SOLVENT SYSTEM FOR separation of lipids by Thin layer chromatography ?

2 Recommendations

Naser Jawad Kadhum

University Of Kufa

According to my experience in this field

In each client separated by TLC, several types of mobile phase must be used

Then depend on the mobile phase that gave the highest number of compounds

The best separation between vehicles without interference

You can also use two-dimensional

First, use a single number moving phase

Then use another phase after turning the plate on its side

In this way. Use one spot of the extract on one side of TLC

After the completion of the migration, flip on the side where you are traveling and place in the second mobile phase.

This method ensures a clear contrast of compounds without interference

6 Recommendations

Md Osim Aquatar

Academy of Scientific and Innovative Research

Frank T. Edelmann

Otto-von-Guericke-Universität Magdeburg

Abdelkader BOUAZIZ

SOUGUEUR Division - Chemistry (POLYMERS), Université Ibn Khaldoun Tiaret

Aref Wazwaz

Dhofar University

Jürgen Wintner

Solvias AG

Naser Jawad Kadhum

University Of Kufa

According to my experience in this field

In each client separated by TLC, several types of mobile phase must be used

Then depend on the mobile phase that gave the highest number of compounds

The best separation between vehicles without interference

You can also use two-dimensional

First, use a single number moving phase

Then use another phase after turning the plate on its side

In this way. Use one spot of the extract on one side of TLC

After the completion of the migration, flip on the side where you are traveling and place in the second mobile phase.

This method ensures a clear contrast of compounds without interference

6 Recommendations

Noemi Tejera

EMBL-EBI

Hi Nabin

When analysing lipid clases, I use hexane/diethyl ether/acetic acid (80:20:2, by vol), as neutral solvent, to separate free fatty acids, triacylglycerols, cholesterol and esterol esters. You will leave the polar fraction (all the phospholipids) on the bottom of the plate.

If using some phospholipids rich source, you could also go for a double development, using methyl acetate/isopropanol/ chloroform/methanol/0.25% (wt/vol) KCl (25:25:25:10:9, by vol) first, to separate the phospholipids. Then you can leave the plate dry and run again, using the neutral mix this time. In that way you could see all lipid classes.

Because you are looking into use oils or butter, I would go for the development using the neutral mix.

Petroleum ether is less pure than hexane, contains hexane but also other hydrocarbon compounds, but it is also cheaper. So I would use the first mix that you suggest if you can't use hexane.

Any thoughts about the dye you are going to use? I have worked with 2′,7′-dichlorofluorescein in methanol, but you would need an UV lamp to see the bands. I also have used 1% iodine in chloroform. That would be more straight forward, because you could see the bands right away.

I hope it helps!

Noemi

Ahmed M. Sayed

Nahda University in Benisuef; Almaaqal University in Basrah - Iraq

For such type of lipids i use 100% chloroform or 100% dichloromethane. Then visualize the separated spots by para anisaldehyde reagent.

Note: Such type of oils mainly contain triacylglycerols which are un separable compounds, so some oils will appear as a single spot.

Ali Hassan Abood

University Of Kufa

good answer Naser Jawad Kadhum Naser Jawad Kadhum Harith Rajab Almosawy Dhifaf Zeki

Why is the power bank used for western blots not reaching the correct voltage?

2 Recommendations

We have a fisherbrand powerbank (cat. FB300Q) that we use for our western blots. During the transfer, we noticed that it would not reach 90 volts and only get up to 77 volts. We thought it might have been the electrodes, so we switched out the cassette and lid, but unfortunately, that didn't resolve the issue. We also thought it might not be cold enough, so we put a fresh ice pack in the chamber, but that didn't work as well. Also, in the past, we used to put our transfers in the cold room (large walk-in 4C) and sometimes the power banks would switch from volts to amps.

Asked 8th Aug, 2023

Jasmine De Mange

Hello,

Has anyone else ever experienced this during a transfer? Could this problem be mechanical, or something else?

Thanks for all your help!

How can I access GraphPad Prism for free?

Hello ResearchGate community

Asked 14th Jul, 2023

Francesco Errichiello

I need your help. I previously used the trial version of GraphPad Prism for data analysis, but it has expired. Now, I have more data to analyze and would appreciate any guidance on obtaining free access to GraphPad Prism. If you have any suggestions, please share your insights.

Would you participate at the INTERNATIONAL CANCER IMMUNOTHERAPY CONFERENCE (CICON23) in Milan, September 20th to 23rd, 2023?

Dear Colleagues and friends,

Asked 10th May, 2023

Pier Francesco Ferrucci

as Co-Chairman, I would like to invite you to join us and present your work at the VII INTERNATIONAL CANCER IMMUNOTHERAPY CONFERENCE (CICON23) – TRANSLATING SCIENCE INTO SURVIVAL, which will be held in Milan from September 20th to 23rd, 2023.

