University of Bonn
Question
Asked 26th Aug, 2013
What is an economical alternative to dialysis tubing for removing unreacted label dye from polymer particles?
After coupling reaction of Texas Red dye with nanogel particles I need to remove any unreacted/unwanted dye from the polymer dispersion. My sample volume is around 40mL which means I have to use large quantities of buffers for dialysis, which is both a bit expensive and time-consuming (I should change the buffer everyday for at least 5 days). I can not use water because of Texas Red hydrolysis!
Most recent answer
If you know the molecular weight of your polymer, you could use filtration units with a specific size exclusion membrane (e.g. Millipore offers such devices). They are used in fixed angle or in swinging bucket rotors, have 50 ml Falcon tube size, they take 15 ml at once and they can be used many times. Thus the initially high price is not very high. Once used, you have to keep the filtration membrane wet and to add a preservative like EDTA or NaN3 (keep it at 4°C). Filtration times are ca. 15 min at 3000 g.
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Popular answers (1)
SyMO-Chem
Centrifugal ultrafiltration may be a good solution for your problem. It may require a bit of optimization, but it is certainly faster than your dialysis protocol.
An appropriate Sephadex column is perhaps also an option, depending on how big your nanogel particles actually are.
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All Answers (10)
SyMO-Chem
Centrifugal ultrafiltration may be a good solution for your problem. It may require a bit of optimization, but it is certainly faster than your dialysis protocol.
An appropriate Sephadex column is perhaps also an option, depending on how big your nanogel particles actually are.
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National Sun Yat-sen University
One way you may try is to use solvelt-nonsolvent for the separation. Typically, polymer has narrow range of solubility to be solved, but small moleucle has wider range.Choose one solvent that your polymer is not dissolved, but the dye molecule dissolved. After that, centrifugal ultrafiltration suggested by Serge should be useful.
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leon-nanodrugs
The size of the nanogels is 550nm and prepared in sodium phosphate buffer.
Thank you for your answers, I think Ultrafiltration might just be my best try.
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National Institute of Technology Rourkela
Blerina,
sonication by ultra centrifuge may another option for you.
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Soxhlet extraction using a selective solvent may help,but I do not know the stability/ and other paramter .....................!!!!!!
Ghent University
I think the centrifugation is the best? but need to be careful with speed for avoiding the aggregation or destruction. But all depence in stability of your particles.
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Zhejiang University
Soxhlet extraction using a proper solvent is the best way. It costs some time, but it is unnecessary to keep an eye on it. You can do something else during the extraction.
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University of Bonn
If you know the molecular weight of your polymer, you could use filtration units with a specific size exclusion membrane (e.g. Millipore offers such devices). They are used in fixed angle or in swinging bucket rotors, have 50 ml Falcon tube size, they take 15 ml at once and they can be used many times. Thus the initially high price is not very high. Once used, you have to keep the filtration membrane wet and to add a preservative like EDTA or NaN3 (keep it at 4°C). Filtration times are ca. 15 min at 3000 g.
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