Recommendations for adiponectin and leptin kits for mouse plasma?

Good afternoon,

Currently my team is performing animal studies with majority of the time using rats of different strains.

Our latest experiment was a weight loss study with diet-induced obesity SD rats, where now, as part of the analysis, we need to measure the following:

- HbA1c

- Insulin

- Adiponectin

- Leptin

- Ghrelin

- GLP-1

- Glucacon

Were have those samples as serum (except the HbA1c which is whole blood) and would be open to options when it comes to the analysis: different options for analysis and where to perform.

I've been having trouble in finding a company that can provide a service for the analysis of those above. I am aware one could purchase ELISA kits for those, but would prefer to outsource to a company, since the results of ELISA can vary greatly depending on the ability of the person performing it.

Thank you,

Larissa

We will carry out dose-response tests with applications of different concentrations of the proteins mentioned (leptin, adiponectin, melatonin and BDNF) in the whole blood of male and female individuals. However, we did not find in the literature articles that used these stimuli in whole blood (starting point).

The cost estimates of a planned study are mainly based on serum blood. However, to reduce particiants burden, we would like to choose dried blood spot. Therefore, it would be very helpful if anyone could provide some approximate cost estimates for assaying the following biomarkers via DBS:

- zinc ($5 ??)

- transferrin

- Vitamin A & D ($3 each ???)

- IL-6, TNF-alpha, CRP, leptin ($ 9.5 ???), adiponectin

Thank you very much.

Hello everyone, I need to evaluate leptin levels in the serum of rats in vivo treated with silica nanoparticles. I have difficulties in finding robust commercial ELISA assays to evaluate this and other adipokines (for instance, I tried to measure adiponectin by commercial kits providing pre-coated plates, but it was impossible to have results since the standard curve did not work). Do you have any suggestion based on your experimental experience?

Thanks in advance.

I am working on a study to examine NAFLD/NASH disease progression in patients suffering from metabolic syndrome. I am looking to see if anyone has suggestions for potential biomarkers to analyze aside from the typical markers such as adiponectin, leptin, etc. Thanks. 

These tests are required for research purpose. Cost for these tests and contact numbers are required

Hello,

I am working on a project about the involvement of oxidative stress in obesity. Does anyone have experience with adiponectin and/or leptin measurement using microplate readers (FluoStar Omega in particular)?