Figure 3 - uploaded by Makeshkumar T
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Viral RNA genome of DsMV showing conserved regions for Potyviral amplification using MJ1 and MJ2 and MJ1 and M4T.
Contexts in source publication
Context 1
... RT-PCR of the total RNA isolated from infected Elephant foot yam leaves with MJ1 and MJ2 primers, which amplifies the core region of the coat protein of Potyviral genomes (Figure 3), resulted in the amplification of 327 bp partial coat protein gene (Figure 4). The amplified product was cloned, sequenced and the ...
Context 2
... RNA isolated from infected Elephant foot yam leaves when subjected to one-step RT-PCR with M4T and MJ1 primers, a 719 bp product was obtained ( Figure 5). The primers MJ1 and MJ2 amplified an internal partial region of the coat protein, while the primer M4T in combination with MJ1 amplified the further C-terminal region of the coat protein and 3 0 UTR region of the virus (Figure 3). The product was cloned, sequenced and the sequence deposited in GenBank (accession number EU375290). ...
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