Fig 1 - available via license: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Content may be subject to copyright.
(a) Plant bioelectric potential measurement system, (b) Typical bioelectric potential response to light irradiation and definition of evaluation parameter, Von.
Source publication
Plant bioelectric potential response changes corresponding to the plant physiological activities. The activities and the growth of plant are influenced by the light irradiation conditions. In this study, we aim to obtain the relationship between the light irradiation conditions and the plant activity using plant bioelectric potential. We compared t...
Contexts in source publication
Context 1
... 1 (a) shows the measurement system of the plant bioelectric potential. The bioelectric potential difference between the two electroencephalographic (EEG) needle type electrodes (the diameter: 0.22 mm) was measured with a high-input-impedance (> 1G ohm) digital multimeter (DMM in Fig. 1). The plant was placed in a 22.4 L closed vessel, and the change in CO2 concentration caused by plant metabolism such as photosynthesis and respiration was measured using an NDIR-type CO2 analyzer. The light source consisted of blue (470 nm), green (525nm), and red (660 nm) LEDs, and light irradiation patterns were controlled by a ...
Context 2
... and stopped every 30 minutes were measured and recorded by a computer at a sampling interval of 1 second. An ambient temperature and the light intensity were fixed on 25 o C and 35 or 60 mol/m 2 s, because the plant physiological activities and the bioelectric potential responses are influenced by an ambient temperature and light intensity [4]. Fig. 1 (b) shows the typical bioelectric potential response when illumination was started. We defined the difference Von as that between the biggest potential increase and the value before light irradiation. The sample plants were some strawberries grown in hydroponics after 3 months from seeding. (a) ...
Similar publications
Arbuscular mycorrhizal fungi (AMF) colonize the roots of most terrestrial plant species, improving plant growth, nutrient uptake and biotic/abiotic stress resistance and tolerance. Similarly, plant growth promoting bacteria (PGPB) enhance plant fitness and production. In this study, three different AMF (Funneliformis mosseae, Septoglomus viscosum,...
Citations
... These nano-sensors are capable of probing bioelectric potential changes in plants. This allows for on-line monitoring of temperature, light intensity, and humidity in various plant species [101,104]. For instance, Strano group demonstrated an outstanding sensitivity of SWNT for detection of stress-induced hydrogen peroxide (H 2 O 2 ) signaling waves in seven different plant species [102]. ...
Our civilization has to enhance food production to feed world’s expected population of 9.7 billion by 2050. These food demands can be met by implementation of innovative technologies in agriculture. This transformative agricultural concept, also known as digital farming, aims to maximize the crop yield without an increase in the field footprint while simultaneously minimizing environmental impact of farming. There is a growing body of evidence that Raman spectroscopy, a non-invasive, non-destructive, and laser-based analytical approach, can be used to: (i) detect plant diseases, (ii) abiotic stresses, and (iii) enable label-free phenotyping and digital selection of plants in breeding programs. In this review, we critically discuss the most recent reports on the use of Raman spectroscopy for confirmatory identification of plant species and their varieties, as well as Raman-based analysis of the nutrition value of seeds. We show that high selectivity and specificity of Raman makes this technique ideal for optical surveillance of fields, which can be used to improve agriculture around the world. We also discuss potential advances in synergetic use of RS and already established imaging and molecular techniques. This combinatorial approach can be used to reduce associated time and cost, as well as enhance the accuracy of diagnostics of biotic and abiotic stresses.
... An emerging class of sensor technologies that can be conveniently applied to monitor plant growth and dynamics is wearable sensors. The measurement of bioelectric potential changes in plants can be used to detect changes in environmental factors such as atmospheric pressure, temperature, humidity and light intensity 82 . Ochiai et al. compared commercially available boron-doped diamond (BDD), silver and platinum plate electrodes for measuring electrochemical signals in potted Opuntia hybrid plants and also in three different ground-planted trees 83 . ...
Innovative approaches are urgently required to alleviate the growing pressure on agriculture to meet the rising demand for food. A key challenge for plant biology is to bridge the notable knowledge gap between our detailed understanding of model plants grown under laboratory conditions and the agriculturally important crops cultivated in fields or production facilities. This Perspective highlights the recent development of new analytical tools that are rapid and non-destructive and provide tissue-, cell- or organelle-specific information on living plants in real time, with the potential to extend across multiple species in field applications. We evaluate the utility of engineered plant nanosensors and portable Raman spectroscopy to detect biotic and abiotic stresses, monitor plant hormonal signalling as well as characterize the soil, phytobiome and crop health in a non- or minimally invasive manner. We propose leveraging these tools to bridge the aforementioned fundamental gap with new synthesis and integration of expertise from plant biology, engineering and data science. Lastly, we assess the economic potential and discuss implementation strategies that will ensure the acceptance and successful integration of these modern tools in future farming practices in traditional as well as urban agriculture.
