Fig 1 - uploaded by Tawanda Elias Maguvu
Content may be subject to copyright.
(a) Growth evaluation of Serratia marcescens 39_H1 at 25 C; (b) Phosphate solubilisation assay of Serratia marcescens 39_H1.
Source publication
Here, we report a high quality annotated draft genome of Serratia marcescens 39_H1, a Gram-negative facul-tative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate inoculum during anaerobic digestion of water hyacint...
Contexts in source publication
Context 1
... strain 39_H1 was selected based on its distinct characteristics. The bacterial strain showed the production of the pigment, prodigiosin, when cultivated at 25 C (Fig. 1a) and this initially identified it as Serratia sp. Based on the partial sequence analysis of the 16S rRNA genes, the bacterial strain was identified to be 99 % homologous to Serratia marcescens and was deposited at the Genbank under the accession number ...
Context 2
... strain also showed zone of clearance after 7 days of incubation on Pikovskaya's agar which signifies its ability to solubilise phosphate (Fig.1b). Quantification of the strain's ability to solubilise phosphate showed an SI of 3.1. ...
Context 3
... dehydratase (G4234_18415). 2-keto-3-deoxy-6-phosphogluconate was then oxidised to pyruvate and glyceraldehyde-3-phosphate by 2-keto-3-deoxy-6-phosphogluconate aldolase (G4234_10110). In the course of the phosphate solubilising assay, the strain possibly secreted an organic acid that initiated the formation of clearance zone around it (Fig. 1b), suggesting its ability as phosphate solubiliser and an acidogenic bacterial ...
Similar publications
A lack of the complete pig proteome has left a gap in our knowledge of the pig genome and has restricted the feasibility of using pigs as a biomedical model. We developed the tissue-based proteome map using 34 major normal pig tissues. A total of 5841 unknown protein isoforms were identified and systematically characterized, including 2225 novel pr...
Citations
... CP-13 decreases Cd uptake and concomitant lipid peroxidation in maize cultivars, showing its high potential in terms of plant growth augmentation and Cd remediation plans [249]. Serratia marcescens AHPC29 can be considered as a new agent for the management of Bursaphelenchus xylophilus, which is a destructive and invasive pathogen in forestry [250], with Obi et al. [251] reporting that Serratia marcescens 39-H1 was able to increase the hydrolysis of lignocellulosic biomass, being a plant-growthpromoting organism. Bhatta et al. [252] reported that Serratia marcescens DB1 is a plantgrowth-promoting rhizobacterium with an innate ability to resist heavy metals such as Cr, Ni, and As, which can stimulate the bioavailability of essential elements for plant uptake and keep the balance of Na + /K + ions in rice shoots. ...
The application of biostimulants has been proven to be an advantageous tool and an appropriate form of management towards the effective use of natural resources, food security, and the beneficial effects on plant growth and yield. Plant-growth-promoting rhizobacteria (PGPR) are microbes connected with plant roots that can increase plant growth by different methods such as producing plant hormones and molecules to improve plant growth or providing increased mineral nutrition. They can colonize all ecological niches of roots to all stages of crop development, and they can affect plant growth and development directly by modulating plant hormone levels and enhancing nutrient acquisition such as of potassium, phosphorus, nitrogen, and essential minerals, or indirectly via reducing the inhibitory impacts of different pathogens in the forms of biocontrol parameters. Many plant-associated species such as Pseudomonas, Acinetobacter, Streptomyces, Serratia, Ar-throbacter, and Rhodococcus can increase plant growth by improving plant disease resistance, synthesizing growth-stimulating plant hormones, and suppressing pathogenic microorganisms. The application of biostimulants is both an environmentally friendly practice and a promising method that can enhance the sustainability of horticultural and agricultural production systems as well as promote the quantity and quality of foods. They can also reduce the global dependence on hazardous agricultural chemicals. Plant growth promoting rhizobactera, and Stenotrophomonas. The aim of this manuscript is to survey the effects of plant-growth-promoting rhizobacteria by presenting case studies and successful paradigms in various agricultural and horticultural crops.
