Central Salt and Marine Chemicals Research Institute
Question
Asked 14th Mar, 2013
Will phycoerythrin pigment appear as purple ?
Recently I extracted pigment from filamentous cyanobacteria, which is purple color during the ammonium sulphate saturation from(35-65%). Normally phycoerythin appears as deep red to pink.
Absorption spectra also showed the peak between 540 -560 nm.
I also attached my phycoerythrinfor verification.
Most recent answer
Take UV spectra of the sample....you will find additional peaks at 620nm, if so then phycocyanin is present and that is giving violet colouration
All Answers (8)
Ministry of Environment, Forests and Climate Change, Govt. of India
It appears to be little bluish. Please check the presence of phycocyanin.
Centrum Algatech
Well it can appear purple as there are different shades of Phycoerythrin depending upon their shoulder peaks, check their shoulder peaks. May be you can conclude
1 Recommendation
Indian Institute of Technology Guwahati
It is phycoerythrin.....no worries. Sometimes a very concentrated erythrin may look like this. Even if the majority of the pigment is erythrin, cyanin will also be present.
Indian Institute of Science Education and Research Kolkata
I think it is a mixture of Phycocyanin and phycoerythrin thats why look like this.
1 Recommendation
curador sa
Working with phycocyanin extracts, it seems that the volume of the flask and the exposition to light could dramatically influence the color. In this case, with phycocyanin form laggon blue to purple/red. Magy of light properties
Algallio Biotech Private Limited, Vadodara
Algae have others pigments like Phycocyanin, allophycocyanin also,they are in blue color.
The resulted mixture will appear like purple, pinkish blue, Its shade will be depends on concentration of different phycocbiliprotein
Similar questions and discussions
Related Publications
The functions of phycobiliproteins and phycobilisomes as photosynthetic antenna pigments in cells of cyanobacteria and a range of algae were considered. Achievements in the area of biological and natural sciences connected with study of phycobiliproteins are described. Sources and different possibilities of the practical application of these pigmen...
Thesis (Ph. D.)--University of California, Berkeley, 1988. Includes bibliographical references (leaves 275-286). Photocopy.
Phycobiliproteins are mainly found in blue-green algae, red algae and some species of cryptophytic algae. They function as major light-harvesting complexes to harvest and transfer sunlight energy. Meanwhile, phycobiliproteins are also fluorescent proteins with high fluorescence quantum yield, and in consequence be widely concerned in both theoretic...