Table 2 - uploaded by Anand Prakash Maurya
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Source publication
Introduction: Klebsiella pneumonia is an emerging pathogen associated with multidrug resistance both in hospital and community settings. Aminoglycosides, considered to be second line drug for the treatment of such pathogens, become inactive due to acquisition of various resistance determinants by this organism. Objective: The objective of the study...
Context in source publication
Context 1
... susceptibility testing, 94 (53.6%) organisms out of 177, all of which were isolated from urine and pus (Table 1) were found to be resistant against one of the aminoglycosides, (gentamicin, netilimicin, amikacin, kanamycin) ( Table 2) or more antibiotic. All the isolates showed multidrug resistance (MDR) phenotypes. ...
Citations
... The three aminoglycosides studied, namely, Streptomycin, Gentamicin, and Amikacin managed to inhibit 22%, 62% and 63% of the 158 bacterial isolates respectively. This observed high to moderate resistance against these antibiotics is largely due to the bacterial acquisition of aminoglycoside-modifying enzymes or AMEs [14,15]. The β lactams showed some alarming results as 97.5% of all the isolates survived on Ampicillin and 94% survived on Amoxicillin-Clavulanic acid. ...
Objectives
construction of agricultural environmental soil antibiotic resistance profile of the culturable bacteria in Kalyani, India, followed by the screening of ESBL producers harboring blatem, blashv, and blactx-M.
Methods
158 Multi-Drug Resistant (MDR) bacteria were isolated from 18 agricultural sampling points in Kalyani, West Bengal, India followed by their Antibiotic resistance profiling against 15 test antibiotics. They were then screened for the presence of three Extended Spectrum Beta-Lactamase (ESBL) genes, namely blatem, blactx-M and blashv by Double Disk Diffusion test and Polymerase Chain Reaction. The positive candidates were further identified using Amplified Ribosomal DNA Restriction Analysis followed by 16S rDNA sequencing.
Results
lowest resistance was observed against Vancomycin (0%) whereas the highest against the β lactam Ampicillin (97.5%). Very high resistance was observed against the 3rd generation cephalosporin Cefotaxime (90%). 16/158 bacterial isolates were positive for one or more of the three ESBL genes under investigation. 11 out of those 16 isolates (68.75%) harbored at least 2 genes and the rest 31.25% either had the blashv or the blatem gene. Phylogenetic analysis of the prevalent ESBL carrying organisms in the region demonstrated that the genes are being horizontally transferred across different bacterial orders and classes.
Conclusion
Significant antimicrobial resistance noted against 12 out of 15 antibiotics tested. Stenotrophomonas, Myroides, Pseudomonas, Enterobacter, and Serratia are found as culturable MDR bacteria carrying ESBL genes among the isolates. It is the first report of environmental ESBL carriage by Myroides isolated from India.
