Any advice on measuring phenolic content?

University of Hohenheim

Thanks again for the advice, have been working on it and diluting my samples seems to have made the reaction clearer. The reaction has a nice clear blue colour, with a PH of between 9 and 10

Chinaza Godswill Awuchi

University of Central Lancashire

Faride Noroozi shahri

Razi University

Alkasim Kabiru Yunusa

Kano University of Science & Technology

Abdelkader BOUAZIZ

SOUGUEUR Division - Chemistry (POLYMERS), Université Ibn Khaldoun Tiaret

Naser Jawad Kadhum

University Of Kufa

Agnieszka Gryszczyńska

Maybe, you have got too concentrate a plant extract solution or it isn`t clear. Try dissolve and filter through the filter paper a plant extract.

Dulce L. Ambriz-Pérez

Universidad Politécnica de Sinaloa

Hello Lucy, I also think that your samples could be very concentrate. In my lab, we use another proportions: 15 µl of extract, 240 µl destilled water, 15 µd folin and 30 µl · Na2CO3 4N, all these in a 96 well plate just like you and read at 725 nm. We base our metodology in Swain, T. y Hillis, W.E. 1959. and Singleton et al., 1999. Good luck!

https://www.researchgate.net/publication/267895134_Hepatoprotective_Activity_of_Guazoma_Tomentosa_Leaf_Extracts_Against_CCl_4_Induced_Liver_Damage_in_Rats

Dr. Shyam Baboo Prasad

Central Council for Research in Ayurvedic Sciences, Ministry of Ayush, Goverment of India

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Article Hepatoprotective Activity of Guazoma Tomentosa Leaf Extracts...

University of Hohenheim

Thank you so much, i will try to dilute my samples and see the reaction.

University of Hohenheim

Dulce, whats the concentration of your Na2co3.

University of Hohenheim

My leaf samples have been freeze dried, then i add 3ml of 80% methanol to 50mg sample, put in a roller then centrifuge. i use the methanol solution for analysis of phenolics.

Thamere Cheriet

Université de M'sila

try this one

298-JMES-1955-2015-Khadrao
ui.pdf
522.10 KB

University of Hohenheim

Thank you

Dulce L. Ambriz-Pérez

Universidad Politécnica de Sinaloa

Hi Lucy i use Na2CO3 4N, and disolve 100 mg of dried sample in 1 mL of methanol 80%.

Loai Basheer

Hebrew University of Jerusalem

Consider a reaction with Folin-Ciocalteu reagent (FCR). You can easily find the protocol in the web.

GOOD LUCK.

University of Hohenheim

Thanks again for the advice, have been working on it and diluting my samples seems to have made the reaction clearer. The reaction has a nice clear blue colour, with a PH of between 9 and 10

How can I calculate the total phenolic content of my sample (Plant Extract)?

Can anyone help me step wise calculation for determination of total phenolic contents in Plant extracts?

6 answers

Asked 20th Oct, 2017

Hammad Saleem

Given the following:

5mg/50ml (5mg plant extract was dissolved in 50ml Methanol) and 300 ul (microliter) was taken for analysis.

Absorbance of sample at 760 nm = 0.541

Standards curve provided the following information: y=0.0109x- 0.022; R2 = 0.9995.

Thanks.

What is the difference between extraction and isolation of essential oil or active substances from aromatic and medicinal plants?

Researchers have used these terms interchangeably while describing procedures of extraction or isolation of essential oils from aromatic plants and extracts of medicinal plants. Which of the two terms is correct for essential oils and extracts?

25 answers

Asked 11th Feb, 2015

B.R. Rajeswara Rao

Can anyone suggest the name or contact details of commercial laboratories offering chemical analysis of organic materials through LC-MS/MS?

I have plant methanol, ethyl acetate, n-hexane, and butanol extracts. I am interested in the chemical profiling of each extract through LC-MS/MS. If someone knows the details of commercial labs offering these facilities at reasonable prices then please the contact details. Thank you so much.

6 answers

Asked 24th Jan, 2020

Khalid Ali Khan

Yours truly,

Dr. Khalid

Hello respected Scientists: I am confused about the quantity of solvent used for extraction of phytochemicals. how much solvent we should use?

I am working on phytochemicals extractions. i am a little confused as to how much solvent we should use for the extraction? for example if i have 5 grams of powdered form of a sample then how much solvent i should in Soxhlet Apparatus for the extraction?

24 answers

Asked 20th Apr, 2016

Bilal Ahmad

How we can prepare different plant extracts for phytochemical analysis?

there are different methods available for extraction. Kindly suggest which one is best and why?

14 answers

Asked 24th Jan, 2015

Vinesh Kumar

Is there a readily available list of functional groups present in each phytochemical (alkaloids, flavanoids, resins, etc.)?

I have conducted phytochemical screening followed by FTIR for an aqueous plant extract and I want to know how to interpret the FTIR results to determine which phytochemicals are present based on the functional groups that have been determined/predicted by FTIR. Is there any other way to interpret the results?

3 answers

Asked 3rd Apr, 2024

Adarsh Shetty

How to characterize the crude extracts of plant sources?

I have extracted one plant souses with methanol and found it very potential in bioactivity (anti snake venom). The results are confirmed in both in vitro as well as in vivo. Further I found the possible components in the extract by GC-MS but when I communicate the reviewers suggested to characterize the extract to get the detailed information on extract composition. In this regard, I would like to know the techniques and methods to obtain the phytochemical information of crude plant extracts. Please share the knowledge in this aspects.

13 answers

Asked 30th May, 2014

Gopi Kadiyala

Does anywhere in Egypt perform the headspace technique?

I need a place in Egypt has a headspace apparatus to analyze traces of volatile oil or aroma of plants.

3 answers

Asked 28th Dec, 2014

Marwa Emad

What is the best solvent for caffeine and caffeic acid between water ethylacetate and methanol?