What are conserved archaea proteins I can use in my Western blots?

Is there any specific mutated strain regarding this isolation?

We were doing the monoclonal antibody generation. After the western blotting incubation with IRDye and infrared fluorescence detection. Two bands were found on the sample column of the membrane. The light band is much thicker than the heavy band. What is the reason behind? Because I thought the heavy chain should have a thicker band as its more bulky?

I'll compare human and Archaea mucus

A small peptide has been loaded on Archaeosomes.

To determine the loading quality and quantity efficiency we tried tricine- SDS PAGE. Now the problem is how we can eliminate the free small peptides, those not really loaded on archaeosomes.

which region is selected for amplification of 16S rna of Archea?

Good afternoon,

I am searching compound able to arrest hyperthermophilic archaea into G1 phase (if possible) to synchronize them and study S phase. Do you have any idea about this kind of compound ?

Thanks,

Rémi

We have produced a recombinant of DNA polymerase from a hyperthermophilic archaeon. We want to see the activity difference of native enzyme with a few mutants and effect of some inhibitors. A kit with non-radioactive and simplest method is required. If there is some option to proceed a few reactions (10-20 reactions), it will be really helpful for us.