Figure 4 - available via license: Creative Commons Attribution 4.0 International
Content may be subject to copyright.
Source publication
Novel therapeutic strategies against difficult-to-treat bacterial infections are desperately needed, and the faster and cheaper way to get them might be by repurposing existing antibiotics. Nanodelivery systems enhance the efficacy of antibiotics by guiding them to their targets, increasing the local concentration at the site of infection. While re...
Contexts in source publication
Context 1
... by uorescence microscopy and confocal laser scanning microscopy (CLSM) showed that gRBP P545 and gCBD SA97 bonded to all intended target strains (Fig. 2d, Supplementary Fig. 1,2). Additionally, both proteins were tested against ve nontarget strains, for which they showed no measurable anity ( Supplementary Fig. 3,4). These results demonstrate an exceptionally broad range specicity within the target strain, while maintaining a narrow range spectrum. ...
Context 2
... pathogen targeting capability of the rifampicin-loaded nanodelivery systems, CLSM assays with DyLight-633-labeled Rif@LUN@RBP P545 and Rif@UPSN@CBD SA97, were performed on DAPI-stained K. pneumoniae and S. aureus. The microscopy images show that ecient binding and cellular uptake of Rif@LUN@RBP P545 occur in K. pneumoniae, but not in S. aureus (Fig. 4a). Similarly, Rif@UPSN@CBD ...
Context 3
... specic binding to S. aureus, but not against K. pneumoniae (Fig. 4a). These results show that Rif@LUN@RBP P545 and Rif@UPSN@CBD SA97 have the intended bacterial targeting ability and specicity, suggesting they may exert ecient site-of-infection pathogen targeting ...
Context 4
... SA97 in vitro, timegated uorescence imaging was performed on lung tissue of infected mice, after administering the delivery systems. The results showed that Rif@LUN@RBP P545 was signicantly accumulated in the lungs of K. pneumoniae-infected mice, while no trend of Rif@LUN@RBP P545 accumulation was observed in the lungs of non-infected mice (Fig. 4b). Similarly, Rif@UPSN@CBD SA97 was signicantly accumulated in the lungs of S. aureus-infected mice, while no trend of Rif@UPSN@CBD SA97 accumulation was observed in the lungs of non-infected mice (Fig. 4c). These results demonstrate that RBP P545 and CBD SA97 -guided nanodelivery systems have comparable or even better bacterial ...
Context 5
... in the lungs of K. pneumoniae-infected mice, while no trend of Rif@LUN@RBP P545 accumulation was observed in the lungs of non-infected mice (Fig. 4b). Similarly, Rif@UPSN@CBD SA97 was signicantly accumulated in the lungs of S. aureus-infected mice, while no trend of Rif@UPSN@CBD SA97 accumulation was observed in the lungs of non-infected mice (Fig. 4c). These results demonstrate that RBP P545 and CBD SA97 -guided nanodelivery systems have comparable or even better bacterial targeting ability than the already impressive CARG peptide-guided nanodelivery system 21 . Additionally, it suggests that RBP P545 -guided Rif@LUN@RBP P545 and CBD SA97 -guided Rif@UPSN@CBD SA97 may show enhanced ...
