress within 35 days. The shoot dry weight, root dry weight, total survival percentage and contents of inorganic ions in dry samples of stressed plants were measured to identify the physiological adaptive mechanisms by which rice plants at vegetative stage tolerate salt stresses. Laboratory test...

The present work is devoted to investigation of the possibility to apply methods of Marker-Assisted Selection (MAS) to introgression the SalTol QTL into genotypes of elite Russian varieties of rice. It was shown that microsatellite markers Rm493, may be effectively used to control transfer the SalTol QTL genes into Russian populations of rice. Base...

The polymorphism of microsatellite loci of chloroplast genome in six Helianthus species and 46 lines of cultivated sunflower H. annuus (17 CMS lines and 29 Rf-lines) were studied. The differences between species are confined to four SSR loci. Within cultivated forms of the sunflower H. annuus, the polymorphism is absent. A comparative analysis was...

The purpose of this study was to determine the frequencies of alleles and genotypes of the Leukemia Inhibitory Factor (LIF) gene (ID: 399503) in Landrace, Large White and Duroc pigs in Russia and to evaluate the impact on productive traits of pigs. Polymorphism was genotyped using PCR-RFLP method. In the course of studies we observed a different di...

The aim of this study was to determine associations between genotypes for the prolactin receptor (PRLR) gene and swine reproductive, growth and meat traits. 210 sows of three genetic groups: Large White (LW), Danish Landrace (LD) and Landrace x Yorkshire x Duroc (L x x Y x D) were included. The studied reproductive traits included the total number...

Identifying the best inbred combinations for the development of commercial hybrid of sunflower remains the main challenge to sunflower breeders. In the present research the level of heterosis of F1 hybrids, genetic diversity of parental lines based on SSR markers, as well as its connection with specific combining ability of sunflower were studied....

The Melanocortin-4 Receptor (MC4R), a G-protein coupled receptor, is implicated in mediating the effect of leptin on food intake and energy balance. A previous candidate gene study reported an association between an MC4R polymorphism (Asp298Asn) and growth and meat productive traits of pigs. The aim of this work was to determine frequencies of the...

One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of th...

One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resis tance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of t...

The 7- to 8-day-old barley (Hordeum vulgare L.) seedlings grown in KNO3 solutions (1-40 mM) were characterized by the substrate activation of nitrate reductase (NR) in the apical leaf segments (1–2 cm in length), as well as by stimulated growth, broadened leaf blades, and by vigorously developed system of shortened roots. When the seedlings were gr...

The effect of bio-stimulants such as gamavit, citrated blood and polivitam on biochemical and hematological parameters in porcine blood plasma has been investigated in this work. Concentrations of biochemical parameters (total protein content, albumin, alpha-globulins, beta-globulins, gamma-globulins) and hematological parameters (the number of leu...

The uniqueness and high commercial value of the relict group of sturgeon has stimulated studies of their genetic polymorphism. In this review, we discuss the main molecular-genetic markers used for estimating genetic variation and their drawbacks and advantages, and we give examples of their application, mostly for Eurasian Acipenseriformes. Proble...

Growing barley (Hordeum vulgare L.) plants for 7 days on NaCl solutions (20–200 mM) decreased chlorophyll (Chl) a and b content with respect to that in untreated control plants. The content of free proline and the plant ability to synthesize 5-aminolevulinic acid (ALA) started to increase in parallel at salt concentrations of 20–50 mM. The maximum...

Uniqueness and high commercial value of relic group of sturgeon fishes stimulated researches of their genetic polymorphism. In the review the basic molekular-genetic markers used for an estimation of genetic variability are considered; their merits and demerits are discussed, examples of their application, basically, on Acipenseriformes Eurasia are...

The influence of high temperatures on sunflower chlorophyll mutants was studied. The tolerance criteria were the level of chromosome aberrations and the mitotic index in the root apical meristem of seedlings, the level of nucleus-free cells in the epidermis of cotyledonous leaves, and the intensity of chlorophyll accumulation after the action of he...

Three populations (Azov, Caspian, and Black Sea) of Russian sturgeon Acipenser queldenstaedtii were tested for polymorphism at nuclear (RAPD and microsatellites) and mitochondrial (PCR identification of two mitotypes) markers. In addition, morphometric analysis of he representatives of Azov population was carried out. According to the morphological...

Ultraviolet radiation with a wavelength of 300–400 nm is characteristic of sunlight at the earth surface and causes DNA damage mediated by energy transfer to O2 with the transformation of the latter in the singlet state. In connection with this, scavengers of reactive oxygen species (ROSs) are potential protectors against the genotoxic effect of th...