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Wiesneriomyces laurinus. A. Sporodochia on natural substratum. B-C. Sporodochia with incurved setae arising from a prominent sporodochial stalk. D. Branched conidiophores. Scale bars: A-C = 20 µm, D = 10 µm.
Source publication
Wiesneriomyces is an asexual genus comprising two species, found growing on submerged leaves and terrestrial leaf litter in tropical habitats. The genus is characterized by conidiomata with setae, macronematous and branched conidiophores, and hyaline conidia in uniseriate chains connected by narrow isthmi. A multigene (SSU & LSU) analysis of 13 str...
Context in source publication
Context 1
... laurinus (Tassi) P.M. Kirk 1984 (Figs 2, 3) = Volutellaria laurina Tassi 1897 MycoBank MB 107371 Saprobic on leaf litter. Sexual state: Unknown. Asexual state: Colonies effuse, olivaceous-brown to dark brown. Conidiomata sporodochial, dispersed, on a dark pseudoparenchymatous stalk, consisting of 2-3 µm, dark, inflated, thick-walled cells. Setae subulate, apex acute, septate, thick-walled, pale brown, arising at the margins of the stalk ...
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Citations
... The order Tubeufiales was introduced by Boonmee et al. (2014) to accommodate the type family, Tubeufiaceae, based on molecular phylogenetic studies. Subsequently, Suetrong et al. (2014) introduced the second family Wiesneriomycetaceae under Tubeufiales based on morphological and DNA sequence data. However, Bezerra et al. (2017) elevated Wiesneriomycetaceae to a new order, Wiesneriomycetales. ...
... Morphological characterization and phylogenetic reconstruction have shown that members of the Wiesneriomyces clade form a monophyletic clade in the class Dothideomycetes, a sister lineage to Tubeufiales , Tsui et al. 2006a, Boonmee et al. 2011, Zhang et al. 2011, Hyde et al. 2013. Hence, based on multigene phylogenetic analyses, Suetrong et al. (2014) formally introduced Wiesneriomycetaceae to accommodate Wiesneriomyces s. str. This was supported by culture and morphological data as well. ...
... This was supported by culture and morphological data as well. Nevertheless, Wiesneriomycetaceae (Suetrong et al. 2014) was invalid since they used an incorrect identifier (Art. 42.1). ...
The monotypic genus Excipulariopsis, with type species E. narsapurensis, was established based on the emended generic characteristics of Excipularia narsapurensis. Excipulariopsis has non-cupulated superficial conidiomata arising directly from stroma surrounded by thick walled dark brown subulate setae. The conidia are dark brown, broadly fusoid, having thick septa and truncate base. Phylogenetic analyses using ITS, LSU, TEF1α and RPB2 sequences positioned Excipulariopsis allied to Speiropsis in the family Wiesneriomycetaceae, as a sister family to Tubeufiaceae within Tubeufiales. This study resolved a long-standing ambiguity about the phylogenetic status of this unique sporodochial setose hyphomycetes from India.
... Based on morphology and molecular phylogeny, Suetrong et al. (2014) introduced Wiesneriomycetaceae as order incertae sedis. Pratibha et al. (2015) placed Wiesneriomycetaceae in Tubeufiales. ...
... Ex-type strains are in bold and newly generated sequences are in red. Bootstrap support values for ML ≥ 50% and BYPP ≥ 0.95 are given above the nodes laurinus (Heredia et al. 2000;Rajashekhar and Kaveriappa 2000;Suetrong et al. 2014;Pratibha et al. 2015;Tennakoon et al. 2021). The phylogeny also showed that our strains (ZHKUCC 22-008 and ZHKUCC 22-009) clustered with other Wiesneriomyces laurinus strains (closer to BCC18609), with moderate statistical support (Fig. 54). ...
