How to download VCF mutation file for TCGA data in R studio?

In general, Journals Impact Factor is released in the fourth week of June every year?

Dear All,

Received an honorary nomination and invitation to join Sigma Xi, The Scientific Research Honor Society as a full member. Should I join this society? Is it a legit platform?

Please share your thoughts on this,

Regards,

Mahbub

I'm performing RNA-Seq data analysis for differentially expressed genes. as I'm new to this kind of work, so I performed these three gene count tools separately on the same bam file from RNA-Star alignment. I assumed that I might get a slite variation between these three tools' results. and I got some P-adj value variation for the same genes. so I want to know which one of these is better statiscally.

I am trying to extract DNA from a single insect from different sites. After extraction nanodrop readings show around 12-15 nanogram per microlitre concentration which is normal for the insect. However PCR amplification from two specific sites are failing everytime inspite of DNA being present. I have checked for presence of inhibitors by extracting DNA from four sites simultaneously which included the two sites mentioned above. DNA concentration is sufficient. However PCR amplification failed again. What could be the reasons for the failing PCRs?

I am attaching the gel image of the samples.

Many articles are suggesting CASP software to analyze the comets. Please share the details if there is any free access to this software or any other.

Thank you for coming by.

Can ImageJ or any such software be used to determine the area covered by fungal growth as in the image attached to this question? If yes, then how? I require this for antimicrobial assays. Kindly help and thanks in advance!

I have to use 15 pmol/10 pmol/5 pmol primers in my PCR protocol however I couldn't get how to dilute them. The stock concentration is 100 pmol.

Thank you

For example:

If there are 40 species identical between two sites, they are the same. However, two sites can each have 40 species each, but none in common. So by species number they are identical but by species composition they are 0% alike.

How can I calculate or show the species composition of the two sites over time?

Wondering if TrypLE Express cell dissociation agent is significantly better than other options for culturing mammalian cell lines. Currently mainly using Accutase (for fibroblasts and epithelial cells, NOT stem cells) and sometimes also trypsin for specific cell lines. Any positive/negative experience with TrypLE Express enzyme? Main attraction is that it does not require neutralization as trypsin does, but has longer incubation times. Would appreciate any comments and suggestions.