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(a) Ascospores of Botryosphaeria dothidea. (b) Conidia of Botryosphaeria dothidea. Scale bar = 10 mm.
Source publication
Species of Botryosphaeria have been implicated in grapevine decline and dieback symptoms in Australian vineyards. In this study, we report the morphological and molecular identification of Botryosphaeria dothidea isolated from symptomatic grapevines in eastern grape growing regions of New South Wales, Australia. The pathogenicity of B. dothidea tow...
Similar publications
G. A., Diaz, J., Auger, X., Besoain, E., Bordeu, and B. A. Latorre, 2013. Prevalence and pathogenicity of fungi associated with grapevine trunk diseases in Chilean vineyards. Cien. Inv. Agr. 40(2): 327-339. Trunk diseases in grapevine (Vitis vinifera) have been identified as a major problem in the wine and table grape industries around the world, r...
A., Díaz, J., Auger, X., Besoain, E., Bordeu, and B. A. Latorre, 2013. Prevalence and
pathogenicity of fungi associated with grapevine trunk diseases in Chilean vineyards.
Cien. Inv. Agr. 40(2): 327-339. Trunk diseases in grapevine (Vitis vinifera) have been identified
as a major problem in the wine and table grape industries around the world, redu...
Citations
... Research findings suggest that within the Botryosphaeria genus of the Botryosphaeriaceae family, both N. parvum and B. dothodea are significant fungal species. N. parvum has been noted for causing cankers and diebacks in various woody species globally, while B. dothodea, although historically perceived as a mildly pathogenic fungus, is notably prevalent and cryptic in forestry, agriculture, and natural forest ecosystems (Phillips et al., 2005;Qiu et al., 2008;Marsberg et al., 2017). B. dothodea has been reported in various plant diseases such as apple ring rot , grapevine stem wilt (Li et al., 2010), leaf spot and lesions in horticultural plants (Cunnington et al., 2007), as well as bud blight and ulcer disease in pistachios (Wunderlich et al., 2012). ...
Background
Leaf spot disease severely impacts Ginkgo biloba (G. biloba) yield and quality. While microbial agents offer effective and non-toxic biological control for plant diseases, research on controlling leaf spot disease in G. biloba is notably scarce.
Methods
The pathogenic fungi were isolated and purified from diseased and healthy leaves of G. biloba, Subsequent examinations included morphological observations and molecular identification via PCR techniques. A phylogenetic tree was constructed to facilitate the analysis of these pathogenic fungi, and Koch’s postulates were subsequently employed to reaffirm their pathogenic nature. The antagonistic experiment was employed to select biocontrol bacteria, and subsequently, the isolated biocontrol bacteria and pathogenic fungi were inoculated onto healthy leaves to assess the inhibitory effects of the biocontrol bacteria.
Results
Two pathologies responsible for the leaf spot disease on G. biloba were identified as Botryosphaeria dothidea and Neofusicoccum parvum via the analysis of phylogenetic tree and the application of Koch’s Postulates. Additionally, we isolated two strains of biocontrol bacteria, namely Bacillus velezensis and Bacillus amyloliquefaciens. Their average inhibitory zones were measured at 4.78 cm and 3.46 cm, respectively. The inhibition zone of B. velezensis against N. parvum was 4 cm. B. velezensis showed a stronger inhibitory effect compared to B. amyloliquefaciens on the development of lesions caused by B. dothidea via leaf culture experiment.
Conclusion
This research reports, for the first time, the presence of B. dothidea as a pathogenic fungus affecting G. biloba. Moreover, the biocontrol bacteria, B. velezensis and B. amyloliquefaciens, exhibited the capability to effectively inhibit the growth and reproduction of B. dothidea, indicating their promising potential as environmentally friendly biocontrol resources.