This article is the 14th in the Fungal Diversity Notes series, wherein we report 98 taxa distributed in two phyla, seven classes, 26 orders and 50 families which are described and illustrated. Taxa in this study were collected from Australia, Brazil, Burkina Faso, Chile, China, Cyprus, Egypt, France, French Guiana, India, Indonesia, Italy, Laos, Mexico, Russia, Sri Lanka, Thailand, and Vietnam. There are 59 new taxa, 39 new hosts and new geographical distributions with one new combination. The 59 new species comprise Angustimassarina kunmingense, Asterina lopi, Asterina brigadeirensis, Bartalinia bidenticola, Bartalinia caryotae, Buellia pruinocalcarea, Coltricia insularis, Colletotrichum flexuosum, Colletotrichum thasutense, Coniochaeta caraganae, Coniothyrium yuccicola, Dematipyriforma aquatic, Dematipyriforma globispora, Dematipyriforma nilotica, Distoseptispora bambusicola, Fulvifomes jawadhuvensis, Fulvifomes malaiyanurensis, Fulvifomes thiruvannamalaiensis, Fusarium purpurea, Gerronema atrovirens, Gerronema flavum, Gerronema keralense, Gerronema kuruvense, Grammothele taiwanensis, Hongkongmyces changchunensis, Hypoxylon inaequale, Kirschsteiniothelia acutisporum, Kirschsteiniothelia crustaceum, Kirschsteiniothelia extensum, Kirschsteiniothelia septemseptatum, Kirschsteiniothelia spatiosum, Lecanora immersocalcarea, Lepiota subthailandica, Lindgomyces guizhouensis, Marthe asmius pallidoaurantiacus, Marasmius tangerinus, Neovaginatispora mangiferae, Pararamichloridium aquisubtropicum, Pestalotiopsis piraubensis, Phacidium chinaum, Phaeoisaria goiasensis, Phaeoseptum thailandicum, Pleurothecium aquisubtropicum, Pseudocercospora vernoniae, Pyrenophora verruculosa, Rhachomyces cruralis, Rhachomyces hyperommae, Rhachomyces magrinii, Rhachomyces platyprosophi, Rhizomarasmius cunninghamietorum, Skeletocutis cangshanensis, Skeletocutis subchrysella, Sporisorium anadelphiae-leptocomae, Tetraploa dashaoensis, Tomentella exiguelata, Tomentella fuscoaraneosa, Tricholomopsis lechatii, Vaginatispora flavispora and Wetmoreana blastidiocalcarea. The new combination is Torula sundara. The 39 new records on hosts and geographical distribution comprise Apiospora guiyangensis, Aplosporella artocarpi, Ascochyta medicaginicola, Astrocystis bambusicola, Athelia rolfsii, Bambusicola bambusae, Bipolaris luttrellii, Botryosphaeria dothidea, Chlorophyllum squamulosum, Colletotrichum aeschynomenes, Colletotrichum pandanicola, Coprinopsis cinerea, Corylicola italica, Curvularia alcornii, Curvularia senegalensis, Diaporthe foeniculina, Diaporthe longicolla, Diaporthe phaseolorum, Diatrypella quercina, Fusarium brachygibbosum, Helicoma aquaticum, Lepiota metulispora, Lepiota pongduadensis, Lepiota subvenenata, Melanconiella meridionalis, Monotosporella erecta, Nodulosphaeria digitalis, Palmiascoma gregariascomum, Periconia byssoides, Periconia cortaderiae, Pleopunctum ellipsoideum, Psilocybe keralensis, Scedosporium apiospermum, Scedosporium dehoogii, Scedosporium marina, Spegazzinia deightonii, Torula fici, Wiesneriomyces laurinus and Xylaria venosula. All these taxa are supported by morphological and multigene phylogenetic analyses. This article allows the researchers to publish fungal collections which are important for future studies. An updated, accurate and timely report of fungus-host and fungus-geography is important. We also provide an updated list of fungal taxa published in the previous fungal diversity notes. In this list, erroneous taxa and synonyms are marked and corrected accordingly.
... ITS region does not seem suitable to resolve the phylogeny of Wiesneriomyces laurinus, since isolates of this species were split into three groups with other species in between (Speiropsis spp., Phalangispora nawawii, and P. constricta). The analysis of both 18S and 28S regions seems to have better resolving power for Wiesneriomyces laurinus [62]. We also found problems with a few isolates that did not group together with the remaining isolates of the same species, possibly due to misidentification: Amniculicola longissima WPRHD03, Neonectria lugdunensis NRRL-20592, Flagellospora curvula 30-67, Anguillospora furtiva NBRC-103659, Varicosporium elodeae AU-CRYP05, and Articulospora tetracladia CCM F-12313. ...
