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a − c. Rhodotorula aurantiaca . d − f. R. graminis . g − i. R. marina . j − l. R. minuta . m − o. R. mucilaginosa . p − r. R.slooffiae . Bars = 10 μ m for b, e, h, k, n, & q (photographs by light microscope); Bars = 1 mm for c, f, I, l, o, & r (photographs by SEM).
Source publication
Characteristics of six red yeasts isolated from seawater, Rhodotorula aurantiaca, R. graminis, R. marina, R. minuta, R. mucilaginosa, and R. slooffiae are described. All except R. mucilaginosa are new records to Tai-wan.
Contexts in source publication
Context 1
... Growth on YM agar: After 7 days at 25°C, the streak culture is redishorange, smooth, glossy and convex. The edge is entire (Fig. ...
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... in YM broth: After 3 days at 25°C, cells are subglobose or ovoidal. Cell size are 3.0−5.0 × 3.0−6.5 µm, and occur clusters (Fig. 1b & 1c). Reproduction by multilateral ...
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... Growth on YM agar: After 7 days at 25°C, the streak culture is orange, smooth, glistening and convex. The edge is entire (Fig. ...
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... in YM broth: After 3 days at 25°C, cells are globose to ovoidal or elongate, 2−4 × 3−11.5 µm (Fig. 1e & 1f (Fig. ...
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... in YM broth: After 3 days at 25°C, cells are globose to ovoidal or elongate, 2−4 × 3−11.5 µm (Fig. 1e & 1f (Fig. ...
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... in YM broth: After 3 days at 25°C, cells are ovoidal to globose, 2.0−4.0 × 3.5−8.0 µm (Fig. 1h & 1i) Morphology. Growth on YM agar: After 7 days at 25°C, the streak culture is pink, smooth, glossy but not mucoid, and low convex. The edge is entire (Fig. ...
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... in YM broth: After 3 days at 25°C, cells are ovoidal to globose, 2.0−4.0 × 3.5−8.0 µm (Fig. 1h & 1i) Morphology. Growth on YM agar: After 7 days at 25°C, the streak culture is pink, smooth, glossy but not mucoid, and low convex. The edge is entire (Fig. ...
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... in YM broth: After 3 days at 25°C, the cells are ovoidal, globose or elongate, 4.0− 5.0 × 5.5−7.0 µm (Fig. 1k & 1l), single or in pair. Reproduction by multilateral ...
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... 1928. Trans. R. Soc. Can., Sect. V, 22: 187−225. Morphology. Growth on YM agar: After 7 days at 25°C, the streak culture is salmonpink to orange, smooth, glistening and low convex. The margin is entire (Fig. ...
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... in YM broth: After 3 days at 25°C, the cells are ovoid, globose or spherical measuring 4.5−6.8 × 4.0−8.0 µm (Fig. 1n & 1o); reproduction is by multilateral budding and cells may occur singly, in pairs, in short chains or small ...
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... Growth on YM agar: After 7 days at 25°C, the streak culture is pink, smooth, glossy but not mucoid, the cross section id flat to low convex. The edge is entire (Fig. ...
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Citations
... The cell size of both also has the same size. The difference between them lies in the colony profile, R. taiwanensis has a mountainous profile, while R. mucilaginosa is flat (Chang and Wang 2002). ...