... Sclerotium rolfsii is the pathogen responsible for southern blight, which is associated with the death of grapevines in nurseries (Keyser and Ferreira 1988). Botryosphaeria dothidea is linked to grapevine trunk disease (van Niekerk et al. 2006;Qiu et al. 2008;Li et al. 2010). Botrytis cinerea is the causal agent of gray mold disease on grapevine, which commonly occurs in cutting nurseries and grape production fields. ...
Grapevine (Vitis vinifera L.) is an economically important fruit crop in the world, and China ranks first in the production of grapes with approximately 15% of the world's total yield. However, diseases that cause the death of grapevine shoots pose a severe threat to the production of grapes. In this study, the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic analysis. The phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (Tef) and the β-tubulin (Tub2) genes. The three isolates were all identified as N. eucalypti. Pathogenicity tests of the three fungal isolates were conducted on grapevines shoots in vitro and in vivo. The results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots, and N. eucalypti was reisolated from the inoculated grapevine shoots. Therefore, N. eucalypti was confirmed as a causal agent of the grapevine shoot rot. This is the first report of N. eucalypti causing grapevine shoot disease in China.
... Because Bot infection frequency was negatively correlated with southwestness, this suggests that Bot growth might be facilitated on cooler, more northwesterly sites than hotter, more exposed sites in this region. This was a somewhat surprising result; Bots are widely known to grow well under high temperature (Qiu et al. 2008(Qiu et al. , 2016Úrbez-Torres 2006) and low water potential (Ma et al. 2001) environments like those that would be expected on exposed, southwest-facing sites. However, it is possible that the extreme conditions of these slopes during drought years might have been unfavorable for Bot growth, whereas these latent pathogens as endophytes could have benefitted from the slightly milder conditions of less exposed slopes. ...
Dieback and mortality in wildland plant species due to climate change has been on the rise in recent decades, and latent fungal pathogens may play a significant role in these events. During a severe multi-year drought, canopy dieback associated with latent pathogens in the Botryosphaeriaceae (Bot) family was observed in stands of a dominant shrub species, big berry manzanita (Arctostaphylos glauca), across chaparral landscapes in California. These fungi are significant pathogens of woody agricultural species, especially in hosts experiencing stress, and have become a threat to economically important crops worldwide. However, little is known regarding their occurrence, distribution and impact in wildland systems. We conducted a field survey of 300 A. glauca shrubs across an elevational gradient to identify Bot species infection as it relates to a) A. glauca dieback severity and b) landscape variables associated with plant drought stress. Our results show Bots widely infecting A. glauca across the landscape, and there is a significant correlation between elevation and dieback severity. Dieback severity was significantly higher at lower elevations, suggesting that infected shrubs at lower elevations are at greater risk than those at higher elevations. Furthermore, two Bot species, Neofusicoccum australe and Botryosphaeria dothidea, were most frequently isolated with N. australe being the most common and, based on haplotype analysis, likely the most recently introduced of the two. Our results confirm the wide distribution of latent Bot fungi in a wild shrubland system, and provide valuable insight into areas of greatest risk for future shrub dieback and mortality.
... B. dothidea is an endophytic fungus that infects healthy tissue of woody plants and remains dormant until the onset of abiotic stress, physical damage, waterlogging, frost and climate change conditions (Sakalidis et al. 2011;Marsberg et al. 2017). It has also been listed as one of the most widespread and important latent pathogens in a large number of plants in agriculture, forestry and forest systems (Pavlic et al. 2007;Qiu et al. 2008;Tang et al. 2012;Tian et al. 2018), among which are Alamo, Pine, Eucalyptus and Pistachio; for all the above, it is considered an opportunistic fungus (Ma et al. 2001;Taylor et al. 2009;P erez et al. 2010). Therefore, B. dothidea does not show any specificity to the host according to the ITS sequences, the elongation factor 1-a, and b-tubulin deposited in the GenBank database. ...