Aquatic hyphomycetes are key microbial decomposers of allochthonous organic matter in
freshwater ecosystems. Although their importance in carbon flow and food webs in streams is widely
recognized, there are still gaps in our understanding of their molecular diversity and distribution
patterns. Our study utilized the growing database of ITS rDNA barcodes of aquatic hyphomycetes
(1252 sequences) and aimed to (i) produce new barcodes for some lesser-known taxa; (ii) clarify
the taxonomic placement of some taxa at the class or order level, based on molecular data; and
(iii) provide insights into the biogeographical origins of some taxa. This study increased the number
of aquatic hyphomycete species with available ITS barcodes from 119 (out of ~300 species described)
to 136. Phylogenetically, the 136 species were distributed between 2 phyla, 6 classes, and 10 orders of
fungi. Future studies should strive to increase the database of ITS sequences, especially focusing on
species with unclear phylogenetic relationships (incertae sedis) and with few sequences available. The
geographical distribution of species with available ITS sequences included 50 countries from five
continents, but 6 countries had more than 20 species associated, showing a bias toward the northern
hemisphere, likely due to sampling bias.
... ITS region does not seem suitable to resolve the phylogeny of Wiesneriomyces laurinus, since isolates of this species were split into three groups with other species in between (Speiropsis spp., Phalangispora nawawii and P. constricta). The analysis of both 18S and 28S regions seems to have better resolving power for Wiesneriomyces laurinus (Suetrong et al. 2014). We also found problems with a few isolates that did not group together with the remaining isolates of the same species, possibly due to misidenti cation: Amniculicola longissima WPRHD03, Neonectria lugdunensis NRRL-20592, Flagellospora curvula 30-67, Anguillospora furtiva NBRC-103659, Varicosporium elodeae AU-CRYP05 and Articulospora tetracladia CCM F-12313, ...
Aquatic hyphomycetes are key microbial decomposers of allochthonous organic matter in freshwater ecosystems. Although their importance in carbon ow and food webs in streams is widely recognized, there are still gaps in our understanding of their phylogeny, molecular diversity and distribution patterns. Our study utilized the growing database of ITS rDNA barcodes of aquatic hyphomycetes (1252 sequences) and aimed to (i) produce new barcodes for some lesser-known taxa of aquatic hyphomycetes, (ii) clarify the phylogeny of aquatic hyphomycetes, including the placement of some taxa at the class or order level based on molecular data, and (iii) provide insights into biogeography of this group. This study increased the number of aquatic hyphomycetes with available ITS barcode from 120 species (out of ~ 300 species described) to 136 species. Phylogenetically, the 136 species of aquatic hyphomycetes were distributed between two phyla (Ascomycota and Basidiomycota), 6 classes and 10 orders of fungi. However, 36 species remain incertae sedis without attribution at the order level. The geographical distribution of species with available ITS sequences included 50 countries from 5 continents. North American and European countries showed the highest number of aquatic hyphomycete species with ITS barcodes, with 6 countries having more than 20 species: Portugal (34 species), United States of America (33 species), United Kingdom (29 species), Germany (28 species), France (22 species) and Czech Republic (21 species).
... Decomposing fungi Saprotrophy Acremonium a [197] Alternaria a [198] Arthrinium [199] Aspergillus a [200] Avachytrium b [201] Capnobotryella b [202] Chaetospermum [203] Chytriomyces [204] Plants 2021, 10 [205] Emericellopsis [206] Entophlyctis b [207] Fusarium a [208] Galactomyces [209] Gibellulopsis a [210] Glutinoglossum b [211] Hannaella a [212] Helicascus [213] Inocybe b [214] Lentithecium [215] Mortierella a [216] Nowakowskiella [217] Ochroconis a [218] Piromyces [219] Pyrenochaeta a [220] Sporobolomyces a [221] Wiesneriomyces [222] Parasitism ...
