Please identify this endophytic fungi ?

I am working diversity of endophytic fungi and I have followed the Hawkworth classification of fungi for my research work and already submitted my research data for publication but recently I have noticed updated classification system (Outline of fungi) given by Wijayawardane et al., 2022 so what should I do now? Is it ok to fallow the Hawksworth classification or should I change ? need clarity...

Hello,

I recently received metagenomic 16S rRNA gene sequence data from a company, which includes both raw reads, and clean data with barcodes removed. My goal is to analyze these sequences and obtain information on the taxonomic diversity and abundance of the species present in the sample.

Since I use a Windows system and cannot utilize Mac or Linux, I would greatly appreciate guidance on how to proceed with this analysis. Are there any web server-based applications available that can assist with this task?

Furthermore, if there are any researchers or experts interested in this project, I would be grateful to explore potential collaborations. Please feel free to reach out to me if you are interested or have any recommendations.

Thank you in advance for your assistance.

Best regards

which method should be more succeful and realabel in idetifing Microbiota

Metataxonomics or the shutgun ?

Hi, Everyone

I want to check the secondary metabolites of endophytes whether endophytes have similar phytochemicals or not. I have lots of endophytes isolated from medicinal plants. Kindly suggest to me what should I do?

Endophytic fungi are ubiquitous to plants and are mainly members of Ascomycota or their mitosporic fungi, and some taxa of Basidiomycota, Zygomycota, and Oomycota. These endophytic fungi reside within living tissues of the host and establish symbiotic relationships . The colonization of plants by these fungi varies considerably; and some medicinal plants are reported to harbor more endophytes . The endophytes associated with medicinal plants are known to acquire the potential to produce the active principles for which the host is known .They are a rich source of functional secondary metabolites that include flavonoids, terpenoids, steroids, phenol, phenyl propanoids, quinines, indole derivatives, amines, alkaloids, amides, pyrrolizidines, sesquiterpenes, diterpenes, lignans, isocoumarinated chloride derivatives, metabolites, phenolic derivatives , aliphatic compounds.

I have isolated the endophytic fungi from grasses by surface sterilization method but how to proof that the sterilized tissue doesn't contain any epiphytes, is their any techniques or test to determine that the surface sterilized tissues are epiphytes free.

Can anybody explain me the interaction of endophytic fungi and the host in the production of secondary metabolites. I just need to know that if the endophytic fungi and the host plant producing same metabolites means what type of interaction or mechanism is going on between them and if not means what...!

I am working on grass fungal endophytes bioprospecting, I have isolated the sec. metabolites by using Ethyl acetate and Methanol for culture filtrate and mycelial mat but I am having a small doubt that why can't we isolate fungal metabolites by sequential isolation by using various solvents. If yes, then which are the solvents used for it.

I'm working on transmission of endophytic fungi in grasses. I need to observe the fungal hyphae growth in grass tissues. Hence, I need an best protocol for the study.