Does anyone know a software for automatic analysis of karyotypes ?

Hello everybody,

I'm a first year PhD student on evolutionary biology. As I work on the karyotyping of a species of earthworm, I would like to know if there is a software designed for this, cause sometimes I see chromosomes on my lames but impossible to count them.

Thanks alot.

Do anyone know a free software site for karyotyping analysis ?

How we can increase the intesity of Bands in GTG banding?

We work according to this procedure (Roneey, 2001), but our bands intensity are not good to distigush chromosomes by banding.

1.    Slides were putted in 50ml PBS solution in a coplin jar and incubated in 56C° waterbath for 8 min.

2.    Then, Slides rinsed thoroughly with distilled water.

3.    Slides Stained slides with working solution for 8min .

4.    Then, Slides Rinsed in a stream of distilled water and air dry.

5.    Finally, slides covered with DPX moutant and Coversliped in order to be ready under microscope.

Preparation of working staining solution:

For hot buffer trypsing G-banding procedure, a working staining solution prepared in the day of use by mixing the following reagents:

PBS buffer (pH 6.8) 26ml

Methanol 7.0ml

10X Trypsin-EDTA1.0ml

Giemsa stain solution 0.8ml

I keep getting the same answer when googling this question (which is the percentage in a population).

is there any difference between non-synonymous SNP and mutation?

and between synonymous SNP and silent mutation?

What's the difference between their effect on protein function?

I'd appreciate a reference on the topic.

thanks.

Hello All,

What is the expected spread amount on a single glass slide for a typical karyotyping procedure? Also, I know that a lot of the time it is hard to trace if all the chromosomes came from a single cell. So, how many of these spreads are usually useful.

I understand karyotyping is like an "art", so sometimes the process can be very subjective and hard to give an answer. However, I am just looking for an approximate number from someone that has experience with the process and has already done it many times.

Thank you so much!

What is the Giemsa Stain protocol utilized to prepare and analyze root metaphase chromosomes?

Thank you

What is the difference between melting temperature and annealing temperature?

Can anyone explain the step up PCR? The following is the paragraph, I could not understand the PCR condition for step up PCR

[For the DNA-dependent RNA polymerase II second largest subunit (RPB2), primer-pair RPB2-5F and RPB2-7Cr (Liu et al. 1999) was used with a step-up PCR that started with 5 cycles and annealing temperature of 48
C, followed by 5 cycles at 50
C and a final 25 cycles at 52
C. ]

Thanks

I need to concentrate my genomic DNA samples in a way that they will still be able to be used for a methylation assay. Some of the samples have really low yields, so I ideally need a method where you lose as little as possible. Thanks!