Cladophora is an algal genus known to be ecologically important. It provides habitats for microorganisms known to provide ecological services such as biosynthesis of cobalamin (vitamin B12) and nutrient cycling. Most knowledge of microbiomes was obtained from studies of lacustrine Cladophora species. However, whether lotic freshwater Cladophora microbiomes are as complex as the lentic ones or provide similar ecological services is not known. To illuminate these issues, we used amplicons of 16S rDNA, 18S rDNA, and ITS to investigate the taxonomy and diversity of the microorganisms associated with replicate Cladophora samples from three sites along the Nan River, Thailand. Results showed that the diversity of prokaryotic and eukaryotic members of Cladophora microbiomes collected from different sampling sites was statistically different. Fifty percent of the identifiable taxa were shared across sampling sites: these included organisms belonging to different trophic levels, decomposers, and heterotrophic bacteria. These heterogeneous assemblages of bacteria, by functional inference, have the potential to perform various ecological functions, i.e., cellulose degradation, cobalamin biosynthesis, fermentative hydrogen production, ammonium oxidation, amino acid fermentation, dissimilatory reduction of nitrate to ammonium, nitrite reduction, nitrate reduction, sulfur reduction, polyphosphate accumulation, denitrifying phosphorus-accumulation, and degradation of aromatic compounds. Results suggested that river populations of Cladophora provide ecologically important habitat for microorganisms that are key to nutrient cycling in lotic ecosystems.
... Foraging M. bellicosus workers encase their foraging material in a thin sheet of soil and close it off from the environment (Fig. 1b), but the fungal diversity we observed below these sheets was appreciable, suggesting that this environment is not sanitised by the foraging workers. Unsurprisingly, many of the most abundant genera, such as Hypoxylon, Allocryptovalsa and Wiesneriomyces (Fig. 1c), are wood decomposers [37][38][39] and they may thus compete with Termitomyces if they enter fungal combs. Although in low abundance, members of the family Xylariaceae appeared in all M. bellicosus foraging materials. ...
Background
Monoculture farming poses significant disease challenges, but fungus-farming termites are able to successfully keep their monoculture crop free from contamination by other fungi. It has been hypothesised that obligate gut passage of all plant substrate used to manure the fungal symbiont is key to accomplish this. Here we refute this hypothesis in the fungus-farming termite species Macrotermes bellicosus .
Results
We first used ITS amplicon sequencing to show that plant substrate foraged on by termite workers harbour diverse fungal communities, which potentially could challenge the farming symbiosis. Subsequently, we cultivated fungi from dissected sections of termite guts to show that fungal diversity does not decrease during gut passage. Therefore, we investigated if healthy combs harboured these undesirable fungal genera, and whether the presence of workers affected fungal diversity within combs. Removal of workers led to a surge in fungal diversity in combs, implying that termite defences must be responsible for the near-complete absence of other fungi in functioning termite gardens.
Conclusions
The rapid proliferation of some of these fungi when colonies are compromised indicates that some antagonists successfully employ a sit-and-wait strategy that allows them to remain dormant until conditions are favourable. Although this strategy requires potentially many years of waiting, it prevents these fungi from engaging in an evolutionary arms race with the termite host, which employs a series of complementary behavioural and chemical defences that may prove insurmountable.
... Thus, a new order, Tubeufiales, was established to accommodate the Tubeufiaceae comprising 19 genera (Boonmee et al. 2014). Wiesneriomycetaceae was introduced in Tubeufiales by Suetrong et al. (2014), but Bezerra et al. (2017) rejected this family. However, Liu et al. (2017) justified Bezerromycetaceae (Bezerra et al. 2017) and ...
Kevinhydea brevistipitata gen. et sp. nov., and Helicoma hydei sp. nov., collected on decaying wood in terrestrial and freshwater habitats, respectively, are described and illustrated. Kevinhydea brevistipitata, the type species for a novel monotypic genus (Kevinhydea), is characterized by its macronematous, short conidiophores, and dictyosporous conidia. Helicoma hydei morphologically resembles H. guttulatum, but they are phylogenetically distinct species. Phylogenetic analyses of combined LSU, ITS, and TEF1α sequence data of 63 taxa were carried out to infer the phylogenetic affinity of new taxa within the family Tubeufiaceae. Additionally, phylogenetic analyses for the genus Helicoma, comprising 33 taxa, were performed separately to justify the new species H. hydei. Kevinhydea brevistipitata and Helicoma hydei are phylogenetically distinct from other species in Tubeufiaceae. A morphological comparison of dictyosporous hyphomycete genera in Tubeufiaceae is provided.
