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mitochondria morphology and structure in the liver of C. bouderius and P. hypophthalmus were observed by TEM after cold stress (a); RT‐PCR analysis of the expression levels of the genes related to mitochondrial fusion and fission (b, d) and calcium ion transport (e, f) of C. bouderius and P. hypophthalmus.
Source publication
Previous studies have indicated that Cranoglanis bouderius and Pangasianodon hypophthalmus clustered into a sister group. However, there was a significant difference in their minimum tolerated temperatures. To reveal the temperature adaptation-related genes, a genome-wide comparative analysis was performed. First, a chromosome-level draft genome of...
Citations
... In addition, identifying the potential genomic regions under selection is easier when baseline differentiation is low, as expected for phylogenetically closely related taxa [8,9]. In recent years, such genomic-comparing methodology has been increasingly applied to non-model systems and has largely expanded our knowledge of the genetic mechanisms underlying the climate-driven adaptation and speciation in a range of taxa [10][11][12]. However, it is still less clear whether such climate-driven adaptation is also important for genetic differentiation and speciation within marine taxa, such as in teleost fishes. ...
Deciphering the role of climate adaptation in generating genetic divergence and hence speciation is a central question in evolution. Comparisons of genomes of closely related species spanning selective climate gradients are particularly informative in discerning the signatures of selection and thereby providing valuable information concerning the role of climate adaptation in speciation. Here we re-sequenced 99 genomes of the two sister eel-goby species Odontamblyopus lacepedii and O. rebecca, which are endemic to tidal mudflats spanning contrasting latitude gradients, to estimate the influence of divergent climate selection on shaping genome-wide patterns of divergence. The results indicated that genome-wide differentiation between the two species was evident (genome-wide FST = 0.313). Against a background of high baseline genomic divergence, 588 and 1202 elevated divergent loci were detected to be widespread throughout their genomes, as opposed to focused within small islands of genomic regions. These patterns of divergence may arise from divergent climate selection in addition to genetic drift acting through past glacial segregation (1.46 million years ago). We identified several candidate genes that exhibited elevated divergence between the two species, including genes associated with substance metabolism, energy production, and response to environmental cues, all putative candidates closely linked to thermal adaptation expected from the latitude gradient. Interestingly, several candidates related to gamete recognition and time of puberty, and also exhibited elevated divergence, indicating their possible role in pre-zygote isolation and speciation of the two species. Our results would expand our knowledge on the roles of latitude climate adaptation and genetic drift in generating and maintaining biodiversity in marine teleosts.
