Actual structure of Hilyte Fluor 488 dye ??

Dear All,

The HPLC column was dropped from the top of HPLC machine to the table when it was connected to the instrument. It made the plastic connector broken and left the broken part in the column (it is still screwed in HPLC column). Does anyone know how to take that part out without destroying the column? Any suggestion or idea will be greatly appreciated. Thanks in advance!

Currently, I am running an HPLC analysis to quantify 4 active compounds in my natural product extract. However, due to a limited budget, I am only able to run 1 concentration of each of the 4 standard compounds. May I know, if I can still quantify the concentration of the 4 active compounds in my extract? As such, I am planning to ratio the peak surface area of the standard compounds with the peak surface area detected from my extract.

I have protein extracts from samples of testicular tissue from Wistar Rats, that were lyophilized contained RIPA buffer with protease inhibitors (150 mmol NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 0.8 mmol EDTA, 1.0 μg/mL aprotinin, 1.0 μg/mL leupeptin, and 35.0 μg/mL PMSF in 50 mmol Tris–HCl, pH 7.2). I’m using PBS at approximately pH 7.0, and it’s not completely reconstituting. I need it not to compromise the total protein dosage, for later Western Blotting and mas spectrometry analysis.

Hello,

I've been recently asked to contribute to a Scipod episode concerning publication of mine. In the past, I have received many emails from dubious publishers, seeking permission to revamp and publish previously published research, many of which are scams of various nature.

But what about Scipod?

So I'm trying to run a MaxQuant search for a purified, photolabeled protein and I set up everything in MaxQuant and it just closes right after I press start. I have verion 1.6.17.0. I've made sure my .raw and .fasta files are all in the same folder on my desktop which is something I know was an issue before. I'm using my own .fasta file (not a full proteome) so I don't know if it is something strange in the fasta. But it doesn't seem to matter what I change in my search parameters it still has the same problem. I'm working on a Dell and using Windows 10, if that helps. I've attached images and my mxqr file here. Any help would be greatly appreciated.

I want to do cartilage staining on Xenopus embryos (st 45). Following the protocol, I tried to dissolve 4 mg alcian blue in 7 ml ethanol plus 3 ml acetic acid. I found alcian blue powder is very hard to completely dissolved in this solution. Is there any suggestions?

I use graphpad to obtain the IC50 value, but the graphpad does not provide the SD for the IC50 (only the confidence interval). How can I calculate it? Thank you!

I want to know about the advantages of TCEP (tris(2-carboxyethyl)phosphine) over other reducing agents such as dithiothreitol (DTT) and β-mercaptoethanol.