How do I decide the size of the transwell insert for drug permeability assays?

I am a little confused about the nomenclature of the cell line referred to in the citations of ATCC -CRL 2467 as the BV-2 cell line. The BV-2 cell-line as described in ACCEGEN website is derived from C57BL/6 mouse. The CRL-2467 (EOC 2 in ATCC website) is from a C3H/Hej mouse. Can someone working with microglia cell-line clarify this.

Thanks

My gel always slants down towards the end. Even though the dye front doesn't slant, the proteins do. I'm not sure what to do.

I am writing a review paper. In my paper, can I use figures from "open access" journals/articles etc provided I cite them properly? (i.e. without have to go for requesting permission for using). Also, is there any way to obtain permission to use already published figures without incurring cost?

Thank you in advance for your answer.

How can Carboxymethyl cellulose (CMC) be dissolved in 60% Isopropanol to obtain a uniform solution?

Hi, I am working with proteinase K for a digestion. I am having trouble with the conversions to figure out how much exactly I need to use.

The label of my proteinase K bottle says 19mg/ml and company website says specific activity is 2u/mg. I need the final concentration to be 800u/ml. Not sure how to convert this. Any help is appreciated. Thanks.

Our lab is trying to grow human nasal epithelial cells (RPMI 2650) for a transepithelial resistance (TEER) assay. In the literature, epithelial cells are grown to confluency on transwells, then "raised" to the air-liquid interface. However, we have been growing our cells for over a month, but they never reached confluency. We tried to grow them at the air-liquid interface anyway by removing the medium from the apical side of the transwells, but the medium from the basolateral side would seep through, because the cells never formed a complete monolayer and instead grew in clumps. If anyone has worked with this cell line or with the TEER assay in general- is there something wrong with our setup? How do you grow cells at the air-liquid interface if they do not form a complete monolayer? Any advice would be much appreciated!

I am wanting to use a non-reducing sample buffer to resolve ubiquitin~E2 ligase interactions under various conditions by SDS-PAGE.

Looking at the literature some groups use SDS sample buffer to prepare their samples, whilst others use LDS sample buffer.

Why is this the case? Is there any significance to the cation or is it just user preference?

I designed a primer for vancomycin resistant gene Van A ,when I made PCR there was no result and all sample showed A primer dimer,so I guess that my primer design is incorrect ,how can i check this??