Mugla Üniversitesi

Wound healing, which is a complex and systematic process initiated by the body immediately after injury, is affected by many factors that can accelerate or delay this process. In this study, we aimed to evaluate the effects of dexmedetomidine on wound healing in rats. Our randomized controlled experimental study was conducted on a total of 16 male Wistar albino rats, divided into 8 control groups where the wound was covered only with sterile gauze and 8 experimental groups where dexmedetomidine was applied and then covered with sterile gauze and bandage. The wound areas of the rats were measured, histological evaluation and biochemical analysis were performed and, compared between groups. Our results showed that total antioxidant status (TAS) score was higher in the dexmedetomidine group and total oxidant status (TOS) score was higher in the control group. Interleukin-1, interleukin-6, tumor necrosis factor-α, hydroxyproline and epidermal growth factor scores of the groups did not show a statistically significant difference. Left lateral wound measurement of the dexmedetomidine group on the 1st and 3rd days was statistically wider than the left lateral wound measurement of the control group. There was a statistically significant decrease in wound sizes in all groups and on days 1-3-7-9.-12-15.-17.-21. The dexmedetomidine group had more pronounced reepithelialization and more intense collagen deposition compared to the control group. Based on our results, we concluded that dexmedetomidine may have an improving role in the wound healing process but more comprehensive studies are needed to evaluate the effects of dexmedetomidine on wound healing.

The axolotl (Ambystoma mexicanum) is renowned for its remarkable regenerative capabilities, which are not diminished by the transition from a neotenic to a metamorphic state. This study explored the microbiome dynamics in axolotl limb regeneration by examining the microbial communities present in neotenic and metamorphic axolotls at two critical stages of limb regeneration: pre-amputation and during blastema formation. Utilizing 16S rRNA amplicon sequencing, we investigated the variations in microbiome profiles associated with different developmental and regenerative states. Our findings reveal a distinct separation in the microbiome profiles of neotenic and metamorphic samples, with a clear demarcation in microbial composition at both the phylum and genus levels. In neotenic 0DPA samples, Proteobacteria and Firmicutes were the most abundant, whereas in neotenic 7DPA samples, Proteobacteria and Bacteroidetes dominated. Conversely, metamorphic samples displayed a higher abundance of Firmicutes and Bacteroidetes at 0DPA and Proteobacteria and Firmicutes at 7DPA. Alpha and beta diversity analyses, along with dendrogram construction, demonstrated significant variations within and between the sample groups, suggesting a strong influence of both developmental stage and regenerative state on the microbiome. Notably, Flavobacterium and Undibacterium emerged as distinctive microbial entities in neotenic 7DPA samples, highlighting potential key players in the microbial ecology of regeneration. These findings suggest that the axolotl’s microbiome is dynamically responsive to blastema formation, and they underscore the potential influence of microbial communities on the regeneration process. This study lays the groundwork for future research into the mechanisms by which the microbiome may modulate regenerative capacity.

Data analytics provide an important contribution to improving educational processes, as well as managerial and organisational processes in higher education. They are also significant in transforming behavioural, performance, and interaction data on digital learning platforms into pivotal information about the learning process. This chapter presents analysis results from instructional and behavioural formative assessment data for an online common course stored on a Turkish state university’s learning management system using cluster analysis. This chapter focuses on evaluating activity and assessment data to discover student groups as an important input of assessment analytics. Developing supervised models require a priori labels, such as individual student success or fail scores, and a multidimensional dataset identifying relevant performance information gathered during the learning process. In a more realistic setting, the labels are not fully known until the semester end and supervised learning models may then create learning bias when trained only using assessment data. Therefore, cluster analysis presents a promising approach through observing performance similarities between students not yet evaluated for a course or subjected to end-of-term assessment. Although feedback mechanisms were not studied in this chapter, this approach can further help achieve necessary steps to increase student success before a course ends. This study grouped students based on learning performance data using cluster analysis, with models developed and evaluated according to various performance indicators. The main concepts related to the function of the methods in assessment analytics are discussed, and descriptive results obtained through the model are shared and evaluated for a specific case.