... Light stimulation is also adequate, but unlike thermal, the change in leaf illumination does not produce potential for action and is not a reflection of electrical processes in chloroplasts under photosynthesis (Szechyńska-Hebda & Karpiński, 2013;Hasegawa et al., 2016). The stimulation during experiments is quite easy to ask and control its parameters with the help of a luxmetre. ...
... This component is related to the activity of the proton H + pump. The total flow of H + ions from the intercellular medium to the middle of the cell may be due to the depolarization of the cell caused by the action of light (Pjatygin et al., 2006;Hasegawa et al., 2016;Lyu & Lazár, 2017). The results of experiments, however, do not exclude the possibility of absorption of light by chloroplasts, which leads to the activation of zones in plasmolma ion channels penetrating to protons, and can cause a change in pH on the cell surface (Vodeneev et al., 2012). ...
Plant biopotentials can be used to evaluate their functional state and mechanisms for adaptation to changes in external conditions of their cultivation. The paper is devoted to the experimental study of the dynamics of total potential of maize leaves caused by cold and heat stimuli on the background of photopotential during continuous light stimulation. In the experiments, a specially designed stimulator was used that allowed simultaneous exposure of the plant to light and to thermal irritation. Studies have shown that background continuous light stimulation with white light with a brightness of 250 lux results in an increase in the amplitude of total action potentials caused by rhythmic cold stimulation. The amplitudes of "cold" potentials grew synchronously with the growth of the potential of hyperpolarization under the influence of photostimulation. With the termination of light stimulation, the amplitude of "cold" potentials stabilized. It is assumed that this effect is due to an increase in the amplitude of potentials of action, which correspond to the total potential due to the hyperpolarization of the membranes of the cells that generate them. Such hyperpolarization is due to an increase in the active transport of H+ ions through the membrane of cells in the light phase of photosynthesis. It has been shown that during pulsed heat stimulation, the preliminary continuous background light stimulation results in a decrease in the amplitude of "heat" potentials, a reduction in their duration, and the appearance of a short latent hyperpolarization potential in their initial phase. It is established that these changes correlate with the growth of the potential of hyperpolarization caused by background light stimulation. Based on the analysis of the detected changes, it was deduced that an increase in the level of hyperpolarization increases the threshold of excitability of cell membranes generating these potentials. When the photostimulation was switched off, the level of hyperpolarization decreased, but the amplitudes of the "heat" potentials increased. At the same time, the duration of the potentials increased sharply, and the elements characteristic of the variable potentials appeared in them. This may indicate a significant increase in sensitivity to heat irritation with a decrease in the level of hyperpolarization.
The possibility of using electrochemical impedance spectroscopy (EIS) for direct and real-time monitoring of plants was investigated. Since EIS is an in situ monitoring technique and the obtained signals tend to reflect the ions in plant cells and tissues, it can be used to observe the ion fluctuations that result from the changes in the lighting conditions. Changes in EIS signals and the fitted parameters were observed upon application of an external stimulation to a Marchantia polymorpha individual, which has been known to cause the movement of calcium ions in cellular tissues. In addition, the EIS signals and fitted parameters also changed by altering the lighting conditions. Although further investigation is required, these fundamental experiments indicate that EIS could be applied to monitor in situ ionic phenomena that occur in plants.
Application of electrochemical impedance spectroscopy (EIS) analytical theory for characterizing cellular tissues and cell membrane activities was investigated. Direct observation of ions in the plant cells was the main focus of the research. A new equivalent circuit is presented for cellular tissues; it involves a non-uniform “capacitance-like element,” called ‘constant phase element’. The proposed circuit model is based on an accurate understanding of the in situ plant observation using EIS measurements. Additionally, the growing conditions of the plant were experimentally monitored using EIS. We noted that the time dependence of our circuit model could be used to evaluate the non-uniformity of the interfacial polarization of the cell membrane through EIS, using non-destructive surface-contact electrodes. Although further investigations are still required, the results indicate that the proposed equivalent circuit may reveal the dynamics of plants and be useful for non-destructive plant monitoring.
