iPS cells derived from bone marrow cells. (A) Phasecontrast and (B) GFP fluorescent photomicrographs of iPS cells derived from mouse bone marrow cells showing an embryonic stem cell-like morphology. Bone marrow was isolated from Oct4-GFP mice and reprogrammed using transduction with viral vectors encoding for Oct4, Sox2, Klf4, and c-Myc. Only the colonies expressing endogenous Oct4 (GFP-positive) were expanded to obtain a pure population of iPS cells. (C-F) Fluorescent immunostaining of bone marrow cell-derived iPS cells for expression of Sox2 (red; C). Endogenous Oct4 expression (GFP green; D) continued during in vitro expansion of iPS cells. The nuclei were observed by DAPI staining (blue; E). Figure 3F represents the merged image of AC (original magnifications ¼ 200Â). GFP, green fluorescence protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline .com=ars).

Context in source publication

Context 1

... circumvent the availability issues, the authors have opted for bone marrow- derived stem cells and skeletal muscle-derived myoblasts and successfully reprogrammed the cells into iPS cells using Yamanaka quartet of factors. Using bone marrow-derived stem cells from Oct4-green fluorescence protein þ transgenic mouse, we have successfully generated iPS cells lines that were identified on the basis of their ES cell-like morphology and expression of green fluorescence protein postsuccessful reprogramming (Figs. 2 and 3). Both skeletal myoblasts as well as bone marrow-derived stem cells are readily available from patients in sufficient number and without ethical issues. ...