... Bacteria were isolated from 1 ml of digestate samples according to the method reported in Mukhuba et al. [34]. Characterization of bacteria for hydrolytic abilities was conducted by spot plating 5 µl of nutrient broth overnight grown bacterial cultures on sterile carboxymethyl cellulose (CMC) agar as detailed in Obi et al. [35]. ...
... The prokaryotic community of the anaerobic digesters is of immense benefit to the AD process and this is attributed to their ability to survive in sensitive environments like anaerobic digesters and participate in various phases of AD. Treatment 11H produced the highest cumulative methane as opposed to the consortium treatment that generated the least methane, possibly due to the enhanced hydrolytic rate of the inoculated bacteria [35]. Further exploration of the bioaugmentation agent that enhanced the metabolism of the substrates in treatment 11H exhibited the potential of the bioaugmentation agent to enhance the metabolic process of AD of different substrates for subsequent methane production. ...
... before and after bioaugmentation is a reflection of its survival abilities in such a complex and sensitive environment. The facultative anaerobes, though not resilient, potentially improved hydrolysis of substrates and subsequent increase in methane production in the majority of the treatments [35]. Inoculated microbes including Serratia did not thrive during the batch culture assay as they were not recorded as the predominant bacteria (>1%) after the bioaugmentation process. ...
Optimization of anaerobic digestion (AD) of lignocellulosic substrates via bioaugmentation for enhanced biomethane production is imperative. Here, eight different screened bacterial isolates were used to augment different batch anaerobic digesters that comprised freshly chopped water hyacinth (WH) and cow dung (CD), at 2% total solids (w/v). Methane production was monitored at intervals and DNA metabarcoding of the 16S rRNA genes revealed the dynamics of the prokaryotic community structure of the anaerobic digesters. Obtained results suggest bioaugmentation improved the abundance and diversity of the prokaryotic community. The treatment that was inoculated with Serratia marcescens produced the highest cumulative methane of 0.68 L, 45.6% methane more than the consortium treatment. A shift from Pseudomonas to Bacteroides was observed in the bacterial community at the genus taxonomic level after AD while Methanosarcina dominated the archaeal genera. Furthermore, independent bioaugmentation of AD of different substrates such as water hyacinth, cow dung, and cellulose powder with identified model bioaugmentation agent, Serratia marcescens portrayed a positive effect with regards to methane production when compared with the control treatment. This study represents an economic and environmentally friendly approach of single isolate inoculation (bioaugmentation) in optimizing methane production from lignocellulosic substrates such as water hyacinth.
Anaerobic digestion (AD) technology is limited by low-temperature conditions preventing the adoption of simple, unheated digesters during cold periods. Decrease in temperature has a detrimental effect on various AD operating parameters and imposes a strong negative impact on microbial growth and enzymatic activity, particularly among mesophiles and thermophiles. Psychrophiles, however, have evolved a range of genotypic and phenotypic adaptive features, which have enabled them to overcome barriers associated with cold environments. Consequently, much attention has focused on psychrophilic microorganisms and their metabolites such as enzymes, as biological augmentation tools for psychrophilic anaerobic digestion (PAD) due to their important economic and environmental benefits. However, such bioaugmentation capabilities, inherent in psychrophiles, are not fully exploited as only a few studies have been targeted towards their impact on PAD. Therefore, this review provides a holistic synopsis of the current state of knowledge on biological strategies to enhance AD performance, which may serve as a stepping-stone for future PAD-related research. The AD process under psychrophilic conditions and a global perspective of PAD is discussed. In addition, various adaptation strategies employed by psychrophiles to survive cold stress are identified. Finally, biological approaches including bioaugmentation as well as enzymatic biomethanation employed to address the problem of PAD are highlighted. Special focus is given to potential strategies that can be employed to improve the applicability of biological additives at low temperatures.