... The order Tubeufiales was introduced by Boonmee et al. (2014) to accommodate the type family, Tubeufiaceae, based on molecular phylogenetic studies. In the same year, Suetrong et al. (2014) introduced a second family Wiesneriomycetaceae to Tubeufiales based on morphological and DNA sequence data. Bezerra et al. (2017) excluded Wiesneriomycetaceae from Tubeufiales based on phylogenetic analysis. ...
This study deals with an extensive taxonomic reevaluation focusing on phylogenetic relationships and morphological characterization of Tubeufiales, especially those helicosporous hyphomycetes which are difficult to identify. Based on evidence from DNA sequence data and morphology, we introduce 13 new genera in the family Tubeufiaceae, viz. Acanthotubeufia, Dematiohelicoma, Dematiohelicomyces, Dematiohelicosporum, Dematiotubeufia, Helicoarctatus, Helicohyalinum, Helicotruncatum, Neochlamydotubeufia, Neohelicoma, Pleurohelicosporium, Pseudohelicomyces and Pseudohelicoon; transfer Chaetosphaerulina from Dothideomycetes genera incertae sedis, and Artocarpomyces and Helicodochium from Ascomycetes genera incertae sedis into Tubeufiaceae; introduce 52 new species, viz. Berkleasmium fusiforme, B. longisporum, Chlamydotubeufia cylindrica, Dematiohelicosporum guttulatum, Helicoarctatus aquaticus, Helicodochium aquaticum, Helicohyalinum infundibulum, Helicoma aquaticum, H. brunneisporum, H. cocois, H. rufum, H. fusiforme, H. longisporum, H. multiseptatum, H. rubriappendiculatum, H. septoconstrictum, H. tectonae, Helicomyces hyalosporus, Helicosporium aquaticum, H. flavisporum, H. setiferum, H. vesicarium, H. viridiflavum, Neochlamydotubeufia fusiformis, Neohelicomyces hyalosporus, Neohelicosporium acrogenisporum, N. astrictum, N. ellipsoideum, N. irregulare, N. krabiense, N. laxisporum, N. ovoideum, Pleurohelicosporium parvisporum, Pseudohelicomyces aquaticus, P. hyalosporus, Tubeufia abundata, T. bambusicola, T. brevis, T. brunnea, T. chlamydospora, T. dictyospora, T. eccentrica, T. fangchengensis, T. hechiensis, T. inaequalis, T. krabiensis, T. rubra, T. sessilis, T. sympodihylospora, T. sympodilaxispora, T. taiwanensis and T. tratensis; provide 43 new combinations, viz. Acanthohelicospora guianensis, Acanthotubeufia filiforme, Berkleasmium aquatica, B. guangxiense, B. latisporum, B. thailandicum, Dematiohelicoma perelegans, D. pulchrum, Dematiohelicomyces helicosporus, Dematiotubeufia chiangraiensis, Helicohyalinum aquaticum, Helicoma elinorae, H. gigasporum, H. hongkongense, H. linderi, H. nematosporum, H. pannosum, H. serpentinum, Helicomyces chiayiensis, Helicotruncatum palmigenum, Neochlamydotubeufia khunkornensis, Neohelicoma fagacearum, Neohelicomyces pallidus, Neohelicosporium abuense, N. aurantiellum, N. griseum, N. morganii, N. myrtacearum, N. nizamabadense, N. sympodiophorum, N. taiwanense, N. vesiculiferum, Pseudohelicomyces indicus, P. paludosus, P. talbotii, Pseudohelicoon gigantisporum, P. subglobosum, Tubeufia dentophora, T. geniculata, T. lilliputea, T. machaerinae, T. sympodiophora and T. xylophila; introduce 16 new records, viz. Dictyospora thailandica, Helicomyces colligatus, H. torquatus, Neohelicosporium guangxiense, N. hyalosporum, N. parvisporum, Thaxteriellopsis lignicola, Tubeufia aquatica, T. chiangmaiensis, T. cylindrothecia, T. filiformis, T. guangxiensis, T. laxispora, T. parvispora, T. roseohelicospora and T. tectonae. The taxonomy of Helicoma, Helicomyces and Helicosporium is revisited based on phylogenetic analyses and morphological evidence. Neorhamphoria is transferred to Bezerromycetaceae. Three species are excluded from the genus Chlamydotubeufia, twelve species from Helicoma, four species from Helicomyces, 25 species from Helicosporium, six species from Neoacanthostigma and one species from Tubeufia. A multi-gene phylogenetic tree based on maximum likelihood and Bayesian analyses of ITS, LSU, RPB2 and TEF1α sequence data of species of Tubeufiales is provided. Detailed descriptions and illustrations are provided, as well as the morphological comparison with similar taxa are explored. The checklist of accepted Tubeufiales species and re-organised Tubeufiales species are provided.
