How to calculate components of overlapping peaks in chromatogram?

Instead of solving (deconvoluting) the peak, which would very tedious, why don't you improve the separation conditions e.g. buffers, column, etc. Can you post a picture of your chromatogram?

I agree with Farooq. Deconvoluting any type of peak requires some assumptions and one assumption is that your peak is very symmetrical. The most sure way of calculating the areas of coeluting components is to improve the resolution between the two peaks by doing some methods development on your particular separation, as mentioned by Farooq. However, there are two other ways that come to mind. The first one is, if the two components have different absorption maxima then you can monitor at two different wavelengths. That is, if component A absorbs at 200 nm and B doesn't absorb at 200 nm then you can monitor A without interfance from B. The other is if you have a calibration curve you can use linear algebra to calculate the concentrations but you will need to have a calibration curve at two different wavelength values for this to work (here is a link that will help you understand how to setup the equations https://facultystaff.richmond.edu/~rdominey/301/local/Multicomponent_uv_vis.pdf)

Okay...thank you...I attached the chromatogram......

Mr. Magaji,

Please find as attachment the math approaches. The first one is invilved in the AVALON algorithm, which is the TM of Thermo Fisher Company (http://www.thermoscientific.com/content/tfs/en/product/xcalibur-software.html)

The second approach we applied on chromatogram in the paper:

Molecular design, synthesis and physical properties of novel Cytisine-derivatives – Experimental and theoretical study, Journal of Molecular Structure, Volume 1034, 27 February 2013, Pages 173-182, Bojidarka Ivanova, Michael Spiteller

Due to the symmetry of the curve the direct Gauss fitting resulted to r2 > 0.9999. In the case of the asymmetric patternms than you may used the mixed function. The peak determination is obtained by deconvolution.

attachment to the chromatogram processing algorithms

NB! We still have not a correlation chemometric study between the math approaches for peak determination and integration of the patterns depending of the level of the overlapping, to evaluate the accuracy errors for analytical quantitation purpouses.

Dear USMAN

The best way is to improve the separation conditions, as they have mentioned before, but if you are still not able to do so, I would like to recommend you to use the MagicPlot software, they provide Student version for free, and it is easy to be used, I have tried it before to solve overlapping peaks in FTIR spectrum, and I got nice results, you can achieve that through Gaussian fitting, and also you will be able to perform base line correction, I can see from your attachment that you have a clear two or three maxima spectrum, anyway I did not try it for chromatorgrams, but in my opinion it is the same mathematical approach. Try to use it, just be sure that you can obtain XY data (ASC file), because you will need to re-plot your spectrum in the MagicPlot software. You can contact me if you need help. Try to download it from her:

http://magicplot.com/downloads.php

Do you know the chromatograms of the pure components of the mixture under study?

Yes....they gave a clear separation and distinct peaks......Thank you all for your immense contributions......

You can use the Least Squares Method: the solution of the linear equations.