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(a) Total 0.25-N-HCl-soluble proteins from unincubated nuclei; positions of histones HI and HlO are marked. (b) Acid-urea gel electrophoresis of 5%-perchloric-acid-soluble proteins from chromatin resistant to solubilization by digestion of nuclei with micrococcal nuclease following incubation with I mM NAD c o n t a i n i n g 32p-NAD as tracer. (c) Autoradiograph of b. 

(a) Total 0.25-N-HCl-soluble proteins from unincubated nuclei; positions of histones HI and HlO are marked. (b) Acid-urea gel electrophoresis of 5%-perchloric-acid-soluble proteins from chromatin resistant to solubilization by digestion of nuclei with micrococcal nuclease following incubation with I mM NAD c o n t a i n i n g 32p-NAD as tracer. (c) Autoradiograph of b. 

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Fig. 3. Electrophoretic sizing of DNA contained in chromatin obtained...
Fig. 3 is a photograph of the DNA contained in chromatin prepared as in...
Fig. 5. Electrophoretic sizing of DNA contained in chromatin obtained...
Fig. 6. Electrophoresis in an acid-urea gel of the...
Fig. 7. (a) Total 0.25-N-HCl-soluble proteins from unincubated nuclei;...
Increased thermal stability of solubilized chromatin after poly(ADP-ribose) synthesis
Article
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  • Oct 1983
A hyperthermic shift in the hyperchromicity curve of thermally denatured swine aortic-smooth-muscle-cell chromatin solubilized by digestion of nuclei with micrococcal nuclease was observed after the chromatin was incubated under conditions to allow poly-(ADP-ribose) synthesis by the endogenous poly(ADP-ribose) polymerase. When the order of solubili...
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Context 1
... of these proteins in the chromatin resistant to solubilization by micrococcal nuclease digestion was performed including [32p]NAD as tracer in the incubation. From the stained gel shown in Fig. 7, lane b, it is apparent that the H1 ~ band is the major species ...
Context 2
... (Fig. 7, lane c) of the gel revealed that the H1 has been poly ADP-ribosylated to varying degrees, and, consequently, was diffusely spread throughout the upper region of the gel due to retardation of its mobility by the attached ...

Citations

Benzamide-inhibitable alterations in spectral properties of chromatin accompany DNA repair in UVC-exposed L5178Y cells
Article
  • Feb 1988
  • RADIAT ENVIRON BIOPH
We examined the response of chromatin to increasing NaCl and MgCl2 concentrations in UVC-irradiated L5178Y (LY) R and S cells, using the spectral index method (Dixon and Burkholder 1985). We have found an alteration in chromatin properties 1 h after UVC-irradiation of repair proficient LY-S cells, but no change in repair deficient LY-R cells. The change was shown as lowered spectral index, indicating that at given Na+ and Mg++ concentrations (1 or 200 mM NaCl, 0 or 0.5 mM MgCl2) chromatin from UVC-irradiated LY-S cells was more compact than that from unirradiated ones. Benzamide treatment reversed the effect of UVC-irradiation in LY-S cells and did not change the response pattern of chromatin from LY-R cells or unirradiated LY-S cells.
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