The diseases found in Pinus patula, a species present in the forest area of Puebla, Mexico cause foliage loss and damage to trees and we consider that fungi could be involved. The objective of the current investigation was to study the nutrients of the soil and the fungi associated with the lesions on the needles of Pinus patula in Tetela de Ocampo, Puebla, Mexico. It was found that the nitrogen, phosphorus and potassium content of the soils associated with pine, as well as the organic matter, were poor. In addition, we performed chemical, physical and biological analyses on soils from the study site. We isolated forty-five fungi from the lesions on the needles. The identification of twelve of them was both morphological and molecular by sequence analysis of the ITS1-ITS2 gene. The identified fungi included Alternaria alternata, Annulohypoxylon stygium, Botryosphaeria dothidea, Curvularia lunata, Curvularia pseudobrachyspora, Curvularia spicifera, Curvularia trifolii, Daldinia eschscholtzii, Daldinia sp. Diplodia sapinea, Lophodermium indianum and Myrmaecium rubricosum. We concluded that P. patula is affected by nutritional deficiency as well as by the presence of phytopathogenic fungi.
... Subsequent to the designation of the epitype, a number of species and even families have been separated from B. dothidea , Crous et al., 2006, Damm et al., 2007, Phillips et al., 2008. Despite the narrower species delimitation after epitypification, reports of B. dothidea during the last decade have confirmed that it is one of the most widespread and important endophytes or latent pathogens occurring on a large number of plants important in agriculture, forestry and in natural forest ecosystems (Jami et al., 2013a, Piškur et al., 2011, Slippers & 8 Wingfield, 2007, Pavlic et al., 2007, Phillips et al., 2005, Qiu et al., 2008, Tang et al., 2012, Xu et al., 2015. ...
... While the association of B. dothidea with such symptoms before 2004 must remain uncertain, recent studies have linked B. dothidea to similar symptoms on a variety of hosts. These include apple ring rot (Kim et al., 2004, Tang et al., 2012, Xu et al., 2015, fruit rot of olives (Phillips et al., 2005), grapevine trunk disease (Qiu et al., 2008, van Niekerk et al., 2006, Li et al., 2010, leaf spots and lesions on horticultural plants (Cunnington et al., 2007) as well as dieback and stem cankers on acacia (Gezahgne et al., 2004), eucalypt (Burgess et al., 2005, Gezahgne et al., 2004, Mohali et al., 2007, European hop hornbeam (Jurc et al., 2006), pine (Gezahgne et al., 2004), mango (Slippers et al., 2005), poplar (Grasso & Granata, 2010, Slippers et al., 2004b and stone fruit (Wang et al., 2011, Inderbitzin et al., 2010, Slippers et al., 2004b. Many more host associations and specific symptoms have been reported, but not confirmed using sequence data (Farr & Rossman, 2015). ...
Taxonomy:
Botryosphaeria dothidea (Moug. ex Fr) Ces. & De Not, 1863. Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Botryosphaeriaceae, Genus Botryosphaeria, Species dothidea Host range: Confirmed on more than 24 host genera, including woody plants such as Acacia (=Vachellia), Eucalyptus, Vitis and Pistachio.
Disease symptoms:
Associated with twig, branch and stem cankers, tip and branch dieback, fruit rot, blue stain and plant death This article is protected by copyright. All rights reserved.
... As an example, the virulence of Botryosphaeriaceae species on Oak (Quercus spp.) in Spain was found to be influenced by temperature (Sánchez et al., 2003). Furthermore, a preliminary study by Qiu et al. (2008) also indicated that B. dothidea was most virulent on detached canes at higher temperatures. ...
... For isolate DAR77825, the changes were more gradual and steady across the tested temperatures. As a result we can confirm the preliminary pathogenicity test by Qiu et al. (2008) which suggested that B. dothidea may be more virulent at higher temperatures. Furthermore, obvious intra-species variability in virulence was also observed in the potted grapevine trial with two isolates (DAR77825 and DAR78224) producing much longer lesions on the water stressed grapevines whilst DAR78225 produced a shorter lesion on the non-water-stressed grapevines. ...