Purpose Imposter Syndrome(IS) is defined as “the inability to internalize achievement and a tendency to attribute success to external factors such as luck, error, or personal connections. The study aimed to evaluate the factors associated with IS and its association with burnout(BO) in medical oncologists (MOs). Methods A cross-sectional study was conducted focusing on the MOs actively working in the field. The IS scores (ISS) was tested using the Clance imposter phenomenon Scale and BO was tested by using the Maslach burnout inventory. The subscales of BO; emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA) were analyzed. The characteristics associated with IS and BO were tested. Results In the study, 417 MOs were evaluated. The mean of ISS was 50.8+/-15.3, and 122(29.3%) participants had high ISS. The multivariate analysis revealed that female gender was the only factor associated with high ISS (OR: 1.85, CI95%, 1.1–2.9, p = 0.009). While there was a strong positive correlation between ISS and EE (r = 0.54, p < 0.001) and DP (r = 0.48, p < 0.001), a strong and negative correlation was found between ISS and PA score (r= -0.41, p < 0.001). High ISS score was the only statistically significant parameter associated with a high EE score, high DP scores and low PA scores. Conclusion The results of the study indicated that IS is prevalent in MOs. The MOs with IS were more emotionally exhausted and more depersonalized. In addition, they had less feeling of personal accomplishment. The IS and its consequences on MOs should be further studied and the strategies to cope with IS should be developed.

Objectives In congenital hemolytic anemias (CHA), it is not always possible to determine the specific diagnosis by evaluating clinical findings and conventional laboratory tests. The aim of this study is to evaluate the utility of next‐generation sequencing (NGS) and clinical‐exome‐based copy number variant (CNV) analysis in patients with CHA. Methods One hundred and forty‐three CHA cases from 115 unrelated families referred for molecular analysis were enrolled in the study. Molecular analysis was performed using two different clinical exome panels in 130 patients, and whole‐exome sequencing in nine patients. Exome‐based CNV calling was incorporated into the traditional single‐nucleotide variant and small insertion/deletion analysis pipeline for NGS data in 92 cases. In four patients from the same family, the PK Gypsy variant was investigated using long‐range polymerase chain reaction. Results Molecular diagnosis was established in 86% of the study group. The most frequently mutated genes were SPTB (31.7%) and PKLR (28.5%). CNV analysis of 92 cases revealed that three patients had different sizes of large deletions in the SPTB and six patients had a deletion in the PKLR. Conclusions In this study, NGS provided a high molecular diagnostic rate in cases with rare CHA. Analysis of the CNVs contributed to the diagnostic success.

Disease outbreaks negatively affect fish production. Antimicrobial agents used in the treatment of diseases become ineffective over time because of antibiotic resistance developed by bacteria distributed in the aquaculture environment. This study was conducted for 4 months (cold period) in a fish farm to detect the fish disease, cold water streptococcosis. In the study, four brood stock showing disease signs were detected. Bacteria isolates were obtained and identified as Vagococcus salmoninarum . Antimicrobial susceptibility of V. salmoninarum was tested and antibiotic resistance gene profiles of V. salmoninarum isolates were screened. The phylogenetic relation of the isolates with the previously reported strains was evaluated. Antibiotic resistance developed by pathogenic bacteria is distributed in the aquaculture environment. The transfer of resistance genes from one bacterium to another is very common. This situation causes the antimicrobial agents used in the treatment of diseases to become ineffective over time. The disc diffusion test showed that all four isolates developed resistance to 13 (FFC30, AX25, C30, E15, CF30, L2, OX1, S10, T30, CRO30, CC2, PT15 and TY15) of the evaluated antibiotics and were about to develop resistance to six others (AM 10, FM 300, CFP75, SXT25, APR15 and TE30). Furthermore, antibiotic resistance genes tetA , sul1 , sul2 , sul3 , dhfr1 , ereB and floR were detected in the isolated strain. Moreover, the phylogenetic analysis showed that isolated V. salmoninarum strain (ESN1) was closely related to the bacterial strains isolated from USA and Jura.

