Figure - uploaded by Pun Yeesin
Content may be subject to copyright.
Source publication
The present study aims to analyze concerned karyotyping and idiograming of the three-spot damselfish (Dascyllus trimaculatus) in Thailand. Chromosomes were prepared from kidney tissues of fish reared at Institute of Marine Science, Burapha University, Chonburi Province. The mitotic chromosomes were harvested by the colchicine-hypotonic-fixation-air...
Similar publications
This study analyzed the karyotype of Lygosoma quadrupes and Scincella melanosticta from northeastern Thailand. Mitotic chromosomes were prepared directly from intestinal epithelial cells of three L. quadrupes and nine S. melanosticta. The chromosomes were stained by conventional staining technique. The karyotype was determined for each species base...
Citations
... There has also been considerable effort in understanding the chromosomes architecture and variation of Dascyllus and other damselfishes. Chromosome number varies between species of Dascyllus as well as within species (Ojima and Kashiwagi 1981;Kashiwagi et al. 2005;Getlekha et al. 2017) giving insight into chromosomal drivers of evolution (Galetti et al. 2000;Hardie and Hebert 2004;Molina and Galetti 2004) and how this variation is manifested ecologically (Molina and Galetti 2004;Martinez et al. 2015). As we shift into the age of genomic natural history where genomic tools offer vastly more detail and statistical power, a reference genome will aid in further refining our understanding of wildlife biology (Hotaling et al. 2021). ...
... The biology, evolution, and biogeography of the three-spot damselfish is relatively well studied using genetic (Bernardi and Crane 1999;Bernardi et al. 2001;McCafferty et al. 2002;Leray et al. 2009Leray et al. , 2010Liggins et al. 2016;Getlekha et al. 2017;Crandall et al. 2019) and genomic tools (Salas et al. 2019(Salas et al. , 2020 and, as we shift further into the age of WGS data and tools, a reference genome is an invaluable resource. Here, we present the chromosomescale genome assembly of a three-spot damselfish, Dascyllus trimaculatus, collected from the Indonesian/Philippine population (Limon et al. in review). ...
... We report sequences for 24 chromosomes of the D. trimaculatus genome with total length and repetitive content (Fig. 2a, Supplementary Fig. 1) that is expected for this species (Arai 1976;Getlekha et al. 2017;Yuan et al. 2018). Interestingly, our Hi-C data also show that chromosomes three and four have strong connections at half the depth of other intra-chromosomal connections (Fig. 2b). ...
Damselfishes (Family: Pomacentridae) are a group of ecologically important, primarily coral reef fishes that include over 400 species. Damselfishes have been used as model organisms to study recruitment (anemonefishes), the effects of ocean acidification (spiny damselfish), population structure, and speciation (Dascyllus). The genus Dascyllus includes a group of small-bodied species, and a complex of relatively larger bodied species, the Dascyllus trimaculatus species complex that is comprised of several species including D. trimaculatus itself. The three-spot damselfish, D. trimaculatus, is a widespread and common coral reef fish species found across the tropical Indo-Pacific. Here, we present the first-genome assembly of this species. This assembly contains 910 Mb, 90% of the bases are in 24 chromosome-scale scaffolds, and the Benchmarking Universal Single-Copy Orthologs score of the assembly is 97.9%. Our findings confirm previous reports of a karyotype of 2n = 47 in D. trimaculatus in which one parent contributes 24 chromosomes and the other 23. We find evidence that this karyotype is the result of a heterozygous Robertsonian fusion. We also find that the D. trimaculatus chromosomes are each homologous with single chromosomes of the closely related clownfish species, Amphiprion percula. This assembly will be a valuable resource in the population genomics and conservation of Damselfishes, and continued studies of the karyotypic diversity in this clade.
... The cell suspensions were then fixed in Carnoy's fixative solution (ratio 3 methanol: 1 acetic acid). The cell suspensions were dropped on the slide (Getlekha et al., 2017;Kasiroek et al., 2017;Supiwong et al., 2017). ...
