Does anyone know where I could get the VERO cell line (kidney cell line, cercopithecus aethiops) from? ATCC and Sigma can’t deliver in time.

Under conditions of:

Not feeling comfortable with the treatment decision?

Being diagnosed with a rare type of cancer?

Having several options for how to treat the cancer?

Not being able to see a cancer expert?

Cells are dying when density is increasing.

I want to produce stable cell lines of Huh7 and Huh7D which express functional TLR3.

I would like to label this TLR3 with BFP in order to save myself some time when It comes time to select colonies. 

I have made stable huh7.5 cell lines before, which express fluorescently labeled IPS1, so I am familiar with the process.

However, I do not want to affect TLR3 function and I don't want BFP to be cleaved (obvious concerns)

So, my question is: If possible, what terminus of TLR3 should I add BFP to? 

Or do they evolve ( gain or loose) depending of their micro environment?

Thank you for your inputs

i read about tumor marker and enjoy about its very important role in early diagnosis and help in assessment of therapy and recurrence probability,so i need to know if there is specific tumor marker for SCC effecting oral mucosa?

Should I use the same route of administration when comparing the effects of drugs on experimental animals?

Hello,

I have checked both mRNA and protein levels of alpha-fetoprotein in hepatocellular cancer cell line Huh7 and obtained 4-fold increase in mRNA, and 2-fold increase in protein levels. Now I'm wondering what could be the reason for lower protein amount, considering that afp is a secreted protein. Can anyone help me with the answer? How should I interpret this data?

What is the time taken to reach confluence for hepg2, huh7, heap 16 cell lines cultured in 10%FBS, DMEM high glucose