Aspergillus flavus is widely known as an aflatoxin-producing fungus that frequently contaminates feed and affects livestock, which leads to severe health problem for animal and human. Biological agents have been proven to prevent this contamination since they can produce metabolites which have antagonistic activity. In this study, phylloplane yeasts isolated from Moringa oleifera leaf have shown an ability to inhibit the growth of Aspergillus flavus UNJCC F-30 collected from chicken feed. This research was conducted in three stages: (1) yeast isolation (leaf washing and direct method), followed by (2) antagonistic test using dual culture method, and (3) molecular identification using D1/D2 region of 26S rDNA. In the first stage, 38 yeast isolates have been succesfully obtained. These isolates were of different colors: peach pigment (60.5%), the non-pigmented yeast (26.5%), cream (10%), and orange (3%). Antagonistic activity against A. flavus UNJCC F-30 was tested based on growth, sporulation, and the presence of clear zones. Screening result showed that 12 yeast isolates are capable of inhibiting A. flavus UNJCC F-30. Among them, 4 isolates with the code K4, K10, K15, and K26 showed the highest antagonist ability. Molecular identification resulted that the 4 isolates show a similar identity with Aureobasidium pullulans UWFP 993 (100%), Aureobasidium melanogenum QCC:M017/17 (99%), Aureobasidium melanogenum QCC:M017/17 (100%) and Rhodotorula taiwanensis CBS:11729 (99%), respectively. Isolate K10 exhibited the highest percentage of inhibition activity among all isolates which is potential for application as biocontrol agent against A. flavus. As A. pullulans is a common yeast found on leaf surfaces of many Indonesian flora, therefore it can be considered as safe and alternative to reduce fungal contamination from A. flavus in feed chicken.
Aseptic packaging of high quality beverage is necessary and its cold-pasteurization or sterilization is vital. Studies on application of ultrafiltration or microfiltration membrane to cold- pasteurization or sterilization for the aseptic packaging of beverages have been reviewed. Designing and manufacturing ultrafiltration or microfiltration membrane systems for cold-pasteurization or sterilization of beverage are based on the understanding of size of microorganisms and theoretical achievement of filtration. It is concluded that adaptability of membrane filtration, especially its combination with other safe cold method, to cold- pasteurization and sterilization for the aseptic packaging of beverages should be assured without a shadow of doubt in future.
Karakterisasi morfologi khamir penting dilakukan sebagai pengenalan awal suatu isolat khamir. Sedangkan, pengamatan terhadap pemanfaatan sumber karbon digunakan untuk mengetahui sumber karbon yang dapat digunakankan oleh khamir untuk perbanyakan dan peningkatan kemampuannya sebagai agens biokontrol. Penelitian ini bertujuan untuk mengkarakterisasi morfologi dan mengetahui pemanfaatan sumber karbon oleh khamir antagonis patogen antraknosa pada cabai (Colletotrichum acutatum). Karakterisasi morfologi khamir dilakukan dengan mengamati secara makroskopis koloni enam spesies khamir dalam medium PDA. Karakterisasi morfologi juga dilakukan dengan mengamati mikroskopis sel khamir di bawah mikroskop cahaya photomicrograph multi eyepiece (Zeiss Axiocam). Pengamatan pemanfaatan sumber karbon oleh khamir dilakukan dengan menggunakan BIOLOGTM (MicrologTM System, Release 5.2). Karakteristik morfologi enam spesies khamir antagonis menunjukkan bahwa koloni khamir didominasi warna krem keputihan dengan permukaan kasar, kusam, dan tepi koloni tidak rata. Karakteristik sel secara mikroskopis menunjukkan bentuk sel didominasi oleh bentuk silinder, dengan ukuran bervariasi. Sebagian besar khamir bersifat dimorfik. Pengamatan pemanfaatan sumber karbon oleh khamir antagonis menunjukkan Aureobasidium pullulans, Pseudozyma hubeiensis, Pseudozyma aphidis dan Pseudozyma shanxiensis dapat memanfaatkan 20 sumber karbon yang sama yaitu Tween 80, Arbutin, D-Gluconic Acid, D-Glucuronic Acid, D-Ribose, Salicin, ɤ-AminoButyric Acid, Bromosuccinic Acid, Fumaric Acid, β-Hydroxy Butyric Acid, L-Lactic Acid, LMalic Acid, Succinamic Acid, Succinic Acid, Alaninamide, L-Alanine, L-Aspartic Acid, LGlutamic Acid, Putrecine, dan Quinic Acid. Sedangkan, dua spesies khamir yaitu Rhodotorula minuta dan Candida tropicalis dapat memanfaatkan dua sumber karbon yang sama yaitu D-Trehalose dan D-Galactose plus D-Xylose.
