When optimizing CAR T cell therapy to address everyday challenges in cancer treatment, multianalyte assays bring forth several advantages:
Fast and accurate quantitation that's crucial for timely intervention
Small sample size requirement (25 μL) that enables detailed molecular response characterization
Triplicate readings and pre-loaded standard curves for high-quality, reproducible data
Get closer to understanding the interactions between T cell activation, cytokine release, and patients' unique molecular responses. Download the complete poster to learn more.

Conduct your next experiment with confidence. Get the knowledge you need to ensure reproducible results and avoid false positives in the latest edition of our ELISA Guide.
Download the guide now to learn about:
The advantages & disadvantages of each ELISA type
Use cases for different ELISA formats
The importance of ELISA controls
Best practices for data analysis

Simple Western simplifies assay development and provides scalable automation, size resolution, and reproducible quantitation with advantages over other protein analysis methods such as ELISA and Flow Cytometry. Here we present a concise scientific review of patents and publications that showcase how Simple Western is driving innovation of new CAR T-cell technology.

In this application note, we use TcBuster™, Bio-Techne’s novel non-viral gene delivery technology, to generate CD19 CAR T cells and validate the use of Ella as a T Cell activation marker assay platform.
We have demonstrated that Simple Plex multianalyte assays provide high-quality, quantitative data in just 90 minutes with unparalleled flexibility and ease of use.
Read on to learn more.

Adeno-associated virus (AAV) is a commonly used viral vector in gene transduction workflows because it is known to readily infect a variety of cell types.
In this application note, we pair Simple Plex™ microfluidic cartridge assays on Ella™ with PROGEN antibodies and demonstrate utility for accurate and efficient AAV capsid titer determination. Read on to learn more.

Bio-Techne offers a wide selection of products to support stem cell and organoid-related workflows, including Cultrex™ basement membrane extracts, cytokines and growth factors, small molecules, media supplements, antibodies and RNAScope™ In Situ Hybridization assays. Please find the organoid-related resources that we offer listed below.

This eBook offers a great resource on the background, current state, and future use of cell-based model systems for disease modeling, drug screening, and toxicology. Included are chapters on primary cells, cell lines, stem cells, 3-D cell culture models, and more complex tissue and organ modeling systems.

The following resources are included in the download;
Evolution of Cell Culture
This eBook provides a primer on the history, current state, and future use of cell-based model systems for drug screening and toxicology.
Organ-on-a-Chip Technology
This whitepaper highlights the progress, challenges, and the exciting future of this innovative technology.
Organoid Wall Poster
This poster shows some of the visually-stunning organoid images that have been generated by Cell and Gene Therapy scientists at Bio-Techne.
The Organoid Handbook
This handbook highlights some of the notable publications that have used organoids to investigate questions about development, disease, or drug safety.

Organoids and 3-D cell culture systems are emerging as powerful new platforms for exploring human organ development, modeling disease, screening for drug toxicity, and investigating personalized medicine. This poster shows some of the visually-stunning organoid images that have been generated by Cell and Gene Therapy scientists at Bio-Techne.

Tumor cells frequently exploit the activities of immune checkpoint molecules by overexpressing ligands that activate inhibitory immune checkpoint receptors, which allows them to evade destruction by the host’s immune system. As a result, researchers have focused on targeting immune checkpoint molecules for cancer immunotherapy using either agonists of immune stimulatory receptors or antagonists of immune inhibitory receptors.
This eBook presents some current and emerging immune checkpoint molecules being investigated as potential targets for cancer immunotherapy. Some of the products we offer for studying these molecules are also presented.

Interrogation of gene expression in complex, highly heterogeneous tissue, such as the brain, is an ongoing challenge due to the multiple cell types and states that comprise these tissues.
Single-cell RNA sequencing (scRNA-seq) is currently one of the most insightful tools to understand complex tissue structure and function. However, the dissociation of cells during the method results in loss of important information about the spatial organisation of cells within tissue.
The RNAscope technology is a highly specific, sensitive, and multiplexed RNA in situ hybridization methodology that allows researchers to address these contextual challenges through spatial mapping of RNA targets with single cell resolution. Through the interrogation of tissue samples with sequence-specific probes users can:
Visualize the cellular heterogeneity of complex organs with multiplexing and spatial capabilities
Spatially map and confirm scRNA-seq gene profiles at the single cell level in the tissue context
Localize newly identified cell subtypes and cell markers
Explore how the multiplex capabilities of the RNAscope assays can be applied to your neuroscience research and scRNA-seq workflows with our new Application Note – “The RNAscope multiplex in situ hybridization technology enables the incorporation of spatial mapping and confirmation of gene signatures into single cell RNA sequencing workflows”

Cell & Gene Therapies (C&GT) are a promising, rapidly growing area of research, and ProteinSimple is here to help you get safe and effective treatments to the people who need them.
ProteinSimple’s analytical platforms give you the automation and scalability needed for the development and manufacturing of C&GT products, and with low volume requirements, we help you preserve these precious samples. Platform methods can be seamlessly transferred across labs and project phases, from discovery to manufacturing, giving you consistent results from start to finish.
Download the eBook to learn more.