Background Refugees have poorer bereavement outcomes due to their previous trauma-related experiences. However, there is limited evidence on their bereavement care needs and risk factors from a psycho-socio-cultural viewpoint. Objective To synthesise evidence concerning bereavement support for refugees in the context of psycho-socio-cultural concept of personhood. Methods We conducted a rapid systematic review with a narrative synthesis of results using the Ring Theory of Personhood. Five electronic databases and grey literature were used to identify the relevant qualitative, quantitative, and mixed methods studies related to bereavement experiences of adult refugees who had traumatic experiences and close-one death. Findings were grouped into (i) four rings (innate, individual, relational, and societal) of personhood and (ii) bereavement support needs of refugees. Quality was assessed using the appraisal tool developed by Hawker et al (2002). Results 11 studies (8 quantitative, 2 mixed-methods, 1 qualitative) were identified. 55% of refugees were male (n=1674 out of 3053). 20% of bereaved refugees had prolonged grief disorder. Based on the rings of personhood, various risk factors were associated with bereavement such as female sex, age, comorbidity, culture and religious beliefs and values, and social factors. They needed psycho-socio-cultural bereavement support interventions which may increase healthcare providers’ insight into refugees’ perspectives, values and preferences. Conclusions To facilitate higher quality person-centred care and provide more positive bereavement outcomes, we recommend culturally adapted and personalised bereavement care for bereaved refugees from diverse backgrounds. The findings may provide important insights into cultural processes shaping the bereavement response to healthcare services and help healthcare professionals to respond more appropriately and sensitively to individuals’ needs. Further research is needed to identify useful psycho-socio-cultural assessment and intervention strategies aiming at the early detection and reduction of bereavement-related risk factors in this specific population. PROSPERO-ID CRD42022370126

Objectives 1) Culture Mycobacterium avium ssp. paratuberculosis (MAP)from blood, 2) assess infection persistence, 3) determine Crohn’s disease (CD) cytokine expression, 4) compare CD cytokine expression to tuberculosis, and 5) perform a meta-analysis of cytokine expression in CD. Methods The Temple University/Abilene Christian University (TU/ACU) study had a prospective case control design with 201 subjects including 61 CD patients and 140 non-CD controls. The culture methods included MGIT, TiKa and Pozzato broths, and were deemed MAP positive, if IS900 PCR positive. A phage amplification assay was also performed to detect MAP. Cytokine analysis of the TU/ACU samples was performed using Simple Plex cytokine reagents on the Ella ELISA system. Statistical analyses were done after log transformation using the R software package. The meta-analysis combined three studies. Results Most subjects had MAP positive blood cultures by one or more methods in 3 laboratories. In our cytokine study comparing CD to non-CD controls, IL-17, IFNγ and TNFα were significantly increased in CD, but IL-2, IL-5, IL-10 and GM-CSF were not increased. In the meta-analysis, IL-6, IL-8 and IL-12 were significantly increased in the CD patients. Conclusion Most subjects in our sample had MAP infection and 8 of 9 subjects remained MAP positive one year later indicating persistent infection. While not identical, cytokine expression patterns in MAP culture positive CD patients in the TU/ACU study showed similarities (increased IL-17, IFNγ and TNFα) to patterns of patients with Tuberculosis in other studies, indicating the possibilities of similar mechanisms of pathogen infection and potential strategies for treatment.

Background Glioblastoma multiforme (GBM) ranks among the prevalent neoplastic diseases globally, presenting substantial challenges in therapeutic management. Traditional modalities, encompassing surgical excision, chemotherapy, and radiation therapy, have yielded suboptimal response rates in GBM due to its intrinsic pathological resistance. This underscores the imperative for identifying novel molecular targets to enhance therapeutic efficacy. Literature indicates a notable correlation between androgen receptor (AR) signaling pathways and GBM pathogenesis. Consequently, to mitigate the adverse effects associated with androgenic modulation of AR, the scientific community has pivoted towards the synthesis of non-steroidal anabolic agents known as selective androgen receptor modulators (SARMs). Among these, S4, a relatively unexplored SARM with favorable oral bioavailability has emerged as a candidate of interest. Methods and Results This investigation substantiates the anti-oncogenic potential of S4 in both temozolomide-responsive and -resistant GBM cellular models through comprehensive cellular and molecular evaluations. We observed a marked restriction in GBM cell viability, growth, and motility, coupled with an induction of apoptotic pathways, reactive oxygen species (ROS) generation, and cellular senescence. Additionally, S4 exposure precipitated the upregulation of genes associated with apoptosis, cell-cycle arrest, DNA damage response, and senescence, while concurrently downregulating those involved in cellular proliferation. Conclusion Future research endeavors are warranted to delineate the precise mechanisms underpinning S4's actions, assess its antineoplastic effects in vivo, and evaluate its ability to penetrate the blood-brain barrier.