... In Thailand, including Indochina and the northern Malay peninsula, Alongklod Tanomtong and coworkers have recently focused on fish karyotype analysis and have published 15 reports in Cytologia over the last 2 years on the following fish species: Humpback cardinalfish, Fibramia lateralis (Kasiroek et al. 2017a); Banggai cardinalfish, Pterapogon kauderni (Kasiroek et al. 2017b); triangle butterflyfish, Chaetodon triangulum and yellow butterflyfish, C. andamanensis ); Indian vagabond butterflyfish, Chaetodon decussatus and Lined butterflyfish, C. lineolatus ); Nile tilapia, Oreochromis niloticus (Sriuttha et al. 2017); three-spot damselfish, Dascyllus trimaculatus (Getlekha et al. 2017); ocellated dragonet, Synchiropus ocellatus and picturesque dragonet, S. picturatus (Kasiroek et al. 2017c); crystal eye catfish, Hemibagrus wyckii (Supiwong et al. 2017d); Chevey s sheetfish, Micronema cheveyi (Pinthong et al. 2017); F 1 hybrid catfish: Mekong giant catfish, Pangasianodon gigas striped catfish, P. hypophthalmus and spot pangasius, Pangasius larnaudii Pangasianodon hypophthalmus (Sreeputhorn et al. 2017); whitecheek monocle bream, Scolopsis vosmeri ); snakehead fishes, Channa marulius and C. marulioides (Sarasan et al. 2018); Hihgfin barb fish, Cyclocheilichthys armatus (Chaiyasan et al. 2018); black lancer, Bagrichthys majusculus ; and longfin carp, Labiobarbus leptocheilus . ...
This year marks the 90th anniversary of Cytologia. Modern wheat genetics began 16 years prior to the first issue of Cytologia in 1929. Flaksberger’s wheat stocks, which were sent to Minami in October 1913, were used by Sakamura to determine the number of chromosomes in wheat. Flaksberger’s death in prison in September 1942 was a tragedy for the research community. The Russian and Soviet contribution to modern wheat genetics is immeasurable, but its decline during and after the era of Lysenko was pitiful. This is made clear by the number of publications from each country since the first issue of Cytologia. The publication of Cytologia continued during World War II; after the war, the contribution of India, which became independent at that time, was considerable. Recently, India’s contribution has been relativized, and contributions from other Asian countries including Thailand and Japan, the Middle East and South America have increased, and Cytologia has been supported by increasingly diverse countries.
Chaiyasan P, Mingkwan B, Jantarat S, Suwannapoom C, Cioffi MDB, Liehr T, Talumphai S, Tanomtong A, Supiwong W. 2021. Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system. Biodiversitas 22: 546-554. Karyotype of Java combtail fish, Belontia hasselti, from To Daeng peat swamp forest, Narathiwat Province, southern Thailand, was studied for the first time. Mitotic chromosome preparations were prepared directly from kidney cells from ten male and ten female fish. Conventional staining, NOR banding, and molecular cytogenetics with fluorescence in situ hybridization (FISH) using 5S and 18S rDNAs, as well as microsatellites d(CA)15 and d(CAC)10 as probes were applied. The diploid chromosome number (2n) was 48 and a female heterogametic sex chromosome system (ZZ/ZW) is suggested. The fundamental numbers (NF) were 48 and 49 in males and females, respectively. The karyotype of males comprised 48 telocentric chromosomes while the female ones were composed of one metacentric and 47 telocentric chromosomes. A single Ag-NOR-bearing chromosomal pair was identified. The NOR positions were characterized at the interstitial sub-centromeric region of pair 13, which coincided with signals of 18S rDNA and d(CAC)10 probes. The 5S rDNA signals were located at interstitial sites of the largest telocentric pair. Microsatellite d(CA)15 repeats were highly distributed throughout almost all entire chromosomes except for centromeric regions on some chromosome pairs, including sex chromosomes. The present study is a novel report for a ZZ/ZW sex chromosome system of this fish family in Thailand.
Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The l ocalization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.
The Wada Memorial Award was created in 2011 to encourage the submission of high-quality papers to Cytologia. Coincidentally, the first to fourth holders of the award were experts in karyotype and chromosome analyses among the three regions of the Indomalayan biogeographic realm. Most of Indomalaya was originally covered by tropical and subtropical moist broadleaf forests, with high biodiversity that is currently at risk due to various anthropogenic influences. Karyotype and chromosome analyses provide an essential foundation for maintaining biodiversity through conservation; therefore, in this paper, we focus recent their contributions of the early Wada Memorial Award winners to the three regions of Indomalayan
