I am trying to incorporate some other Acrylates with Dopamine molecule. Can anybody recommend me these type of water soluble polymers ??

I want to assess the cell migration rate through scratch wound assay for the hydrogel. I have read the literature and in some research articles it is mentioned that DMEM media was not supplemented with FBS (10%) as it supports the growth of the cells so it will effect the results. But in other studies only term culture media has been used. Kindly guide me whether FBS should be added or not into the DMEM.

Thanks in advance for your guidance and time.

Hi, I have some troubles since few weeks cultivating my cells on matrigel-coated plates (Neural progenitor cells on the picture). At some point, I observe some cell debris-like things that seems to grow and cover the whole well after few days, but I’m not sure about the type of contamination. I thought at first that the matrigel was « degraded », but I’m not sure about that. It’s not moving but I don’t recognize this shape. (I also attached a BF picture for more contrast). Does anyone have an idea? Thank you for your help!

I want to synthesize Schiff base from Benzil and 2-aminopyridine by 1:1 ratio so that one carbonyl group (c=o) of benzil remains unreacted. Which process can I follow?

Based on my literature survey, I have seen that 24 hours for cell adhesion is recommended. The articles or standard video tutorials do not mention of attainment of morphology as an essential pre-requisite of MTT assay.

I am working with SHSY5Y which takes ~65 hrs to divide and more than 24hrs to attain morphology when seeding 15k in 96 well plate. I have been treating them with my stressor after 24hrs, and have been consistently getting no variation across Normal control and Treatment group.

The lack of variation can owe to the does of stressor, but my question is can the morphology matter significantly? I am treating them without attainment of morphology.

I would like to buildup a small active research group including a comprhensive subspecialities in Clinical Biochemistry, Molecular Biology, Internal medicine, statisticians to be shared in writing research articles, review, chapters and books. Who see him a suitable he can comment here with his email or whatsapp no to communicate later.

I tried to revive a2780 cells cryopreserved in -80 degrees. Its been more than 12 hours and its still not attaching. What could possibly be happening? What should I do?

I can’t find the suitable conditions when I react hydrazide with aldehydes. I tried to stir in RT and at reflux for days. Also, I used different solvents, like; abs ethanol, glacial acetic acid, dry DMF, DCM. In addition to adding 1ml of glacial acetic acid or few drops of triethylamine as a catalyst. But, there’s no reaction and the start material (hydrazide) is the same "no consumption"

how to convert .jdf NMR file to Topspin compatible format?

I have synthesized a Schiff base compound but everytime I do NMR I found an aldehyde peak of intensity 0.20 or 0.25 like that. I have tried column, recrystallization, solvent wash but couldn't get the clean NMR of the final product.