Launched in 2015, under the patronage of the Cancer Research Institute (CRI), the American Association for Cancer Research (AACR), and the European Network for Cancer Immunotherapy (ENCI), the Conference continues to provide groundbreaking discoveries, being the ideal place to promote the fast- moving field of immunology and immunotherapy.

We have assembled a line-up of outstanding speakers, including the Nobel Laureate Jim Allison together with Arlene Sharpe, Tony Ribas, Lisa Coussens, Nir Hacohen, Padmanee Sharma, Ozlem Tureci, Laurance Zitvogel, and Shannon Turley just to name a few. At the same time, we dedicated slots for talks selected from the best abstracts, opportunities for short oral posters presentations, and ample time to discuss the data.

Our goal is to provide an exciting and unique opportunity for all attendees, students, postdocs and PIs, to meet top scientists and talk with colleagues in an informal environment.

Some additional information about the Scientific and Social Program could be found on the site, http://www.cancerimmunotherapyconference.org/program-of-events

You can submit your abstracts here: http://www.cancerimmunotherapyconference.org/abstracts, till the 15th of June, 2023, so, please, use your chance and submit your abstract now!

For registration please go to http://www.cancerimmunotherapyconference.org/registration.

We particularly encourage students and postdocs to attend the conference and, in order to facilitate their presence, we offer special registration rates and travel bursaries, which can be applied for during the abstract submission., so, get your ticket now for the early bird price!

If you have any questions or need further information, please do not hesitate to contact me.

I look forward welcome you at the COCON23!

Best regards

Dr. Pier Francesco Ferrucci

Chair CICON23,

President of the Italian Network Tumor Bio-immunotherapy (NIBIT),

Scientific Director Grazia Focacci Foundation,

SEMM Faculty,

Director of Tumor Biotherapy Unit,

IEO - Istituto Europeo di Oncologia - IRCCS

Via Ripamonti 435 - 20141 Milano, Italia

T: +39 02 94371094 E: pier.ferrucci@ieo.it W: http://www.ieo.it/it/CHI-SIAMO/Come-siamo-organizzati/Le-divisioni/Unita-di-Bioterapia-dei-Tumori-BTTUN/

If this message does not display properly, please click here to view it online in your browser

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ABSTRACT SUBMISSION DEADLINE JUNE 15, 2023

Someone knows how to crack graphpad Prism 9?

i need urgently. Thanks!!

Asked 10th Mar, 2023

Manuel Cabadas

How to make 2% of nitric acid solution in 100ml of water?

How can I make a 2% of nitric acid solution in 100ml of water? if you don't mind please show with calculation so I to understand better.

4 answers

Asked 20th Oct, 2022

Edzani Mukonazwothe

Hello please assist,

Thanks.

Protein-protein molecular dynamic simulation in gromacs

Discussion

9 replies

Asked 5th Oct, 2022

Sahadot Hossen

I am seeking a pdf or doc file . #Molecular_Dynamic_Simulation #Gromacs #Protein-Protein MDS Hey, I am working on a project where MD simulation is needed for protein-protein interaction in a dynamic nature. I have previous experience of doing protein-ligand MD simulation from Lemkul's tutorial. Now I am facing a few problems, including 1. In the protein-ligand case, first build the protein topology and then the ligand's one. In the ligand topology generation section—another server used to obtain itp files and introduce them to the protein topology file— In protein-protein MDS, what should we do? Where we get the topology of another protein.

How to install Autodock Vina via pip or conda in Windows?

I trying to install vina using pip and get this error(see file). I think the problem is that boost is not added to path. I have tried various tips posted on stackoverflow threads, read the boost docs for working with python and even watched the videos about how to install boost, but have not been able to solve this problem.

5 answers

Asked 3rd May, 2022

Artem Petrov

Apart from using pip I tried to install via conda following this manual:

https://autodock-vina.readthedocs.io/en/latest/installation.html#building-from-source

Error not fixed. How i can add boost to path? After installing boost via bootstrap.bat and then .\b2 i get 2 links, but if i add it to PATH variable it doesn't solve this problem. I want to try use Vina scripts in python file

Has anyone been able to install Autodock Vina via pip or conda?

Brief output:

...

ValueError: Boost library location was not found!

Directories searched: conda env, /usr/local/include and /usr/include.

I´m looking for the best configuration to build a workstation to run molecular dynamics simulations of proteins in microseconds. It´s even better with the GPU is compatible with CUDA 11 (NVIDIA).

8 answers

Asked 22nd Jul, 2021

Samilla B. Rezende

How much difference of KD is apparent?