Simple Summary TCGAnalyzeR provides a novel web site integrating 123 pre-computed pan-cancer cohorts (i.e., microsatellite instability, immune, metastasis, PAM50, Triple Negative breast cancer, idh1-mutated glioblastoma, etc.), along with our own iCluster+ subcohorts, computed based on pre-processed single-nucleotide variations, copy number variations, differential expression, miRNA, methylation, and clinical data. TCGAnalyzeR interface provides an optimized and fully customizable experience to each user, enabling the selection of their own “My patients” or “My genes” to a clipboard. Several use cases of the web site are presented as Help documents. Abstract For humans, the parallel processing capability of visual recognition allows for faster comprehension of complex scenes and patterns. This is essential, especially for clinicians interpreting big data for whom the visualization tools play an even more vital role in transforming raw big data into clinical decision making by managing the inherent complexity and monitoring patterns interactively in real time. The Cancer Genome Atlas (TCGA) database’s size and data variety challenge the effective utilization of this valuable resource by clinicians and biologists. We re-analyzed the five molecular data types, i.e., mutation, transcriptome profile, copy number variation, miRNA, and methylation data, of ~11,000 cancer patients with all 33 cancer types and integrated the existing TCGA patient cohorts from the literature into a free and efficient web application: TCGAnalyzeR. TCGAnalyzeR provides an integrative visualization of pre-analyzed TCGA data with several novel modules: (i) simple nucleotide variations with driver prediction; (ii) recurrent copy number alterations; (iii) differential expression in tumor versus normal, with pathway and the survival analysis; (iv) TCGA clinical data including metastasis and survival analysis; (v) external subcohorts from the literature, curatedTCGAData, and BiocOncoTK R packages; (vi) internal patient clusters determined using an iClusterPlus R package or signature-based expression analysis of five molecular data types. TCGAnalyzeR integrated the multi-omics, pan-cancer TCGA with ~120 subcohorts from the literature along with clipboard panels, thus allowing users to create their own subcohorts, compare against existing external subcohorts (MSI, Immune, PAM50, Triple Negative, IDH1, miRNA, metastasis, etc.) along with our internal patient clusters, and visualize cohort-centric or gene-centric results interactively using TCGAnalyzeR.

Socioscientific issues (SSI), important for developing scientific literacy, are controversial issues related to social and scientific information. This study investigated the effect of argumentation about SSI on middle school students’ views, attitudes, knowledge about SSI, and their scientific literacy. The study uses a pre-test and post-test control group design with a sample of 49 seventh-grade students. The experimental group used SSI-based instruction with argumentation, while the control group used SSI-based instruction. The data collection instruments used were the scientific literacy scale, the open-ended questions on scientific literacy, the views and attitudes towards SSI scale, and the open-ended questions on SSI knowledge. The research findings indicate a difference in the pre-test and post-test scientific literacy scores between the experimental and control groups. The experimental group did not show a statistically significant difference in views and attitudes to SSI between the pre and post-tests. However, the control group showed a statistically significant difference in Favor of the post-test compared to the pre-test. The SSI knowledge questions showed an increase in both groups because of the interventions. It is recommended that SSI-based instruction with argumentation assists learners in recognising diverse viewpoints, researching existing issues in society, and making decisions regarding the ethical, environmental, and financial effects of them. It is, therefore, recommended that science education programmes more readily incorporate socio-scientific issues.

