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( a ) Magnetic tweezers and fl uorescence microscopy system used in our lab. (1) Ferromagnetic core tip. (2) Two coiled copper wires. (3) Oil objective (63×). (4) Micromanipulator . ( b ) Schematic representation of the magnetic tweezers and imaging setup. The computer-controlled current generator is connected to a power amplifi er (not depicted)
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The aim of this chapter is to present an innovative technique to visualize changes of the f-actin cytoskeleton in response to locally applied force. We developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated wit...
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