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and biochemical characteristics of nonsmoking and smoking men and women before dietary supplementation with aged garlic

and biochemical characteristics of nonsmoking and smoking men and women before dietary supplementation with aged garlic

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and biochemical characteristics of nonsmoking and smoking men and women...
Human Nutrition and Metabolism Research Communication Dietary Supplementation with Aged Garlic Extract Reduces Plasma and Urine Concentrations of 8Iso Prostaglandin F2 in Smoking and Nonsmoking Men and Women1
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F2-isoprostanes are recently described prosta- glandin F isomers produced by cyclooxygenase-independent free radical peroxidation of arachidonic acid. Their quantifi- cation in plasma and urine is a sensitive and specific indicator of lipid peroxidation and, hence, of oxidative stress in vivo. Some components of garlic are known to possess antioxid...
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... clinical and biochemical characteristics of the smoking and nonsmoking groups at the beginning of the trial are given in Table 1. All subjects were normolipidemic and had normal kidney function. ...

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Determination of 8-iso-PGF2? as Oxidative Stress Marker in Human Urine by High Performance Liquid Chromatography with Tandem Mass Spectrometry
Article
  • Feb 2010
This study aimed to develop analytical method for 8-isoprostanes as biomarkers for oxidative stress with LC/MS/MS technique and to apply the method for human urine samples. Analyzed compounds for urinary oxidative stress markers were 7 stereo-isomers of prostaglandins and the internal standard (iso-) was used to adjust the recovery rate. The method for determining urinary iso- consisted of solid phase extraction and LC/MS/MS detection. Separation of isomers of prostaglandins completed by porous graphitic carbon column and buffer solution. Detection limits for urinary markers of oxidative stress, iso- with LC/MS/MS were 0.01 ng/ml by S/N ratio 3 and 0.028 ng/ml by calculated as to FDA method. The recovery (92.8~101.9%) and precision (8.8~20.7%) of analysis were feasible for detecting iso- in real human urine samples. We detected 4 isomers of prostaglandins in human urine samples. Mean (standard deviation) of urinary iso- concentration were 0.231 (0.117) ng/mg creatinine for smoking group and 0.154 (0.082) ng/mg creatinine for non-smoking group.
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