The physical, mechanical, and thermal properties of Oriental beech (Fagus orientalis), which had been impregnated with the water-based, copper-containing Korasit KS material from the Alkaline Copper Quaternary group, were investigated in this study. According to ASTM 1413-07el (2007) standard, the wood samples used in the investigation were impregnated with 3 % and 6 % aqueous solutions of Korasit KS. The modulus of rupture, thermal, and water absorption tests were performed on samples of Oriental beech after they had been impregnated. Oriental beech's modulus of rupture values decreased as a result of Korasit KS impregnation. Additionally, Oriental beech had lower modulus of rupture values at greater concentrations of Korasit KS. In every water absorption period, the water absorption values of the Oriental beech impregnated with Korasit KS were higher than those of the control group. Our results showed that Korasit KS impregnation enhanced thermal properties of Oriental beech. Moreover, higher concentration levels of Oriental beech yielded better thermal characteristics of Oriental beech.

Sağlık kurumlarının kuruluş amacı hedef kitlesinde bulunan hastalara sağlık hizmeti sunmaktır. Sağlık kurumlarının çevrelerinde olumlu olarak tanınması ve varlığını sürdürebilmesi, hizmet verdiği hastalarda memnuniyet yaratması ile mümkündür. Bu çalışmanın amacı, hasta deneyimleri sonucunda kurumlar hakkında edinilen izlenimlerle oluşan kurumsal imajın hasta memnuniyeti üzerindeki etkisinin ortaya konmasıdır. Araştırma, ilişkisel tarama modeli ile yapılmış olup ayrıca kesitsel nitelik taşımaktadır. İstanbul ilinde özel hastanelerden hizmet almış kişiler üzerinde yapılan bu çalışmada, araştırma verilerinin elde edilmesinde anket yöntemi uygulanmıştır. Araştırma kapsamında 446 katılımcıdan elde edilen verilere göre kurumsal imaj ve hasta memnuniyeti arasında yüksek düzeyde korelasyon bulunduğu ve kurumsal imajın hasta memnuniyeti üzerinde anlamlı ve pozitif yönde etkisinin bulunduğu belirlenmiştir (β=1,171, p<0,05). Aynı zamanda değişkenler arasındaki yüksek korelasyon hem kurumsal imajın hem de hasta memnuniyetinin birbirini destekleyen iki değişken olduğunu göstermektedir. Sektörde rekabet avantajı sağlamak ve sürdürülebilir rekabet üstünlüğü elde etmek isteyen sağlık kurumlarına kurumsal imaj çalışmalarına önem vermeleri ve kurumsal imajlarını pozitif yönde geliştirmeleri önerilmektedir.

Phenolic acids are derivatives of benzoic and cinnamic acids, which possess important biological activities at certain concentrations. Trans-cinnamic acid (t-CA) and its derivatives, such as p-coumaric acid (p-CA) and ferulic acid (FA) have been shown to have antibacterial activity against various Gram-positive and -negative bacteria. However, there is limited information available concerning the antibacterial mode of action of these phenolic acids. In this study, we aimed to ascertain metabolic alterations associated with exposure to t-CA, p-CA, and FA in Escherichia coli BW25113 using a nuclear magnetic resonance (NMR)-based metabolomics approach. The results showed that t-CA, p-CA, and FA treatments led to significant changes (p < 0.05) in the concentration of 42, 55, and 74% of the identified metabolites in E. coli, respectively. Partial least-squares discriminant analysis (PLS-DA) revealed a clear separation between control and phenolic acid groups with regard to metabolic response. Moreover, it was found that FA and p-CA treatment groups were clustered closely together but separated from the t-CA treatment group. Arginine, putrescine, cadaverine, galactose, and sucrose had the greatest impact on group differentiation. Quantitative pathway analysis demonstrated that arginine and proline, pyrimidine, glutathione, and galactose metabolisms, as well as aminoacyl-tRNA and arginine biosyntheses, were markedly affected by all phenolic acids. Finally, the H2O2 content of E. coli cells was significantly increased in response to t-CA and p-CA whereas all phenolic acids caused a dramatic increase in the number of apurinic/apyrimidinic sites. Overall, this study suggests that the metabolic response of E. coli cells to t-CA is relatively different from that to p-CA and FA. However, all phenolic acids had a certain impact on oxidative/antioxidant status, genomic stability, arginine-related pathways, and nucleic acid metabolism.

