AAV shelf life?

If ChR2 or NpHR3 is taken out of -80°C storage for a day and kept on ice, can the excess be stored for use the next day? Will I still get virus expression? And would I store it at 0°C or -80°C overnight?

AAV5-EF1a-DIO-eNpHR3.0-EYFP

AAV5-EF1a-DIO-hChR2(H134R)-EYFP

We had conducted few experiments on rats 2 months back. We perfused the rat with PBS followed by PFA. The brain was removed and coronally cut into two havles separating the cortex and cerebellum. Thereafterwe post fixed the brain in PFA for 24 hours .

We then trannsferred the brain to sucrose gradients and finally it was stored in 30% Sucrose at 4°C .

However the Abs required for IF failed to arrive on time and it will take a month more to arrive.

What should ideally be done?

Some people asked me not to make moulds in OCT and keep in - 80°C as that might lead to tissue shrinkage while others say the tissue is already "rotten" and is of no use?

How long (maximum) do you keep your BSA dilution at 4ºC?

Hey everyone,

Currently I am trying to decide whether to use a LoxP-STOP-LoxP cassette preceding a biosensor of interest or a FLEX system where my inverted biosensor is flanked by LoxP/Lox2272 sites. My main concern is the "leakiness" of the biosensor expressing in the absence of cre recombinase, as I am trying to get very specific expression of the biosensor in a set of neurons expressing the D2 receptor. Is one approach superior to the other in terms of fidelity of expression? Thank you in advance for any help!

I have 2 independent variables (genotype and treatment) and I want to analyze their correlation though sholl analysis. Which statistical test should I do?

1. “Resting Heart Rate, Heart Rate Variability and Electrocardiogram Changes In Chronic Alcoholics” was published in journal of medical sciences and clinical research volume 03 issue 02 February 2015

this is my article please verify

Hello Everyone,

the description of NEBuilder HiFi DNA Assembly mix says: "No PCR clean-up step required".

I always used this mix after DNA extraction from agarose gel , but now we have some problems with this step... Does anyone use this kit without PCR clean up, directly on the PCR product? Does it work? How many of the product is necessary? How could I measure or estimate the concentration of the DNA in this case?

Thank you very much,

Magdi

We want to know more about quality of VectorBuilder's services, because we want to order prepared AAV2retro-GDNF vector.