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Question
Asked 16th Jul, 2012

What exactly is the function of DMSO in PCR?

It decreases the Tm but does it have any other function during PCR. I tried my PCR with temperature gradient 1.5 degree celcius less as calculated after adding DMSO, but my PCR did not work but when DMSO was added it worked.
Murthy Chavali
University of Wuerzburg
try using glycerol