Miniaturizing organs to the size of an AA battery may once have sounded like a prospect confined to the realms of science fiction. But, with organ-on-a-chip technologies seeing breakthrough after breakthrough, this concept has now become a reality – and without the use of a super shrink ray.
Organ-on-a-chip, an innovative technology developed over the past decade, can mimic the physiological environment and functionality of human organs on a tiny microfluidic device. They show great potential to revolutionize drug discovery and development, personalized medicine as well as drive forward the study of disease modelling.
Download our whitepaper to learn more.

An organoid is a miniaturized version of an organ produced in vitro that shows realistic micro-anatomy, is capable of self-renewal and self-organization, and exhibits similar functionality as the tissue of origin.
Organoids are model systems that, in conjunction with advances in cell reprogramming technology and gene editing methods, allow unprecedented insight into human development, disease modeling, drug screening, and transplantation.
Download the handbook to learn more.

Achieve reliable and reproducible results including troubleshooting tips with our step-by-step guide.
Whether you are a student/researcher new to western blotting, or a skilled scientist, you’ll gain valuable insights to help you to master the technique.
You'll learn:
Main principles of western blotting
Troubleshooting tips
How to accurately analyze data
Proper controls, antibody validation, and data normalization
Fully automated Simple Western™ Platform from ProteinSimple

The current most used tools for miRNA detection rely on methods such as RNA-Seq and qPCR, which do not provide a spatial context for miRNA expression. Whilst in situhybridization (ISH)-based miRNA detection methods with spatial resolution do exist, they often have low sensitivity and signal-to-noise ratios and require extensive optimization for each sample type and probe.
This application note introduces miRNAscope™ – the highly sensitive and specific in situ hybridization assay for detection and visualization of microRNAs, siRNAs, and antisense oligonucleotides (ASOs).
Download the application note to find out more.

DNA copy number variations (CNVs) and gene rearrangements provide vital insights for diagnosis and treatment strategies, especially in cancer. With current methods for profiling these variations lacking tissue and morphological context, Bio-Techne has developed DNAscope™, a novel chromogenic in situ hybridization (ISH) assay for high-resolution spatial detection of DNA aberrations.
This white paper covers the following topics:
• An overview of the current tools for DNA-ISH
• A comparative analysis of DNAscope with currently available DNA-ISH assays
• Using the DNAscope HD Duplex assay for detection of CNVs, translocations, and gene arrangements
Download now to learn more.

Simple Western™ systems, which launched in 2011, have emerged as a powerful tool to replace traditional Western blotting workflows while meeting established guidelines on reproducibility and rigor. In addition to the sensitivity and throughput advantages afforded by complete automation, Simple Western systems create electronic data files that are impervious to manipulation and contain both raw data and analysis that can be easily uploaded to a data repository when it comes time to publish.
This white paper examines how Simple Western overcomes the challenges and pitfalls associated with traditional Western blots to produce publication-ready data that meets the highest standards of data integrity and rigor.

Bio-Techne has compiled a tissue-specific handbook to bring you key publications, protocol guidance, and troubleshooting recommendations for your organoid cell culture.

The ultimate guide to assist you in running your experiments. Download this guide now to learn more about:
Different types of ELISA kits
Which immunoassay is right for you
Detection of molecules using ELISA
ELISA sample preparation
ELISA result calculations
ELISA techniques, tips and best practices

Learn how the simultaneous analysis of multiple factors provides numerous benefits to you and your research:
Collecting data from just 25μl or less of undiluted sample.
Processing samples only once, so multiple data points are derived from a single manipulation.
Generating high volumes of data with minimal sample prep and processing.

This handbook provides a clear and concise introduction to IHC, as well as technical tips, protocols, and a troubleshooting guide to optimize your experiments.
Download the handbook and learn about:
Different variables to consider when performing an IHC experiment
Basic overview of an IHC experiment
Tissue sample preparation and fixation
Controls for IHC
Complementary techniques including RNA in situ hybridization (ISH)
How to optimize IHC workflow
Step-by-step IHC staining protocols and troubleshooting tips

Never strip and reprobe again! Advance your research to perform automated serial immunoassays with the same sample.
Automate the protein separation and immunodetection of traditional western blotting, and eliminate many of the tedious, error-prone steps. With just 3 μL of sample, Jess™ offers pg-level sensitivity on up to 25 samples in 3 hours. Now, Jess comes with RePlex™ so that you can reuse the same 3 μL of sample for automated sequential rounds of probing, all in a fully automated fashion.
In this Application Note, we show how RePlex can be used to detect and quantify pan and phospho isoforms of proteins.
In this Poster, Dr. Dermody demonstrates the application of Replex™ to characterize the P13K/Akt signaling pathway, using sequential analysis of proteins separated and immobilized in a capillary.

Learn more about the role of stress in insulin resistance and dyslipidemia.

This handbook gives you an overview of flow cytometry with numerous data examples, as well as step-by-step detailed protocols and troubleshooting tips to optimize your flow cytometry experiments. In this handbook, you will learn about basic components of a flow cytometer, different types of cytometers, flow cytometry workflow, protocols, and troubleshooting tips.