What cell marker would stain dermal fibroblasts but not keratinocytes?

I am expressing two bacterial proteins in E.coli

one is truncating and appearing 7 KDa below the actual size on sds.

another protein is forming oligomer or dimer and giving extra band at higher molecular weight along with normal protein, i am visualizing two bands of proteins expressing at different size on sds. suggest me the future trouble shooting..

thanks in advance.

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment.

Thank you in advance

I am finding it difficult to dissolve DAPI in water as many protocol mention distilled water or PBS

How to dissolve DAPI stain?

Our lab bought 20 microgram of Lyophilized(freeze-dried) IFN-g for research purpose. In paper reviewing I found everyone using 10,100,1000 IU/ml etc for MTT assay or other purpose. But I can't understand how they can convert the concentration from microgram/ml to IU/ml? For information, the specific activity is <0.05ng/ml corresponding to a specific activity of 2.0*10e7 IU/mg. Thanks in advance.

I plan to stain fibroblast and keratinocytes in frozen sections from skin.  Can anyone suggest a good marker (target and a good antibody)? What is important – I’m looking for two antibodies for keratinocytes to differ highly differentiated keratinocytes from undifferentiated ones. Thank you!

Thanks in advance.

We're setting up a new skin tissue engineering lab from scratch and I wondered if anyone had any thoughts on the best commercial suppliers of dermal fibroblasts and keratinocytes, and their associated media? In my previous lab we used freshly isolated cells and 'home-made' Green's media for the KCs but that won't be possible in my new lab. Or alternatively, are there any that we should avoid? Thanks!

Human fibroblasts seeded into a type I collagen gel with subsequent keratinocyte layer maintained at the air-liquid interface.

Hello,

I want to stain MCF7 cells treated and untreated with beta- Gal.

My treatment lasts for 12 days, then I harvest the cells.

Does someone know how I could treat MCF7 cells to get a positive Control within max 10 days ? or even less ?

or any other way to treat a different cell line and get quickly Sen induction ?

I only know that irradiation of primary fibroblasts can induce Senescence and therefore beta-Gal positivity, but upon irradiation I would need to culture them 40 days and I don't have that time at the moment.

Thanks a lot in advance