... Morphologically, W. machilicola can be distinguished from the two other species by its conidial size, setae, and conidiophores: W. laurinus has longer conidia (50-90 µm, with 6-8 cells) and much longer setae (≤476 µm; Maniotis & Strain 1968, Matsushima 1971, while W. conjunctosporus has much longer (280-360 µm) conidia with up to 15 cells and much longer (≤600 µm) setae (Kuthubutheen & Nawawi 1988). Suetrong et al. (2014) ...
A new microfungus collected from dead leaves of Machilus ichangensis in China is described and illustrated as Wiesneriomyces machilicola (Wiesneriomycetaceae, Dothideomycetes). The species is distinguished by 3-6-celled, straight or falcate, hyaline conidia, 35-45 μm long. Mycotaxon, Ltd.
... In addition, representative sequences from the family Wiesneriomycetaceae, proposed by Suetrong et al. (2014) as order incertae sedis, represented a strongly supported clade closely related to the new order Bezerromycetales and Tubeufiales. In their study, the phylogenetic analyses of Suetrong et al. (2014) showed Wiesneriomycetaceae as a monophyletic clade between Patellariales and Tubeufiales, with Tubeufiales as closest sister group. ...
... In addition, representative sequences from the family Wiesneriomycetaceae, proposed by Suetrong et al. (2014) as order incertae sedis, represented a strongly supported clade closely related to the new order Bezerromycetales and Tubeufiales. In their study, the phylogenetic analyses of Suetrong et al. (2014) showed Wiesneriomycetaceae as a monophyletic clade between Patellariales and Tubeufiales, with Tubeufiales as closest sister group. This family is morphologically characterized by sporodochial conidiomata with setae, macronematous and branched conidiophores, and hyaline to slightly pigmented conidia in uniseriate chains connected by narrow isthmi, lacking a sexual morph . ...
During a survey of endophytic fungi from the cactus Tacinga inamoena in a Brazilian tropical dry forest (Caatinga) some undescribed ascomycetous fungi were isolated. These fungi are characterized by superficial and immersed, globose to subglobose, smooth or hairy ascomata, bitunicate asci, and muriformly septate, ellipsoidal ascospores. Multigene phylogenetic analyses using sequences from partial ITS, SSU and LSU nrDNA and the translation elongation factor 1-alpha gene (tef1) demonstrated a monophyletic clade accommodating these endophytic fungi in the class Dothideomycetes, closely related to the order Tubeufiales. Based on morphological features and phylogenetic analyses, these fungi could not be placed in the order Tubeufiales, in the new order Wiesneriomycetales, or any other known genus in the class Dothideomycetes. Thus, two new genera (Bezerromyces, with B. brasiliensis and B. pernambucoensis, and Xiliomyces with X. brasiliensis), a new family (Bezerromycetaceae) and a new order (Bezerromycetales) are introduced to accommodate these novel taxa. Our phylogenetic analyses also demonstrated that the clade accommodating Wiesneriomycetaceae represents a new order, here introduced as Wiesneriomycetales.
