Question
Asked 25th Apr, 2016
CDNA synthesis kits and PCR for microRNA?
- Did anyone tried to see expression levels of microRNA using cDNA synthesis kit other than Qiagen?
As I read it's the best, since it polyadenylate miR before reverse transcription.
2.If I used miScript ll (Qiagen), Do I have to use SYBR green kit (Qiagen)? or can I use any other kit like Promega, ...etc?
Thank you.
All Answers (3)
By expression levels do you already know a subset of miRNAs that you're interested in or is this purely discovery and you're interested in global miRNA expression? A little greater detail will help others be able to offer better advice.
I've used Exiqon and Thermo/LIfeTechnologies kits. 1 benefit of the Exiqon miRcury Universal RT kit is just that. The universal RT step of this kit allows you to save valuable sample if interested in a large number of miRs. The LifeTech kit does have the option of a multiplex approach but it can get rather expensive. miRcury is sybr based and LifeTech is exonuclease probe; both have strengths and weaknesses.
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