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The aim of this work was to investigate the influence of an anti-inflammatory agent, the bulky counterion named glucosamine (Gl +) , in sodium dodecylsulphate (SDS) in 2 ways: 1) by titration of SDS solutions with different concentrations of Gl + ; and 2) by titration of Gl + with SDS solution with concentration close to the critical micellar conce...
Contexts in source publication
Context 1
... they have been used as a model of membranes to simulate binding studies of proteins, peptides, and drugs due to some similarities with anionic biological membranes. 3,11−15 Glucosamine hydrochloride (Gl + ) (Figure 1) is an anti-inflammatory agent having an excellent tox- icity profile, 16−18 and is an active drug needed to form collagen, whose oral ingestion causes reduction of osteoarthritis. 16,18−21 Its mode of action is reported to occur in the intracellular environment. ...
Context 2
... order to evaluate the spatial topology of the SDS/Gl + assembly, NOESY experiments of the SDS(15 mM)/Gl + (5 mM) system in D 2 O at 25 • C were conducted. Figure 10 shows the contour map of NOESY, where the cross peaks indicate the proximity between nuclei within the limit of 5 ˚ A in the space, due to electromagnetic dipolar coupling. 37−40 Cross peaks can be observed between SDS-H1 hydrogens and H6-and H11-Gl + hydrogens, indicating that the protons are spatially close. ...
Citations
... Exercise-induced knee injury mainly refers to the injuries of knee joint tissue structures or functions that may occur in any sports event, with manifestations of pain, swelling, restricted knee movement, and symptoms such as redness, swelling, and hot pain in the acute stage. A cold compress can stop the bleeding, reduce swelling, and relieve pain by promoting vasoconstriction, reducing local congestion, and lowering tissue temperature [17][18][19][20]. Clinical treatment of exercise-induced knee injuries is classified into nondrug treatment and drug treatment. ...
... Nondrug therapy mainly employs comprehensive rehabilitation therapies such as massage, acupuncture, and Chinese medicine herbal application to mitigate knee joint pain and promote the recovery of knee joint function. Nonsteroidal antiinflammatory drugs, analgesics, intraarticular injections (such as sodium hyaluronate and glucocorticoids), and chronically acting drugs that relieve symptoms (such as glucosamine hydrochloride) are mostly used in drug therapy [20][21][22][23]. Glucosamine hydrochloride is an amino monosaccharide obtained from chitin by hydrochloric acid hydrolysis. ...
Objective:
To assess the application value of comprehensive rehabilitation therapy plus glucosamine hydrochloride for exercise-induced knee injuries and its effect on knee function.
Methods:
A total of 96 patients with an exercise-induced knee injury who were admitted to our hospital from February 2019 to February 202 were recruited and assigned at a ratio of 1 : 1 with matched general information to a control group (n = 45) or an experimental group (n = 51). Both groups of patients received comprehensive rehabilitation therapy, and the patients in the experimental group were daily given additional glucosamine hydrochloride tablets for 8 weeks.
Results:
The experimental group showed a higher treatment efficacy than the control group (P < 0.001). After the treatment, the VAS scores and C-reactive protein of the two groups showed a decline, with a lower result in the experimental group than in the control group (P < 0.001). The Lysholm knee scores were increased in the two groups after the treatment, and the experimental group had a higher score (P < 0.001). After the treatment, patients of both groups showed reduced five-times-sit-to-stand-test (FTSST) results, with a better outcome obtained in the experimental group (P < 0.001).
Conclusion:
Comprehensive rehabilitation therapy plus glucosamine hydrochloride effectively improves the clinical efficacy of exercise-induced knee joint injuries and enhances the knee joint rehabilitation of the patients.
... CPC (see Fig. S2 in supplementary material) is a cationic surfactant used as a mouthwash for more than 70 years, showing biocidal activity against a broad spectrum of oral cavity bacteria. Cationic drugs commonly interact with anionic phospholipids, inducing damage on membrane structure resulting in loss of cellular components, inactivation of ion channels, inhibition of cell growth and consequent cell death [22][23][24]. However, the investigation of CPC/βCD inclusion compounds against microorganisms is still a task that must be more investigated, especially if the βCD is able to change the interaction mechanism with bacterials cells. ...
Cetylpyridinium chloride (CPC) is a cationic surfactant, which has a biocidal activity against a broad spectrum of bacteria, including Staphylococcus aureus. This microorganism, although frequently found in the normal human microbiota, can become a pathogen that causes a wide variety of infections. Thus, the present work aims to investigate the effect of the β-cyclodextrin (βCD) on CPC antimicrobial activity against S. aureus, especially in the mechanism of interaction with S. aureus membrane. For these purposes, in vitro antimicrobial susceptibility of CPC and CPC/βCD compounds against S. aureus were determined by calculating the lower concentration capable of reducing cell viability by 50 and 100 percent, and the kinetics of bacterial death were evaluated by kill curves for the two systems. After that, the colloidal mechanisms of interaction of the CPC and CPC/βCD against S. aureus were investigated by Dynamic Light Scattering (DLS) and Zeta Potential (ZP) titrations. Finally, the thermodynamic parameters of drug-cell interaction were determined by Isothermal Titration Calorimetry (ITC), in order to obtain deeper understanding of the mechanism of drug interactions. The results of DLS, ZP and ITC showed that in presence of βCD occurs a different mechanism of interaction with S. aureus membrane, explaining therefore the higher antimicrobial activity of CPC/βCD system.
... Similar results have been found for interaction of other cationic surfactants with bCD. For example, Petek et al [38] showed that bCD increases the cmc of C 12 , C 14 and C 16 alkyltrimethylammonium bromides with a rate of 0.69, 0.63 and 0.65 Thermodynamic parameters of 10.0 mM titrated bCD interaction in 0.5 mM CPC. respectively. Additionally, rates with the same magnitude were found by Jobe et al [18] for anionic SDS surfactant in presence of several cyclodextrins: 0.55 for aCD, 0.70 for bCD, 1.04 for gCD, and 0.80 for HPbCD. ...
In the present work we have performed a structural and thermodynamic characterization of supramolecular structures formed by interaction between cethylpyridine chloride (CPC) surfactant and β-cyclodextrin (βCD), using several physical-chemistry methods. Initially, qualitative CPC/βCD interactions in solid state were confirmed by FTIR and TGA/DTA. 2D NMR ROESY experiment showed strong correlations between hydrogens of βCD cavity with aromatic and aliphatic hydrogens of CPC, suggesting the existence of different complexes in solution. This information was corroborated by structures assessed by computational approach and stoichiometric coefficient obtained by ITC (N = 1.45). ITC experiments (at constant concentration of CPC) also showed that host-guest complexation occur with very high binding constant (K b = 38,300.0), and driven by enthalpy and entropy. However, as CPC is an amphiphilic molecule, it is able to form micelles at critical micellar concentration close to 1 mM. In presence of βCD, it was observed that micellization was delayed, so that the cmc values increased according to the empiric equation: cmc = 1.02 + 0.5[βCD]. Moreover, thermodynamic data obtained by combination of conductometric and isothermal calorimetry titrations showed that the presence of increasing concentrations of βCD causes an increase of free energy of micellization, specially by gradual reduction of entropy of micellization, due to the difficult of micelles encapsulate the inclusion compounds. The overall study was rationalized in terms of competition between micellization vs. host-guest complexation.