Improvement techniques aimed at enhancing the dielectric strength and minimizing the dielectric loss of insulation materials have piqued the interest of many researchers. It is worth noting that the electrical breakdown traits of insulation material are determined by their electrochemical and mechanical performance. Possible good mechanical, electrical, and chemical properties of new materials are considered during the generation process. Thermoplastic polyurethane (TPU) is often used as a high-voltage insulator due to its favorable mechanical properties, high insulation resistance, lightweight qualities, recovery, large actuation strain, and cost-effectiveness. The elastomer structure of thermoplastic polyurethane (TPU) enables its application in a broad range of high-voltage (HV) insulation systems. This study aims to evaluate the feasibility of using TPU on transformer windings as a solid insulator instead of pressboards. The investigation conducted through experiments sheds light on the potential of TPU in expanding the range of insulating materials for HV transformers. Transformers play a crucial role in HV systems, hence the selection of suitable materials like cellulose and polyurethane is of utmost importance. This study involved the preparation of an experimental setup in the laboratory. Breakdown tests were conducted by generating a non-uniform electric field using a needle–plane electrode configuration in a test chamber filled with mineral oil. Various voltages ranging from 14.4 kV to 25.2 kV were applied to induce electric field stress with a step rise of 3.6 kV. The partial discharges and peak numbers were measured based on the predetermined threshold values. The study investigated and compared the behaviors of two solid insulating materials under differing non-electric field stress conditions. Harmonic component analysis was utilized to observe the differences between the two materials. Notably, at 21.6 kV and 25.2 kV, polyurethane demonstrated superior performance compared to pressboard with regards to the threshold value of leakage current.

Introduction:Human epidermal growth factor receptor 2 (HER2) overexpression is a characteristic observed in 18%–20% of all breast cancer cases. While some clinics have adopted abbreviated neoadjuvant treatment for HER2-positive breast cancer, there remains a shortage of comprehensive clinical data to support this practice. One of the primary considerations in the treatment of HER2-positive breast cancer is the need for regimens that minimize cardiac toxicity while not compromising survival outcomes. This issue continues to be a topic of discussion within the medical community. Methods:Retrospectively analyzed were the data of HER2-positive breast cancer patients, aged ≤65 years, with left ventricular ejection fraction (LVEF) ≥50% who received neoadjuvant chemotherapy and underwent surgery at 10 different oncology centers in Türkiye between October 2016 and December 2022. Results: A total of 142 patients were included in the study. The treatment arms were divided into 4 to 6 cycles of docetaxel/trastuzumab/pertuzumab (DTP) for arm A, 4 cycles of adriamycin/cyclophosphamide (AC) followed by 4 cycles of taxane/TP for arm B. There were 50 patients (35.2%) in arm A and 92 patients (64.8%) in arm B. The mean age of the patients in arm A was 53 years (±11, SD), while in arm B it was 48 years (±11, SD). The median follow-up of all of the patients was 19.9 months (95% CI: 17.5–22.3). Median disease-free survival (DFS) was not reached in either treatment arm. The 3-year DFS rates for treatment arms A and B were 90.0% and 83.8%, respectively, and the survival outcomes between the groups were similar (p = 0.34). Furthermore, the pathologic complete response (pCR) rates were similar in both treatment arms, at 50.0% and 51.1%, respectively (p = 0.90). During the treatment period, the LVEF decline was seen in 3 patients (6%) in arm A and 11 patients (12%) in arm B, but the difference between the groups did not reach statistical significance (p = 0.37). Although the LVEF decreases were asymptomatic in both treatment arms, the rate of LVEF decrease was between 11% and 20% in 2 patients (2.2%) in arm B. Discussion/Conclusion: This study supports shortened neoadjuvant treatment of HER2-positive breast cancer, a common practice in some clinics. Given the similar DFS and pCR rates between DTP and AC-taxan/TPtreatments, DTP may avoid the severe toxicities associated with standard combined chemotherapy regimens.