Pathogenicity and virulence of the Botryosphaeriaceous fungi Botryosphaeria dothidea, Diplodia seriata, Lasiodiplodia theobromae and Neofusicoccum parvum were confirmed by inoculation of trunks and cordons on mature grapevines (Vitis vinifera 'Chardonnay'), inoculation of two-year-old potted grapevines in a glasshouse comparing water stressed versus non-water stressed vines, and by inoculation of one-year-old detached canes incubated at 25, 30 and 35°C. All four species were pathogenic with N. parvum the most virulent species followed by L. theobromae, B. dothidea and D. seriata in all pathogenicity assays. Incubation at 30°C resulted in increased virulence in most isolates. However, variation in virulence was observed for different isolates of the same species at 35°C. Significant intra- and inter-species variation in virulence was also observed between water stressed and non-water stressed vines. Optimal temperatures for colony growth and growth rate were also studied in an attempt to understand the variability observed in the pathogenicity tests. L. theobromae was the most rapidly growing species at the optimal growth temperature of 31.3°C followed by B. dothidea at 31.6, N. parvum at 30.2 and D. seriata at 27.6°C. At 25°C L. theobromae was also the fastest growing species with a growth rate of 1.07 mm h-1 followed by D. seriata at 0.87 mm h-1, N. parvum at 0.86 mm h-1 and B. dothidea at 0.83 mm h-1. Using these data the climatic prediction software, CLIMEX, was used to study the possible distribution of the four species in Australia.
... Ce dernier a été trouvé pour la première fois en 2008 en Europe du Sud. Ce champignon est connu pour provoquer des dépérissements sur un très grand nombre de plantes (Punithalingam 1976). Sa présence sur le continent européen pourrait faire apparaître dans les années à venir une nouvelle maladie qui pourrait toucher le vignoble français. ...
The article on fungal diseases of grapevine wood vine has been updated. This new version has been completed with the addition of a new chapter concerning the history of wood grapevine diseases from Ancient Times to the French Revolution (1789). A history is also described in each of the other chapters constituting this article. It includes more than 800 references and over 200 pictures.
... (Fig. 2). Morphological characteristics of the isolates were in agreement with the description for Fusicoccum aesculi Corda (Qiu et al. 2008). The identity of species was further confirmed by amplification and sequencing of the rDNA internal transcribed spacer region (ITS1-5.8S-ITS2) ...
... The sequence for the isolate CCTU 735 is available in GenBank with the accession number JQ663991. On the basis of molecular confirmation and morphological characteristics the fungus was identified as Botryosphaeria dothidea (Qiu et al. 2008). Excised shoot assay was used to evaluate pathogenicity of the isolates in laboratory condition. ...
Since 2008, there have been several reports on the occurrence of decline symptoms on grapevines in vineyards of East and West Azerbaijan provinces in Northern Iran by local farmers as well as plant protection clinics. The disease symptoms appeared as stunted growth, shoot dieback and wedge-shaped wood necrosis in cross section through the wood. Isolations were made from the surface sterilized tissues using routine plant pathology techniques. Based on the sequence data of ITS-rDNA and morphological characteristics of the conidia, the isolates were identified as Botryosphaeria dothidea. Pathogenicity assay using an excised shoot method revealed the isolates being pathogenic on grapevine. This is the first report on the occurrence of Botryosphaeria dothidea on grapevine in Iran.
... Botryosphaeria dothidea is a well known pathogen in a wide range of perennial woody hosts (Maas and Uecker 1984;Michailides 1991;Rumbos 1987), and reports of it on grapevines was given more attention recently. Millholland considered it to be an important pathogen that causes the rotting of ripening berries on muscadine grapevines (Miliholland 1991), Paradela Filho et al. reported B. dothidea causing a trunk canker of grapevines in Brazil (Filho et al. 1995), Phillips reported that B. dothidea and other fungi associated withdieback of grapevine in Portugal (Phillips 1998), and Qiu et al. also reported morphological and molecular identification of B. dothidea associated with grapevine trunk disease in south-eastern Australia (Qiu et al. 2008). In this study, both table and wine grapevine vineyards were surveyed, and samples with symptoms known to be associated with the disease, including dark brown discoloration on the trunk, vine canker, drying of fruit clusters, fruit drop, and diseased samples (vine, clusters and berry) were collected from many provinces in China. ...
