Quatification of exopolysaccharides (EPS) in filamentous cyanobacteria

Want to know how efficient well diffusion is over disc diffusion

How band gap varies in this structure?

I found a bloom of cyanobacteria with filaments that look like Sphaerospermopsis aphanizomenoides if you see the dispositions Heterocyte_AKinete_Heterocyte and others features but i found some filaments with an elongated apical cell (fusiform) (i have tried to find Heteocytes or AKinetes but i didn't find).

Some suggestions?? Perhaps Aphanizomenon favaloroi?

Phytoplankton from a Reservoir fixed with lugol

Thanks in advance

Aphanizomenon flos aquae (AFA) is a filamentous prokaryotic bacteria that is also known as a Cyanobacteria or Blue-Green Algae. AFA may be harvested from fresh water lakes, ponds or rivers, and dried to produce a powder that is consumed by humans.

I am doing my PhD in cyanobacteria causing off-flavor in the water (Mycrocystis, Anabaena, etc), I found the isolate in the pond (Mycrocystis), but when I cultured and purified the Mycrocystis can't grow, but the other species grow well, I tried in mediums with different N:P ratio, 1:1, 1:5, 1:10, 1:15, 1:20, all show the same result. Anyone can help me, or give me suggestions. Thanks

I want to culture few cyanobacteria like Anabaena, Nostoc calothrix etc, in large scale for getting biomass.

Can anyone tell me the suitable media for this culture? BG11 media not giving so much biomass. I am culturing in Glass tank of 36"*36"*26"

 please give reference also.

I am interested in using flow cytometry to measure blue green algae (cyanobacteria) populations in freshwater samples. However, most of the species we find are colonial (Woronichinia sp) or filamentous (Anabaena sp. and Oscillatoria sp.). How can I best separate these colonies and filaments into their single cells, without damaging or lysing the cells? The purpose of this is to allow the single cells to pass through the flow cytometer one cell at a time. Does anyone know of any routine procedures using vortexing or sonication?

Many thanks.

I am trying to extract cyanobacteria exopolysaccharides from soil samples, using an EDTA extraction followed by a carbohydrate analysis with the phenol-sulfuric acid method (Dubois et al., 1956). My samples are precipitating and turning cloudy after the addition of the phenol and sulfuric acid. Any insight on why this is happening or how to fix it?

The spectrophotometer peaks look OK, just the baseline is raised depending on how cloudy the sample is. Is it valid to subtract the baseline from the reading? Or even better, how do I get rid of the cloudiness? Is it a pH issue? Thank you!

I am growing Anabaena sp. PCC7120. They seem to grow well on agar plates containing BG11 and BG11o. However, they die after 3 days when I transfer my cells to liquid BG11 or liquid BG11o. I am growing my cells at 28C under 50 umol/m2.s PPFR and gentle agitation of 80 rpm.