The University of Manchester
Question
Asked 14th Dec, 2016
Are Extracellular vesicles (exosomes, microvesicles) are biologically stable after storage?
Am currently working on extracellular vesicle isolation from tissue culture conditioned media. I need those vesicles to be biologically active and sterile. that is why I am isolating them freshly every time I needed them,it is a really time consuming procedure. So I was wondering if storing them would actually affect their biological activity!! most of the work already done is for microscopical analysis, miRNA or protein isolation! but dose anyone knows if storing them would affect their biological activity, stability and viability? what is the best degree to store them at? do I re-suspend them with PBS or DMEM? and can I maintain the sterility if I filtered them with 0.45Microm filter?
Most recent answer
Hello Franco,
Thank you for your answer!!
All Answers (4)
University of Cincinnati
Vesicles are not "viable". One can store vesicles at -80 degree C in sterile PBS without destabilizing their contents.
University of Oslo
Hi Amna,
I also used to keep EVs at -80 °C in sterile PBS without calcium and magnesium. Anyway for functional assays I prefer to store them at 4°C for up to 1 week. Freezing reduces the size of microvesicles and likely affects the biological activity (freezing doesn't affect the size of exosomes).
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The University of Manchester
Hello Divaker,
I've got your point. I meant by viability is, will these vesicles continue doing their usual function? Thank you for your answer!!
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