Grapevine canker caused by Botryosphaeriaceae is currently one of most important trunk diseases in the world. This study identified one of the pathogens-B. dothidea that causes grapevine canker in most grapevine growing areas of China and verified its pathogenicity and geographical distribution. In total, 320 grapevine canker sympotoms samples were collected from 17 provinces between 2009 and 2011, and 4261 isolates were isolated from diseased samples. Among these, 1172 isolates purified from 150 samples were identified as B. dothidea by morphological and molecular characterizations. Three representative isolates were used to determine both optimal temperature and optimal nutrition medium for growth and germination. Pathogenicity of B. dothidea was determined in 25 grapevine cultivars. Pathogenicity results showed that most of the main grapevine cultivars growing in China are susceptible to the pathogen.
... Ces dépérissements sont observés en Afrique du Sud (van Niekerk et al. 2006), en Australie ( Castillo-Pando et al. 2001, Taylor et al. 2005, Savocchia et al. 2007b, Qiu et al. 2008), au Brésil ( Tavares et al. 1994, Carvalho Dias et al. 1998), au Canada (O" Gorman et al. 2009), en Californie ( Hewitt 1974, Úrbez-Torres et al. 2006a), au Chili (Morales et al. 2010), en Egypte (El-Goorani et El Meleigi 1972), en Espagne ( Martin et al. 2011b), en Italie ( Burruano et al. 2008), au Mexique ( Úrbez-Torres et al. 2008), en NouvelleZélande ( Amponsah et al. 2009, Baskarathevan et al. 2012a) et au Venezuela ( Ramirez et Pineda 2003) (fig. 30). ...
Les maladies du bois sont considérées comme très dommageables pour la pérennité du patrimoine viticole car les champignons responsables de ces maladies attaquent les organes pérennes de la vigne, provoquant à plus ou moins long terme la mort du cep. Dans le vignoble, elles se manifestent par différentes symptomatologies au niveau de la partie herbacée. Elles peuvent se caractériser par des formes plus ou moins sévères allant jusqu"à l"apoplexie ou par des formes lentes conduisant à l"affaiblissement progressif de la plante (perte de vigueur), pour aboutir à la mort d"une de ses parties (coursons, bras), puis à sa totalité. Ces dernières s"expriment soit par des zones nécrotiques sur les feuilles se traduisant par quelques taches ou par des zones beaucoup plus importantes donnant un aspect de tigrure à la feuille, soit par des rabougrissements de la végétation. Selon leur gravité ou la période pendant laquelle elles se manifestent, elles peuvent toucher les inflorescences ou les fruits. Ces derniers peuvent ne pas atteindre leur maturation, se dessécher ou encore prendre un aspect millerandé. Les fruits peuvent aussi se tacheter rendant ainsi non commercialisable les raisins de table. Il est également possible d"observer la mort de la plante sans qu"il y ait expression de symptômes visibles. Dans le bois, ces maladies se traduisent par différentes nécroses qui sont plus ou moins développées allant de quelques vaisseaux obstrués jusqu"à la formation de chancres. Le bois peut présenter différents aspects de dégradation. Ces maladies peuvent toucher les jeunes plantations et les vignes plus âgées. Cet article fait le point sur leur symptomatologie et les agents qui y sont associés, ou qui en sont responsables. Les maladies (Pourridiés…) se caractérisant par une attaque du collet ou des racines pouvant se traduire par des symptômes similaires ne seront pas abordées dans cet article.