This work examined the changes in surface characteristics of Oriental beech (Fagus orientalis L.) covered with vegetable oil-based epoxide-amine (ETO + D230) and its nanocomposite derivatives Epoxy + amine + carbon nanotube (ETO + D230 + C), Epoxy + amine + fullerene (ETO + D230 + F), and Epoxy + amine + graphene (ETO + D230 + G) doped with carbon nanoparticles following weathering. Results showed that while all coated groups displayed good lightness stability following weathering, the non-coated (control) group produced negative lightness stability values. The coated Oriental beech had greater overall color stability than the uncoated (control) group. The specimens coated with ETO + D230 + C nanocomposite exhibited the best color stability. All uncoated and coated specimens produced negative a* and positive b* values, indicating a propensity to turn greenish or yellowish after weathering, respectively. After weathering, while the surface roughness values of all specimens increased, their gloss decreased. In addition, after weathering, all coated samples had less gloss loss and lower surface roughness than the control group. Our findings show that following weathering, Oriental beech coated with ETO + D230 + C, ETO + D230 + F, and ETO + D230 + G nanocomposites have better surface properties than ETO + D230-coated Oriental beech.

Solitary functioning kidney (SFK) can be defined as the absence or hypofunction of a kidney due to acquired or congenital reasons. Congenital solitary functioning kidney (cSFK) is more common than acquired (aSFK) and is characterized by the anatomical absence (agenesis) or hypofunction (hypoplasia, hypodysplasia) of one kidney from birth. Among the acquired causes, the most im-portant is nephrectomy (Nx) (due to donor, trauma or mass resection). Patients with SFK are at risk for the development of chronic kidney disease (CKD) in the long term. This risk potential is also significantly affected by hypertension. The relationship between hypertension and subclinical chronic inflammation is a connection that has not yet been fully clarified pathogenetically, but there are many studies highlighting this association. In recent years, studies examining different fibrosis and inflammation biomarkers in terms of the evaluation and prediction of renal risks have become increasingly popular in the literature. Oxidative stress is known to play an important role in homocysteine-induced endothelial dysfunction and has been associated with hypertension. In our study, we aimed to investigate the relationship between ambulatory blood pressure moni-toring (ABPM) and urinary/serum fibrosis and inflammatory markers in patients with SFK. We prospectively investigated the relationship between ABPM results and soluble urokinase plas-minogen activator receptor (suPAR), procollagen type III N-terminal peptide (PIIINP), homocys-teine and other variables in 85 patients with SFK and compared them between cSFK and aSFK groups. In the etiology of SFK, congenital or acquired origin may differ in terms of the signifi-cance of biomarkers. In particular, the serum homocysteine level may be associated with different clinical outcomes in patients with cSFK and aSFK.

Introduction: Klebsiella pneumonia causes serious infections in hospitalized patients. In recent years, carbapenem-resistant infections increased in the world. The molecular epidemiological investigation of carbapenem-resistant K. pneumoniae isolates was aimed in this study. Methodology: Fifty carbapenem-resistant K. pneumoniae isolates from six geographical regions of Turkey between September 2019-2020 were included in the study. The disk diffusion method was used for the antibiotic susceptibility testing. The microdilution confirmed colistin susceptibility. Genetic diversity was investigated by MLST (Multi-Locus Sequence Typing). Results: The resistance rates were as follows: 49 (98%) for meropenem, 47 (94%) imipenem, 50 (100%) ertapenem, 30 (60%) colistin and amoxicillin-clavulanate, 49 (98%) ceftriaxone, 48 (96%) cefepime, 50 (100%) piperacillin-tazobactam, 47 (94%) ciprofloxacin, 40 (80%) amikacin, 37 (74%) gentamicin. An isolate resistant to colistin by disk diffusion was found as susceptible to microdilution. ST 2096 was the most common (n:16) sequence type by MLST. ST 101 (n:7), ST14 (n:6), ST 147 and ST 15 (n:4), ST391 (n:3), ST 377 and ST16 (n:2), ST22, ST 307, ST 985, ST 336, ST 345, and ST 3681 (n:1) were classified in other isolates. In İstanbul and Ankara ST2096 was common. Among Turkey isolates, the most common clonal complexes (CC) were CC14 (n:26) and CC11 (n = 7). Conclusions: In Turkey, a polyclonal population of CC14 throughout the country and inter-hospital spread were indicated. The use of molecular typing tools will highlight understanding the transmission dynamics.